金丝桃素

HPLC法灵敏、准确、重现性好，可用于金丝桃素注射液含量的测定。

HPLC method was established for the determination of hypericin injection .

贯叶连翘提取物中金丝桃素的初步HPLC测定

Preliminary Determination of Hypericin in Hypericum perforatum Extract by HPLC

金丝桃素对C6胶质瘤细胞周期变化的研究

The effects of cell cycle by photodynamic hypericin induced C_6 glioma apoptosis

贯叶连翘薄膜片中金丝桃素和金丝桃素类化合物的HPLC和UV测定

Determination of Hypericin and Hypericins in Hypericum perforatum Film-coated Tablets by HPLC and UV

金丝桃素分子结构及其与HIV病毒蛋白酶作用的分子动力学研究

Studies on Molecular Structure of Hypericin and Its Interactions with HIV-1 Protease by Molecular Modeling

建立乙肝清胶囊中金丝桃素的HPLC含量测定方法。

The research aimed to establish HPLC method for determination of hypericin in Yiganqing capsule .

建立了元宝草中主要活性成分金丝桃素的HPLC定量分析方法。本方法有良好的重现性及精密性，为金丝桃属植物的金丝桃素质量控制提供了检测方法。

The method developed for quantitative analysis of hypericin in H. sampsonii by HPLC has good reproducibility and accuracy .

RP-HPLC法测定贯叶连翘片剂中有效成分金丝桃素和金丝桃苷的含量

Determination of Effective Compounds Hypericin and Hyperoside in Hypericum perforatum Tablets by RP-HPLC

MTT结果显示光活化金丝桃素呈剂量依赖性抑制喉癌细胞的生长。

MTT assay showed that hypericin associated with light irradiation inhibited growth of laryngeal cell with dose dependence manner .

金丝桃素蛋白络合物体外抗H5N1亚型禽流感病毒的活性研究

Study on the antiviral activity of hypericin protein-bound complex on H5N1 subtype of AIV in vitro

用鸡胚法进行了金丝桃素蛋白络合物抗H5N1亚型禽流感病毒活性研究。

The antiviral activity of hypericin protein-bound complex on H5N1 subtype of AIV was investigated by using chicken embryos .

光激活金丝桃素对大鼠C6胶质瘤抑制作用及其对bFGF和bFGF-R及MVD的影响

Therapeutic function and affection on bFGF , bFGF-R and MVD about photo-activated hypericin to rat C6 glioma

用PMA预先处理细胞24h后，再给予金丝桃素刺激发现荧光强度并没有进一步下降。

However , after stimulation with a 24 h preincubation of PMA and 5 concentrations of hypericin , no further decrease was observed .

金丝桃素+光照组中Flt1蛋白表达和MVD明显低于非光照组及对照组（P<0.05），呈剂量依赖关系。

Their expression of HY + light group is lower than that of the group of control and of no-light . The same as MVD .

方法应用流式细胞术（FCM）、MTT法和3H-TdR掺入法观察光活化的金丝桃素对C6胶质瘤细胞株的细胞周期动力学变化的影响。

Methods The cell biological changes of glioma cell strain ( C 6 ) were observed by Flow cytometry technique ( FCM ), MTT colorimetric assay and 3 H-TdR method .

紫外-可见光谱研究了光、温度、pH、Na2SO3及抗坏血酸对金丝桃素稳定性的影响。

The effects of light , temperature , pH , vitamin C and sodium sulfite on the stability of hypericin has been studied by using UV-Vis spectrum .

本文以A类动物烈性传染病之首的口蹄疫病毒（foot-and-mouthdiseasevirus，FMDV）为模型，进行金丝桃素新制剂抗RNA病毒的生物试验研究。

The experiment uses the FMDV ( short for foot-and-mouth disease virus , and FMD is one of the OIE list A diseases ) as the model to study the Hypericin New preparation effects of anti-RNA virus .

建立了贯叶连翘中金丝桃素的测定方法，利用HPLC法和分光光度法测定贯叶连翘浸膏中金丝桃素的含量，检测波长为590nm。

The use of HPLC and Spectrophotometric Determination was to get the content of hypericin in the Hypericum perforatum extract . And detection wavelength was 590 nm .

其主要药用成分为金丝桃素类物质，具有抗抑郁、抑制中枢神经、增强免疫功能和显著的抗DNA、RNA病毒作用，可用于艾滋病的治疗。

As for medicine , its main composition is hypericin and its derivatives which be used for antidepressant , depressing the central nervous system , and has immunotropic activity and marked anti-DNA virus and anti-RNA virus , therefore , it used as to cure AIDS in last decade .

两种洗脱液经HPD-100和D-101树脂的进一步分离纯化后，得到金丝桃素含量为40%和黄酮类含量为80%的两种产品。（3）金丝桃素HPLC分析和制备方法的研究。

40 % hypericin and 80 % flavone was got after these two desorption solutions were purified further by HPD-100 and D-101 . ( 3 ) HPLC analysis and preparation of hypericin .

方法：1.利用超临界C02流体萃取技术提取金丝桃素（贯叶连翘提取物的主要活性成分），并用高效液相法测定其含量。

Use supercritical CO2 fluid extraction technology to extract hypericin ( the main active ingredient of TEHP ) and determine its content by HPLC .

目的：通过评价金丝桃素、盐酸赖氨酸体外抗疱疹病毒活性，为进一步研究疱疹后遗神经痛（PHN）以及继发抑郁的治疗提供更多理论支持。

AIM : To provide more theoretic support for further studies on the treatment of post herpetic neuralgia ( PHN ) and depression by evaluating the effects of hypericin and lysine hydrochloride against herpes virus in vitro .

方法：用生长抑制试验和流式细胞技术观察给予金丝桃素后PC3M细胞的增殖、细胞周期和凋亡的变化。

Methods Growth inhibition test and flow cytometry were used to examine the changes of proliferation , cell cycle and apoptosis .

并在此基础上摸索出金丝桃素的HPLC制备方法，制备色谱条件是：流动相，甲醇-乙酸乙酯-0.1M磷酸二氢纳（615：235：150）；

Temperature of chromatogram columniation , room temperature . The HPLC preparation method was concluded on the base of the analysis method . The chromatogram condition of preparation shows : Flowing phase , methanol-ethyl acetate -0.1M NaH2PO4 ( 615:235:150 );

光活化的金丝桃素诱导胶质瘤细胞调亡的实验研究

Experimental study of glioma cells induced by light-induced photoactivation of hypericin

贯叶金丝桃素及其衍生物的合成与神经药理学研究进展

Advance in chemical and neuropharmacological studies on hyperforin and its derivatives

金丝桃素玻璃体腔注射能显著降低兔慢性高眼压模型视网膜谷氨酸含量。

Intravitreal injection of hypericin can decrease the retinal glutamate content .

使用金丝桃素联合用药治疗猪高热病

Using hypericin as add-on drug treating pigs ' high fever disease

金丝桃素新制剂的毒理学研究

Study on the Toxicology of the New Preparations of Hypericin

正交试验法研究金丝桃素提取工艺

Studies on process for extraction of hypericin by orthogonal experiments