解链温度

一个纯合子样品经过PCR扩增之后得到的是同源双链，G：C和A：T之间单个碱基的变化的纯合突变对片段的解链温度有影响。

A homozygous sample after PCR amplification , contains a homoduplex , G : C and A : T single base changes have an impact in the melting temperature of the fragment .

以l-色氨酸为原料，设计并合成b-咔啉-3-甲酰胺类化合物，利用粘度滴定、Tm（解链温度）测定及紫外光谱滴定法以及微量量热等方法测定其与CT-DNA的作用。

The interactions of these compounds with CT-DNA were determined by the viscometric titration assay and Tm ( melting temperature ) value assay . Binding strengths were evaluated by spectrophotometric titration experiment and microcalorimetric measurement .

它们DNA的解链温度相同。

The thermal denaturation temperature of their DNAs was same .

双链DNA解链温度的特征自组织预测方法（英文）

Prediction melting temperature of DNA duplex by self-organization feature map ( SOFM )

双链DNA解链温度的最小二乘支持向量机预测方法

Prediction of melting temperature of DNA duplex by least squares support vector machine

基于BP神经网络的DNA解链温度预测模型

Predicting Melting Temperature of DNA Duplex by BP Neural Network

根据解链温度计算出噬菌体DNA的G+C含量在42.9与72.1mol%之间。

The G + C content of the DNAs ranged between 42.9 and 72.1 mol % as estimated from their melting temperatures .

利用分形和分维理论研究DNA的复制机理并计算BDNA共聚物中氢键的解链温度。

The copy principle of DNA is studied by using fractal and fractal dimension theory and hydrogen-bond melting temperature in BDNA copolymers calculated .

当片段一定的情况下，单个碱基的差异能反映在解链温度上，表现在熔解曲线的差异。

A single base change can be reflected in the melting temperature difference , the performance difference in the melting curve .

当片段在一定条件下，单个碱基的变化对片段的解链温度有影响，通过高分辨率和灵敏度的检测技术可以检测出来。

When the fragment under certain conditions , single base changes have an impact in the melting temperature of the fragment , and it can be detected through the high resolution and sensitivity detection technology .

方法根据DNA二聚体的热力学性质，在DNA阵列芯片上测定其解链曲线，用数学的方法获得其解链温度（Tm）值，再根据Tm值进行SNP分析。

METHODS Melting curves were determined in the DNA microarray , in terms of the thermodynamic traits of DNA duplexes , and the Tm values was calculated for SNP analysis .