负染

用电镜负染技术观察角蛋白细丝并测定其直径为80～100A。

Under negative staining electron microscopy , the keratin was a filament with a diameter of 80-100A .

电镜负染检查可见直径为40￣60nm有突起的圆形或近圆形的病毒颗粒，病毒在蔗糖中的浮密度为1.13￣1.14g/cm3。与牛病毒性腹泻-粘膜病病毒颗粒相似。

By negative staining electron microscope , virions were roughly round and approximately 40 ~ 60 nm in diameter with peplomers . Density is 1.13 ~ 1.14g/cm3 . The hemagglutination reaction test proved that this virus had no any reaction to erythrocyte of chook , rabbit , pig and sheep .

方法采用旋转蒸发法将PS与SOD结合，制备PSSOD脂质体，电镜负染法观察PSSOD脂质体的形态，测量其大小。

Methods PS-SOD liposome was made through rotation-evaporation method . Its morphology and diameter were observed and measured with an electronic microscope .

本文获得的清晰DEV负染超微结构照片，为该病毒结构生物学的研究提供了重要依据。

In this paper , some clear DEV negatively stained pictures were presented and this provided important information for DEV structure biology .

本文用免疫电镜负染法对病毒性乙型肝炎患者13份粪便进行观察，检出了HBsAg，与实验室检查结果相符。

By immune electron microscopic observation on 13 fecal specimens ( negative stain ) from patients with hepatitis B virus , HBsAg was found in some of the specimens .

电镜负染、切片观察，酶切、PCR扩增及测序鉴定的结果表明表达犬瘟热核蛋白基因的重组犬2型腺病毒构建成功，表达的核蛋白分子量为58kDa。

We demonstrated that a recombinant canine adenovirus type-2 expressing nucleocapsid protein of canine distemper virus was successfully constructed and observed by electron microscopy , enzyme digestion , PCR and sequencing . The molecular mass of nucleocapsid protein was 58 kDa .

电镜负染（TEM）结果，亲本菌株可以表达完整的菌毛，突变株△pilM菌毛缺失。

Negative staining electron microscopy ( TEM ) results , the parental strains can express a complete pili , did not express mutant △ pilM .

负染观察到的病毒核衣壳呈直杆状，但长度和直径相差较大，最长的病毒超过600nm。

Nucleocapsid , observed by negative stain , is rod shaped , and its length and width are various .

结果表明：病料接种MDCK细胞产生明显的细胞病变（CPE），电镜负染观察接毒细胞培养物见有典型的犬瘟热病毒粒子。

The results showed that the infected material could cause cytopathogenic effect ( CPE ) in MDCK cells . Typical virions of CDV were observed in culture fluids with negative staining electron microscope .

电镜观察负染标本病毒形态近似圆形和六角形，无胞膜，直径约30nm。

The morphology of the virus was similar to round and hexagonal without capsule , diameter 30 nm by negative stain under electron microscope .

将得到的病毒提纯液经磷钨酸负染后进行电镜观察，结果表明在电镜下可看到大量线状病毒粒子的存在，大小约为800nm。

Virus particles in the extracted solution were observed under a transmission electron microscopy after negative staining with phosphotungstic acid . The results showed that there are a lot of filamentous particles about 800 nm long .

分离物上清液负染电镜观察到冠状病毒。

Coronavirus was observed in the culture supernatant by negative-stain electron microscopy .

扫描及负染电镜在兔脑炎原虫病诊断中的应用

Use of the Scanning and Negative Staining Electron Microscopy in Diagnosing Encephalitozoonosis

兔骨骼肌肌球蛋白分子形成凝胶过程的负染观察

Observation of Gel Formation from Rabbit Striated Muscle Myosin Molecules

方法负染法和超薄切片法进行检测。

Methods Negative staining and ultrathin sections were used for electron microscopic examination .

质量评价研究中，建立了灯盏花素脂质体质量评价方法，对脂质体质量进行全面评价，用负染法、透射电镜法对脂质体形态进行表征，脂质体颗粒圆整、均匀。

In the research on quality evaluation , we established an overall quality standard of breviscapine liposome .

用超薄切片和原生质体负染的方法，在电镜下观察到洋葱鳞茎内表皮细胞周质微管的存在。

Cortical microtubules were observed in intraepidermal cells of onion bulb with thin section and negative staining of protoplasts .

应用免疫电镜和负染法检测马铃薯试管苗中的微量病毒。

Immune electron-microscope and negative stained methods were used to examine the trace virus in tube plants of potato .

负染病毒粒子与超薄切片大小形态相同。

The morphology and size of virion in negative staining were the same as the observation in ultrathin sections .

本文通过负染电镜技术和单颗粒分析的方法首次对发菜藻胆体进行了深入研究。

In this work the phycobilisomes from Nostoc flagelliforme were first carefully studied by electron microscopy and single particle analysis .

将纯化前后的病毒悬液用磷钨酸负染后直接透射电镜观察，可见到完整的病毒粒子及其干净的背景；

Background of electron micrograph of the purified virus is clean and the complete structure of virus particles can be clearly seen ;

该病毒的完整粒体有囊膜，负染观察可见病毒粒体有尾。

The intact virion is enclosed with two-layer-envelope , and it has a tail when it is observed by electronic microscope in negative stain .

从表现为叶斑驳、花碎色症状的香石竹病株上获得一病毒分离物，电镜负染观察到直径为28～33nm的球状粒子。

A virus isolated from carnation with mosaic leaves and broken flowers . Through TEM , the virus particle was spherical of about 28-33 nm in diameter .

通过用铁浸染色法对细菌群体结构定量化研究，用糖包被负染法对群体结构内部结构观察，对粘附在组织表面细菌数量的测定，反映结构与粘附力的关系。

The colonial structure was observed by glycocalyx negative stain . The adhesive bacteria on tissues were estimated . These reflected the relation between colonial structure and adhesion .

病毒粒子经1%醋酸铀负染后镜检，病毒粒子分为有囊膜和完全或部分失去囊膜两种。

The viral particles , negatively stained with 1 % uranium acetate , observed under transmission microscope , appeared to be enveloped or unenveloped because of the envelope being damaged .

本研究应用离子捕获电镜技术和直接负染电镜技术比较检测了临床上疑似为犬传染性肝炎和犬细小病毒性肠炎的犬送检样品。

We have conducted a comparison for detecting pathogen of doubtable infectious canine hepatitis and canine viral enteritis in clinical investigation with ion catch electron microscopy and direct negative staining electron microscopy .

病毒粒体为正20面体，直径约25nm.用差速离心等方法提纯的病毒，经醋酸铀负染，粒子为20面体，直径为28nm。

The purified virions of the isolate TN were obtained by differential centrifugation and stained negatively with 2 % uranyl acetate . The virus particle morphology was spherical about 28 nm in diameter .