酶标记法

结果与结论：Taq酶标记法和大肠杆菌Klenow片段随机引物延伸标记法同样有较好的标记效果，且随机引物或特定引物作为延伸引物均可以合成足够有效的探针。

Results and conclusion : Taq DNA Polymerase labeling method is as efficient as Klenow fragment random primer DNA labeling system .

方法选取膀胱占位的患者48例，健康志愿者20例作对照，留取患者术前及对照组人群的晨尿1次，应用免疫酶标记法检测尿中NMP22的数值，并进行分析比较。

Methods NMP22 level of morning urinary from 48 cases of bladder tumor and 20 healthy volunteers were measured by ELISA and analyzed .

原位末端转移酶标记法（TUNEL）检测心肌细胞凋亡；

The incidence of cardiomyocyte apoptosis was evaluated by the TdT-mediated dUTP nick end labeling ( TUNEL ) staining and the expression of Bcl-2 and Bax were evaluated by Flow Cytometry ( FCM ) .

方法：采用p53原位杂交、原位脱氧核糖核酸末端转移酶标记法（TUNEL）和免疫组织化学技术对56例胃癌进行检测。

Methods : Fifty six cases of gastric cancer were studied with immunohistochemical staining ( S P methed ) and for apoptosis by in situ TUNEL , p53 genes were detected by digoxigenin labeling in situ hybridization technique .

测量大鼠心脏重量（HW）和体重（BW）并计算心脏重量与体重之比（HW/BW）；原位末端酶标记法（TUNEL）检测各组大鼠心肌细胞凋亡情况；

Heart weight ( HW ) and body weight ( BW ) of each rat were measured to ratio HW and BW , The apoptosis of myocardial cell was evaluated by TUNEL .

方法：应用免疫酶标记法（ELISA）检测58例尿路上皮癌、32例良性泌尿系疾病、18例其他恶性肿瘤和20例健康志愿者尿中NMP22水平。

Methods : Urinary NMP22 values were determined for 58 patients with urothelial transitional cell carcinomas ( TCC ), 32 with benign urological diseases , 18 with other cancers and 20 healthy volunteers by an enzyme-linked immunosorbent assay ( ELISA );

应用辣根过氧化物酶标记法，发现猫空肠回肠交感节后纤维来自腹腔神经节和肠系膜前神经节以及双侧T9～L5交感干神经节。

The innervation of the jejunum and ileum in the cat were studied by the HRP method . We found that the sympathetic postganglionic fibers arise from the coeliac ganglion , superior mesenteric ganglion and bilateral T9 ￣ L5 ganglia of the sympathetic trunk .

末端转移酶标记法原位检测大脑神经细胞凋亡状况。

Apoptosis was observed using terminal transferase marking method .

用辣根过氧化物酶标记法对鸡盲肠传入神经元分布的研究

The Location of the Afferent Neurons of Caecum in Fowl & A study on the HRP Tracing Method

应用酶联免疫标记法（ELISA）测定血浆BNP浓度。

Labeling enzyme-linked immunosorbent ( ELISA ) was used to measure the plasma BNP concentration .

方法：采用酶标记免疫吸附法测定女性乳腺癌、乳腺良性病变患者血清中LEP、IGF-1的浓度，并与其他临床资料进行对比分析。

METHODS : The concentrations of LEP the IGF-1 were detected in female breast cancer and benign breast lesions through the enzyme-labeled immunosorbent assay ( ELISA ) and the results were compared with other clinical data of the patients .

酶标记免疫测定法；酶免疫测定；酶免疫分析法免疫印迹法、酶联免疫法及放射免疫法对胰岛自身抗体检测的比较

Enzyme immunoassay ( EIA ) Comparison between Assay of Immunoblotting Test , ELISA and RIA in Detection for Islet Autoantibodies

传染性非典型肺炎冠状病毒特异性IgG抗体检查应用酶标记免疫吸附测定法进行检测。

The severe acute respiratory syndrome coronary virus specific immunoglobulin G antibody was detected with enzyme-labelling immunosorbent assay .

运用原位末端脱氧核苷转换酶（TdT）标记法（TUNEL）和DNA阶梯法检测细胞凋亡。

In situ terminal deoxynucleotidyl transferase ( TdT ) labeling ( TUNEL ) and DNA ladder were applied to detect apoptosis .

用辣根过氧化物酶标记抗体Fab染色法在光镜及电镜下检测大肠癌的CEA标志

Detection of CEA Marker of Colon-Rectal Carcinoma under Light and Electron Microscopes Using Horseradish Peroxidase Labeled Anti-CEA Fab Antibody

我们认为酶标记抗原直接ELISA法为HFRS的早期诊断提供了更为特异、简便快速的新方法。

We think that direct ELISA that uses HRP labeled antigen provides a more specific , simpler and faster method for early di agnosis of HFRS .

方法用辣根过氧化物酶（HRP）标记法观察猫动眼神经横断并分别采用导管套接和神经桥接修复后，其动眼神经运动核内神经元的变化。

Methods Horseradish peroxidase ( HRP ) retrograde mark method was used to observe the changes of the motoneurons in oculomotor nucleus after the conduit repair of cat oculomotor nerves .

但是传统的酶检测方法，如放射性标记、酶标记法等，往往操作繁杂，这在一定程度上限制了酶分析的研究。

But the traditional methods to detect enzyme , such as the radioactive label and the enzyme label , generally have complex operations that has limited the advanced research in enzyme in some degree .