脊髓神经

医源性脊髓神经损伤的预防与治疗

Prevention and treatment of iatrogenic injury of spinal nerves

结论胸膜外前路减压与重建手术可解除脊髓的压迫、改善脊髓神经功能、恢复脊柱的序列及重建脊柱的稳定性，且减少胸腔的并发症。

Conclusion Extrapleural anterior decompression and reconstruction could relieve the compression on spinal cord , improve the function of spinal nerves , recover the sequence of spine and reconstruct the stability of spine , and decrease the complications of thoracic cavity .

脊髓神经损伤按ASIA分级：A级6例，B级8例，C级3例。

According to ASIA classification , 6 cases were in Grade A , 8 in Grade B and 3 in Grade C.

周围运动神经传导时间可由电或磁刺激脊髓神经根测量F波的潜伏期而得。

Peripheral motor conduction time by electric or magnetic stimulation of spinal nerve root measurement of F wave latency .

结果：NGF可促进脊髓神经细胞分化、增殖。

RESULTS : NGF enhanced the proliferation and differentiation of cultured embryonic spinal .

目的：观察不同胎龄胎儿脊髓神经丝蛋白（NF）阳性神经元的形态、分布和发育变化，为脊髓-脊髓移植选择适宜的胎龄提供形态学依据。

Objective : To observe the shape and distribution of NF-like neurons in human fetal spinal cord .

受压脊髓神经细胞中ChAT再表达与脊髓神经功能恢复密切相关。

Furthermore , there-expression of ChAT activity in neuronal cells was closely related to the neuronal functional recovery .

HIV-1来源的慢病毒载体转染大鼠脊髓神经干细胞

Lentivirus vector deriving from human immunodeficiency virus-1 transfer to rat spinal cord neural stem cells

体外原代培养大鼠胚胎脊髓神经细胞机械损伤后的凋亡与caspase-3的表达

Apoptosis and caspase-3 expression of primary cultured spinal cord neurons of rat embryo following mechanical injury in vitro

但是IL-1β引起体内脊髓神经细胞凋亡的信号传导途径尚不清楚。

The intracellular signaling events initiated by IL-1 β that lead to apoptotic cell death in vivo , however , are not well understood .

Wnt信号通路对成体小鼠脊髓神经干细胞增殖分化影响的实验研究

Effects of Wnt Signal Pathway on Proliferation and Differentiation of Neural Stem Cells Derived from Adult Mouse Spinal Cord

结论对外伤性C3、4以上不稳并高位脊髓神经损伤手术的高危病例，在保护脊髓治疗的基础上，先处理严重的多发伤及并发症。

Conclusion For patients with upper cervical injuries , the treatment on protecting spinal cord and on administering complications should be given at first .

免疫组化法检测4组大鼠损伤脊髓神经细胞凋亡因子（Bcl-2、Bcl-xl、Bax）的表达；TUNEL标记凋亡细胞。

The expressions of apoptosis factors ( Bcl-2 , Bax , Bcl-xl ) was detected by immunocytochemistry technique , and apoptosis neurons were labeled with TUNEL dyeing .

目的：研究稳恒磁场和HeNe激光对大鼠胚胎脊髓神经细胞增殖分化的影响。

Objective : This paper presents experimental studies in which spinal cord nerve cell of rat embryo were acted by static magnetic field and He-Ne laser at lower power .

结论：NgR在脊髓神经发育的干细胞阶段即开始表达。

Conclusion : NgR is expressed from the stem cell phase during the development of spinal cord .

术后第7天，MP组和对照组脊髓神经功能恢复率分别为75.50%±6.18%和61.02%±6.30%，两组存在显著性差异（P<0.05）。

While compared with the control group , significant improvement of neurological function were found in the MP group on the 7th day after operation ( P < 0.05 ) .

Galectin-3对大鼠脊髓神经干细胞增殖、凋亡及向神经元分化的影响

Effects of galectin-3 on the growth , apoptosis and differentiation into neurons of rats spinal neural progenitor / stem cells

转录调控因子Shh促进胎鼠脊髓神经干细胞体外增殖的效应

Effect on proliferation of neural stem cells in spinal cord of fetal rat in vitro promoted by transcriptional modulator sonic hedgehog

目的研究颌下腺颗粒曲管细胞（GCT细胞）对脊髓神经干细胞（NSCs）增殖分化的影响。

Objective To study the effect of GCT cells of rat submandibular gland on the proliferation and differentiation of NSCs of fetal rat spinal cord .

