绿僵菌

该基因在绿僵菌基因组以单拷贝形式存在，DNA序列含有两个内含子。

The gene existed one copy in the fungi ` s genome , and its DNA sequence contained two introns .

绿僵菌孢子活性的MTT比色法快速检测技术研究

Rapid MTT colorimetric assay for the detection of Metarhizium anisopliae spore viability

测定了不同温度和pH及碳氮源、微量元素对金龟子绿僵菌菌丝生长及产孢的影响。

A strain of Metarhizium anisopliae was incubated in solid culture under different conditions of temperature , pH , carbon and nitrogen sources .

用gfp基因标记法研究金龟子绿僵菌在玉米根际的定殖动态

Colonization of Metarhizium anisopliae in Corn Rhizosphere as Studied with gfp Marker Gene

通过正交设计试验优化了绿僵菌RAPD-PCR反应体系，并对不同地理来源金龟子绿僵菌进行DNA多态性RAPD分析。

Optimized RAPD-PCR reaction system by orthogonal design , and DNA polymorphism for RAPD analysis of Metarhizium anisopliae isolate from different areas .

绿僵菌Metarhizium是防治害虫很有潜力的病原物，在土壤中蕴藏丰富。

Metarhizium is a great potential pest control pathogenic microorganisms and rich in the soil .

在平板上测定了不同温度、pH值、碳氮源、微量元素及生长因子（维生素）对绿僵菌菌丝生长及产孢量的影响。

The effect of temperature , pH , source of carbon and nitrogen , trace element and growth factor ( vitamine ) on the growth of mycelium and sporulation of Metarhizium was tested on the culture medium .

金龟子绿僵菌（Metarhiziumanisopliae）是在害虫生物防治中起着重要作用的一类重要的昆虫病原真菌。

Metarhizium anisopliae is playing an important role in biological control of pests of an important class of insect pathogenic fungi .

目的：对金龟子绿僵菌的代谢产物进行初步的研究，验证其产物中苦马豆素（swainsonine，SW）的存在，为SW的来源提供新的研究思路；

Subject : Provide a new thoughtfulness for preliminary studying the source of swainsonine ( SW ) and validate SW in the metabolism products of Metarhizium anisopliae .

目的研究生物农药绿僵菌（Metarhizium）原药急性经口、经皮、吸入毒性及眼睛刺激和致敏率。

Objective To test the acute toxicity of biopesticide-Metarhizium , include oral toxicity , dermal toxicity , inhalation toxicity , eye irritation and dermal sensitization .

微波处理固体培养基120s或过氧化氢质量分数1.0%，微波处理90s，接种培养绿僵菌能够正常生长和产孢。

Metarhizium anisopliae could grow well on the solid medium when treated with microwave 120 seconds or 90 seconds in company with 1.0 % hydrogen peroxide .

利用绿僵菌Metarrhiziumanisopliae的发酵过程研究了菌丝体形态与菌体生长的关系。

The fermentation with mold metarrhizium anisopliae has been carried out to determine the relationship between mycelial morphology and growth .

金龟子绿僵菌（Metarhiziumanisopliae）能侵染多种鳞翅目昆虫，广泛用于生物防治，是最常用的生物农药之一。

For its large host-range which includes many kinds of lepidoptera and its large scale use for bio-control , Metarhizium anisopliae have been one of bio-pesticides for using widely .

药后45d，绿僵菌对铜绿丽金龟幼虫蛴螬的防效和对地下害虫的总防效分别为91.2%和87.6%。

Forty-five d after application , M. anisopliae provided 91.2 and 87.6 % control of immature grubs ( Anomalacorpulenta ) and total subterranean insect pests , respectively .

本文用7个绿僵菌（Metarhizium）菌株对德国小蠊Blattellagermanica（Linnaeus）进行注射生测，筛选出了一个致病菌株进行五代复壮选育。

The virulence of 7 Metarhizium strains against the German cockroaches Blattella germanica ( Linnaeus ) was selected by injection method , and a pathogenic strain Metarhizium anisopliae ( Metsch ) Sorokin was passed through five times rejuvenation .

产烟曲霉酸等甾型抗生素的真菌，例如烟曲霉和金龟子绿僵菌等，具有优良的甾体羟化和C1,2位脱氢能力。

Aspergillus fumigatus and Metarhizium anisopliae , fungi which produce steroid antibiotics like helvolic acid , have a great capability for steroid hydroxylation and C1,2 dehydrogenation .

结论微波对绿僵菌固体培养基灭菌最佳工艺为：100g的固体培养基加60g水，浸润3h，在800W的微波功率处理3min，可达到灭菌效果。

Conclusion The best technology of microwave sterilization of Metarhizium anisopliae solid culture medium was adding water 60 g to solid culture medium 100 g , infiltration for 3 h and 800 W microwave treatment for 3 min. Sterilization can be achieved by this technology .

经过优化，得到金龟子绿僵菌产孢的最佳生物参数是：接种量为2.5g/100g、种龄为5-6天；

The technology of two - stage fermentation was also studied . The results showed that optimal biological conditions of solid - state fermentation of Metarhizium anisopliae were as following : inoculation ratio 2.5g/100g and seed age 6 days .

绿僵菌对高寒地区草原蝗虫的防治效果试验

Metarhizium Anisopliae on The Alpine Area of Grassland Locust Control Tes

不同的温湿度下绿僵菌对马铃薯瓢虫的防治效果也不相同。

The control effect was different under different temperature and humidity .

绿僵菌高致病性菌株的筛选

Screening of pathogenic strains of Metarhizium anisopliae from the field isolates

金龟子绿僵菌孢子制备、贮存及检测标准化研究

Standardization of Preparation , Storage and Inspection of Metarhizium Anisopliae Conidia

金龟子绿僵菌对甾体底物11-α羟化反应的工艺

11 - α hydroxylation Process of Steroids with Metarhizium anisopliae

通过对菌株形态学鉴定，获得金龟子绿僵菌29株。

The strains were identified of Metarhizium anisopliae by morphology . 2 .

绿僵菌的急性毒性及致敏实验观察

The Acute Toxicity and Dermal Sensitisation of Metarhizium Original Drug

应用绿僵菌复合剂防治桉树白蚁的几个技术问题

Study on the Controlling Techniques of Eucalyptus Termites with Metarhizium anisopliae Complex

绿僵菌在土壤中宿存形式和存活时间的测定

Persistent Forms and Survival Potential of Metarhizium anisopliae in Soil

这两处是利于绿僵菌侵入的薄弱环节。

The two places were vulnerable to M. anisopliae invasion .

绿僵菌主要通过表皮直接侵染虫体。

The fungi infected insect body mainly through cuticle .

绿僵菌的培养及其防治白蚁的效果

The Culture of Metarhizium and Its Effect Against Termites