胭脂碱
- 名nopaline
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把带有甜蛋白基因和胭脂碱合成酶标记基因的Ti质粒导入马铃薯,获得大量转化植株。
A number of transgenic plants were obtained using the plasmid containing a sweet protein NPT ⅱ and nopaline synthase genes .
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应用根癌农杆菌胭脂碱合成酶基因(nos)终止子和大肠杆菌K12菌株新霉素磷酸转移酶Ⅱ(nptⅡ)基因的二重PCR对样品进行转基因成分检测,结果均为阴性。
The duplex PCR detection results of Agrobacterium tumefaciens nopaline synthase ( nos ) terminator and Escherichia coli strain K12 neomycin phosphotransferase II ( npt ⅱ) gene in these samples were all negative .
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A(84)的敏感性与胭脂碱Ti质粒及宿主的关系
A_ ( 84 ) sensitivity determined by both nopaline type Ti plasmid and its host cell
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GUS活性检测和胭脂碱分析表明,外源基因在转化细胞内得到稳定表达。
Enzyme activity of GUS and NOS assay demonstrated that foreign genes expressed in the transformed plants and calli .
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花生畸胎瘤冠瘿瘤的诱导及胭脂碱合成酶基因的转移
Teratoma Induction and the Nopaline Synthase Gene Transfer in Peanut
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大豆属致瘤农杆菌致瘤后胭脂碱的测定
Determination on nopaline dehydrogenase from tumor callus of soybean plants caused by
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取转化苗叶片作胭脂碱合成酶活性检测电泳后呈阳性;
The nopaline synthetic enzyme activity of the preliminary transformation was detected by electrophoresis .
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其中以开花初期的植株,利用胭脂碱型农杆菌介导(如C58),真空渗入5分钟的处理方法转化效率最高。
It was concluded that using the nopaline type of Agrobacterium ( e.g.C58 ), 5min vacuum infiltration , and using the plants with just several blooming flowers would give the best results .