[结论]一定剂量的NGF能促进大鼠脊髓神经细胞分化和增殖，增强其活性。

[ Conclusion ] Certain concentration of NGF ( 20.0 ~ 80.0 ng / ml ) can promote the differentiation and proliferation of embryonic spinal cord neurons , and enhance the activity of the neurons .

运用脊髓神经功能（BBB评分和Rivlin斜板法）、体感诱发电位、辣根过氧化酶（HRP）示踪、大体观察、组织学及形态计量分析、超微结构变化作为观察指标进行实验观察。

The BBB score and Rivlin plate , SEP , retrograde and label technique of Horseradish Peroxidase , gross observation , histological and morphological analyses were taken as the observed indices .

结论：CT扫描能准确、多方位显示骨折的信息，特别是利用SCT的多方位重建技术，对诊断、判定脊柱失稳，推测脊髓神经损伤情况、精确分类具有十分重要的价值。

Con clu-sion : CT , especially SCT with multiplanar reconstruction , can accurately showed the information of the fracture , moreover it plays an important role in diagnosing , evaluating the stability of vertebral column and assessing the injury of the vertebral nerves .

结论分离自脊髓神经管的细胞具有自我更新能力和多分化潜能（multipotent），是增殖能力很强的神经干细胞；

Conclusion The cells , which express nestin antigen , isolated from neural tube are multipotent and have the ability to self renew , therefore , they are neural stem cells .

结果表明：山羊脊髓神经管闭合以后，其组织发生持续近20d才形成完整的3层同心圆结构；

The results are as the followings : ① Since the nerve duct inosculated , the histogenesis persisted for 20 days and three stratum round structure were constituted .

以脊髓神经功能（CBS计分）、运动诱发电位、辣根过氧化酶（HRP）示踪、组织形态学作为观察指标进行实验观察。

The combined behavioral score ( CBS : A method for examining spinal nerve function ), motive evoked potentials of spine ( MEP ), retrograde and labeling technique of Horseradish Peroxidase ( HRP ), histological morphology were taken as observed indices .

神经电生理检测结果表明OPCs移植治疗组大鼠损伤脊髓神经传导功能的恢复亦明显好于移植对照组和单纯损伤组。

The outcome of electrophysiological experiments indicated that the recovery of the conductive ability of injured spinal cord in the OPCs transplant group was also better than that of the transplant control group and plain injury group .

目的探讨胚胎大鼠脊髓神经干细胞（SNSCs）的分离培养及体外诱导分化的特点和规律。

Objective To investigate the characteristics and rules of cultivation and in vitro inducement ( differentiation ) of spinal cord-derived neural stem cells ( SNSCs ) in vitro from rat 's embryo .

方法显微机械分离孕10～11d胚胎大鼠脊髓神经干细胞，无血清悬浮培养，以复合添加剂N4定向诱导分化，并拍照记录不同时期细胞分化的形态；

Methods SNSCs were isolated mechanically from rat embryonic ( E10 to 11d ) spinal cord under microscope . SNSCs were cultured and maintained in serum-free medium . Directional differentiation from SNSCs to neurocytes was induced by adding N4 supplement .

目的分离培养大鼠胚胎脊髓神经干细胞（SNSCs），研究其体外增殖分化的特点，模拟大鼠胚胎时期脊髓发育的微环境诱导SNSCs向神经细胞定向分化。

Objective To induce directional differentiation from spinal cord-derived neural stem cells ( SNSCs ) into neurocytes in the simulated microenvironment of development of embryonic spinal cord of rat after isolation and culture of SNSCs and to investigate the proliferation and differentiation of SNSCs in vitro .

结论：rHu-EPO能抑制脊髓神经细胞凋亡，对损伤脊髓的神经功能具有保护作用，联合应用rHu-EPO和β-七叶皂甙钠效果更明显。

Conclusion : Apoptosis was an important mode of neuron death after spinal cord injury . rHu-EPO could distinctly advance the reestablishing of the nerve function and protect the nerve tissue . The joined application of rHu-EPO and Sodium β - Aescin is more effective .