银耳

银耳多糖对小鼠IL－2、IL－6、TNF－α活性及其mRNA表达的影响

Effects of Tremella Polysaccharides on il-2 , il-6 , tnf - α activity and their mRNA expressions in mice

银耳多糖（TP）是从福建产银耳子实体中提取的含蛋白多糖。

Tremella polysaccharide ( TP ) is an important component isolated from Tremella fuciformis Berk .

结果表明，银耳交配信息素对热、pH、盐离子、蛋白酶均不稳定。

It was proved that the mating pheromone was unstable to heat , pH , salty ion and proteinase .

应用Matlab软件进行多项式曲线拟合，表明二次多项式能够有效地表达各干燥条件下的银耳干燥动力学曲线。

Quadratic polynomial could express drying kinetics curves of every drying condition by using Matlab software for polynomial curves fitting .

磁性测试结果表明，在室温下这种银耳状的Co（0.85）Se具有顺磁性。

And magnetism test results show that the tremelliform Co ( 0.85 ) Se possesses paramagnetic property at room temperature .

使用HPLC法测定人参皂苷Rb1、阿霉素、异硫氰酸荧光素的载药量，采用分光光度计法测定银耳多糖的载药量。

Determinated drug loading of ginsenosides Rb1 , adriamycin , fluorescein isocyanate by HPLC and Tremella polysaccharide by Spectrophotometer .

高赖氨酸蛋白基因（lys）转化银耳

Lysine-rich protein gene ( lys ) transforming Tremella fuciformis

结论IL2激活脾LAK细胞是有效的抗肝癌细胞，IL2与银耳多糖在活化脾细胞提高抗肿瘤方面具有协同作用。

Conclusion IL-2 activated LAK cells are effective anti-hepatocarcinoma cells .

AchE活性以取食平菇的最高，取食银耳的活力最低，取食木耳的居中。

The activity of AChE was highest when larvae fed on Pleurotus ostreatus , and then Auricularia auricular , the lowest was Tremella fuciformis .

结论：银耳多糖是通过调控细胞周期负调控因子p21的转录和表达、抗氧化及增强免疫功能而抗衰老的。

Conclusion : TP have anti-aging effects by regulating transcription and expression of cell cycle negative regulator p21 , anti-oxidation and strengthen immunity .

并可以装载人参皂苷Rb1、阿霉素、异硫氰酸荧光素、银耳多糖。

And this method can load ginsenoside Rb1 , adriamycin , fluorescein isothiocyanate and Tremella polysaccharide .

结论：银耳孢子多糖对小鼠U14宫颈癌、H22肝癌和S180肉瘤，均有较好的抑制作用。

CONCLUSION Polysaccharide from fermented Tremella fuciformis have better inhibition effects on U14 cervical cancer , H22 hepatoma and S180 sarcoma in mice .

银耳多糖协同IL2实验组织较单纯的IL2实验组表现的更为明显。

The presentation on Tremella Polysaccharide and IL-2 experiment group was more significant than that in simple IL-2 group .

本试验结果表明银耳芽孢发酵液（BN-901）对小鼠移植性肿瘤肉瘤S180有明显的抑制作用；

Cultures of Tremella fuciformis Beik ( BN-901 ) have been demonstrated to inhibit sarcoma 180 in mice .

结果银耳多糖（Tp）口服后仅有微量（约0.4%）通过胃肠道入血，被器官所吸收。

RESULT Tremella polysaccharide ( T p ), isolated from Tremella fuciformis Berk , was absorbed into blood about 0.4 % only and then was distributed into organs and tissues after it was administered orally .

银耳（Tremella）是一种寄生于枯木上的高级食用真菌，具有独特的营养、美容及药用价值，受到广大消费者的青睐。

White fungus which parasitizes on withered wood , is an advanced edible fungus , and has special nutrition , cosmetology as well as medicinal value .

