重组体

对于重组体dna问题，我们需要有一个全面的估价，而且最好由联邦政府主办。

We need a comprehensive assessment of the recombinant DNA issue , preferably under the auspices of the federal government .

重组体DNA也是这类问题的一个极好的例子。

Recombinant DNA is also an excellent example of this problem .

目的：从DNA重组体中快速鉴定出阳性克隆。

Objective : To identify positive clones from DNA recombinants rapidly .

介绍一种PCR鉴定重组体DNA插入方向及转染的方法

Identification of transfected gene and orientation of inserts in recombinants by PCR

狼疮鼠淋巴细胞IgG2aVH区反义RNA重组体的构建及鉴定

Construction of recombinant antisense RNA IgG2aV_H region of murine systemic lupus erythematous

通过酶切、PCR及测序鉴定各重组体。

The recombinants were analyzed and identified by restriction enzyme , PCR and sequencing .

HBVs基因重组体的构建及免疫小鼠的实验

Construction of HBV S gene recombinant and its immunity induced in mice

人扁桃体淋巴细胞cDNA质粒重组体的构建

The establishment of plasmid recombinant of the human tonsil lymphocytes cDNA

谷胱甘肽S－转移酶pi基因与逆转录病毒载体的重组体构建

Construction of Recombinant Glutathione S-transferase pi cDNA and Retrovirus Vector

结论：人扁桃体淋巴细胞cDNA质粒重组体构建成功。

Conclusion : Plasmid recombinant of the human tonsil lymphocytes cDNA has been successfully established .

HCV重组体转染细胞系的研究

Study of The HCV Recombinant Transfection Cell Lines

发卡样CTGF特异性RNA干扰重组体的构建及筛选

Cloning and screening of the recombinant plasmids affecting gene CTGF by RNA interference

从该F2群体中，共筛选到44个重组体。

A total of 44 recombinants were selected in this population .

构建人CD23及其反义RNA的逆转录病毒表达重组体

Construction of retrovirus recombinants for expressing human CD23 and its antisense RNA

CTGF靶向RNA干扰重组体的构建及序列分析

Construction and sequence analysis of connective tissue growth factor targeted recombinant plasmids by RNA interference

一株呼吸道合胞病毒融合糖蛋白F和EGFP共表达的非复制型腺病毒重组体的构建、鉴定及表达

Construction and Identification of Replication-defective Recombinant Adenoviruses Which Co-express the Fusion Protein of RSV and EGFP

HSV-tk基因逆转录病毒重组体的构建与DNA序列分析

Construction of the Retroviral Recombinant Plasmid Vector Encoding Thymidine Kinase Gene of the Herpes Simplex Virus and DNA Sequencing

人TIMP-2基因逆转录病毒重组体的构建及表达

The Construction and Expression of Retroviral Recombinant Containing Human TIMP-2 Gene

采用电转染方法将重组体导人HL-60细胞中；

The recombinant plasmid was transfected into the cell line HL - 60 by electroporation .

不同因素HCV结构区基因重组体诱发小鼠产生抗体的影响

The Effect of Different Administrations of pCD-HCV 1 on Antibody Responses in Mice Immunized by Gene Recombinant of HCV Structure Region

结果表明本实验成功构建了汉滩病毒包膜糖蛋白G2的重组体。

The results showed that this experiment successfully constructed Hantaan virus en-membraned glucoprotein G2 recombinant .

共表达禽流感病毒H5、H7亚型HA基因DNA重组体构建及小鼠免疫试验研究

Construction of co-express HA Gene of AIV H5 、 H7 Subtype DNA Recombination and the Immune Study of Mouse

拟南芥同源转换盒基因A21反义RNA基因重组体的构建及转化

Construction and Transformation of an Antisense RNA Gene Recombinant of Arabidopsis Homeobox Gene A21

方法RT－PCR扩增瘦素受体长胞内段cDNA，将扩增产物插入真核表达质粒，构建重组体。

Methods Amplifying fragment of leptin receptor long intracellular domain cDNA by RT-PCR , then inserting the fragment into eukaryotic plasmid to construct recombinant ;

结果病毒重组体的病毒滴度在乒乓超感染后有明显提高，最高病毒滴度约为1×106CFU／ml；

Results The virus titers of both recombinants increased obviously after ping pong procedures .

壳聚糖纳米基因载体介导IL-10和HGF基因重组体在大鼠活体肝移植中的研究

Study on Recombinant Interleukin 10 and Hepatocyte Growth Factor By Chitosan Nanoparticle Gene Vector in Liver Transplantation

c-jun反义基因重组体转染对缺氧复合烧伤血清刺激下大鼠心肌细胞的保护作用

Protective effects of c-jun anti-sense gene recombinant transfection on rat cardiomyocytes inflicted by hypoxia and burn serum

【结论】本实验成功地扩增出完整EBVDNA多聚酶基因，并构建了高效表达重组体，为进一步在体外表达、制备EBVDNA多聚酶蛋白奠定了基础。

The establishment of the recombinant efficient expression vector carrying EBV DNA polymerase gene has provided a base for producing EBV DNA polymerase protein in vitro .

反义PARP基因真核表达重组体的构建及其HeLa转化细胞系的建立

Construction of antisense PARP cDNA recombinants and establishment of the HeLa transformant after antisense plasmid introducing

目的：探讨重组体人α-2b干扰素联合乙型肝炎疫苗、左旋咪唑涂布剂治疗慢性乙型肝炎的临床疗效。方法：176例慢性乙型肝炎患者随机分为两组：A组和B组，每组88例。

Objective : To evaluate the mechanism and clinical effect of Anfuron ( Recombinant Human Interferon α - 2b Injection ) combined with hepatitis B vaccine and liniment levamisole for treating chronic hepatitis B ( CHB ) .