鸟嘌呤核苷酸

巯基嘌呤甲基转移酶活性和硫鸟嘌呤核苷酸浓度检测在6-MP个体化化疗中的意义

Significance of TPMT activity and TGNs level detection for individualizing 6-mercaptopurine chemotherapy

MPA的主要作用机理是抑制次黄嘌呤单核苷酸脱氢酶（IMPDH），该酶是鸟嘌呤核苷酸从头合成途径的限速酶。IMPDH受抑导致鸟嘌呤核苷酸生成减少，进而DNA、RNA合成受阻。

The main mechanism of MPA is that it inhibits IMPDH which is the rate-limiting enzyme in de novo synthesis of guanosine nucleotides .

证据表明，①在小牛睾丸细胞膜G蛋白上存在两种类型鸟嘌呤核苷酸结合位点（下简称GTP结合位点），高亲和性低容量结合位点及低亲和性高容量结合位点；

It was shown by evidence that ( 1 ) there were two classes of guanine nucleotide binding sites on the Gp of calf testis membranes , high affinity ( with low capacity ) binding sites and low affinity ( with high capacity ) binding sites ;

目的探讨巯基嘌呤甲基转移酶（TPMT）活性、基因型和硫鸟嘌呤核苷酸（TGNs）浓度检测对6巯基嘌呤（6MP）化疗个体化的意义。

Objective To evaluate the activity and genotype of thiopurine methyltransferase ( TPMT ) and the concentration of thioguanine nucleotides ( TGNs ) as parameters for individualizing mercaptopurine ( 6-MP ) therapy .

硫鸟嘌呤核苷酸浓度检测在儿童急性淋巴细胞白血病6-巯嘌呤个体化疗中的意义

Significance of TGNs detection for individualizing 6-MP chemotherapy in children with acute lymphoblastic leukemia

该酶所催化的反应对维持细胞内腺嘌呤和鸟嘌呤核苷酸的平衡发挥着重要作用。

It plays an important role in maintaining the intracellular balance of A and G nucleotides .

目的：建立一种快速、灵敏、高效的方法，检测儿童急性淋巴细胞白血病红细胞中硫鸟嘌呤核苷酸浓度。

Objective To establish a HPLC method to measure thioguanine nucleotides concentration in children with acute lymphoblastic leukemia .

为检测婴幼儿配方奶粉中胞嘧啶核苷酸、尿嘧啶核苷酸、腺嘌呤核苷酸、鸟嘌呤核苷酸、次黄嘌呤核苷酸5种核苷酸的含量，建立了奶粉中5种核苷酸的紫外检测器液相色谱法。

For the determination of 5 nucleotides in infant formula milk powder , the HPLC-UV absorbance detector method was established .

结论：在优化条件下，本方法测定硫鸟嘌呤核苷酸快速、准确、灵敏度高。

CONCLUSION : Under optimized conditions , HPLC method for the determination of thioguanosine is fast , accurate and sensitive .

目的研究我国汉族人纤溶酶原激活物抑制物1基因启动子区675位4G5G（单鸟嘌呤核苷酸插入缺失）基因多态性与心肌梗死和脑梗死的等位基因特异性的相关性。

Objective To investigate the relationship between the 4G / 5G gene polymorphism of the plasminogen activator inhibitor 1 ( PAI 1 ) and myocardial and cerebrovascular infarctions .