体外实验用基因重组技术将p21基因整合到人胚肺成纤维细胞基因组中，建立细胞衰老模型，并用银耳多糖对此进行干预；

P21 gene were integrated into the genome DNA of HELF cells by DNA recombination technique to make cell-aging model in the experiments in vitro . This cell-aging model was interfered by TP .

体内实验：银耳多糖可以下调小鼠p21的转录与表达，抗氧化，增加小鼠皮肤中羟脯氨酸含量及增强小鼠的免疫功能。

Experiments in vivo : TP can down-regulate transcription and expression of p21 gene , have the activity of anti-oxidation , increase contents of hydroxyproline in the skin and strengthen immunity in mice .

载人参皂苷Rb1和银耳多糖的人血白蛋白微粒载药量较高，载阿霉素和异硫氰酸荧光素的人血白蛋白微粒载药量较低。

Ginsenoside Rb1 and Tremella polysaccharides human serum albumin particles have higher drug loading , and adriamycin and fluorescein isothiocyanate human serum albumin particles have lower drug loading .

在正交试验的基础上，以多糖含量和感官评价为考察指标确定了银耳茶的最佳配方为银耳58.3%、枸杞8.4%、绿茶33.3%，最佳生产工艺条件为温度90℃，提取5h。

With the polysaccharide content and sensory evaluation as the evaluation criterion , the optimal recipe is 58.3 % tremella , 8.4 % medlar and 33.3 % green tea .

测定了银耳的β-半乳糖苷酶（LACZ）和β-葡萄糖苷酸酶（GUS）的活性，确定GUS作为银耳遗传转化的报告基因。

Based on results of the activity of P - galactosidase ( lac Z ) and P - glucosidase ( GUS ) of T. fuciformis tested , GUS gene was established as report gene for genetic transformation .

银耳（TremellafuciformisBerk.）又称白木耳，是一种已大面积人工栽培、被人们普遍接受的滋补食品。

Tremella fuciformis Berk , was call as White Jelly Fungus or White Wood Ear . It was cultivated in large scales and was known as tonic food or health food .

目的采用高效凝胶渗透色谱法（HPGPC）对银耳多糖质量控制进行了方法学研究。

Quality control of Tremella polysaccharides ( TP ) has been studied with high-performance gel permeation chromatography ( HPGPC ) .

以银耳（Tremellafuciformis）芽孢为实验材料，正交实验研究超声波处理时间、间歇时间及功率对转化率的影响。

Yeast-like conidia of Tremella fuciformis was used as material to study ultrasonic mediated transformation . The effect of ultrasonic treatment time , intermission and power on transformation rate was studied with orthogonal experiment .

经红外光谱、超速离心及电镜观测可知：银耳多糖铁（Ⅲ）配合物是以β-（FeOOH）。

The investigation by infrared spectra , ultracentrifugation and electron microscopy showed that structure of ITC was an interracial compex with β - ( FeOOH ) .

经试验发现，旋转蒸发及吸附剂活性碳、D4020、AB-8、聚酰胺、离子交换剂D61均不适合用于银耳交配信息素的分离。

It was proved that the adsorbent including activated carbon , D4020 , AB-8 , polyamide and the ion exchange resin D61 had no adsorbent or exchange function to the mating pheromone .

本研究在探索银耳遗传转化体系的基础上，构建了高赖氨酸蛋白基因表达载体，利用限制性内切酶介导整合法（REMI），首次把高赖氨酸蛋白基因导入银耳芽孢，获得了转基因菌株。

In the study , on the basis of studying the system of genetic transformation of Tremella fuciformis , a lysine-rich protein gene expression vector was constructed , and then transferred into Tremella fuciformis spores with Restriction Enzyme-Mediated DNA Integration ( REMI ) .

银耳菌丝原生质体分离和再生研究

Studies on Isolation and Regeneration of Protoplast from Tremella Fuciformis Mycelium

银耳枣汁酸奶的研制

Study on technology of mixed fermented yoghurt with jujube and tremella

液体深层发酵条件对银耳菌丝体酸性多糖富集的影响

Effects of Submerged Fermentation on Acidic Polysaccharides in Tremella fuciformis Mycelia