乙酰化酶

去乙酰化酶是临床上常见的关键调节细胞和器官流程，包括代谢、细胞分裂和老化。

Sirtuins are key regulators of clinically important cellular and organismal processes including metabolism , cell division and aging .

靶向DNA甲基化和组蛋白去乙酰化酶的几个表观药物已经在临床试验中被测试。

Several epigenetic drugs targeting DNA methylation and histone deacetylation enzymes have been tested in clinical trials .

这些结果显示，即使组蛋白去乙酰化酶可以作为治疗AD的潜在靶点，但是持续的用药还是必需的。

These results indicate that although histone deacetylase can be served as a potential target for treating AD , continuous drug administration may be required .

[目的]建立以氯霉素乙酰化酶（CAT）为报告基因的雄激素受体（AR）报告基因试验。

[ Objective ] To develop an androgen receptor ( AR ) reporter gene assay in which the reporter gene is chloramphenicol acetyltransferase ( CAT ) .

组蛋白去乙酰化酶抑制剂诱导HeLa细胞p21~（WAF1/CIP1）表达的分子机制研究

Molecular mechanism of p21 ~ ( WAF1 / CIP1 ) expression induced by histone deacetylase inhibitors in HeLa cell line

本文用放射免疫测定法测定了吗啡对家兔、大鼠低位脑干胆碱乙酰化酶（ChAT）活力的影响。

The effect of morphine on the activity of choline acetylase ( ChAT ) in the lower brain stem of rats and rabbits was determined by radioimmunoassay .

结果表明：组蛋白去乙酰化酶抑制剂TSA可有效的诱导MOLT-4细胞发生G2／M阻滞和凋亡，并且呈现明显的剂量效应关系和时间效应关系；

The results showed that TSA could effectively induce G2 / M arrest and apoptosis of MOLT-4 cells .

Raf激酶抑制蛋白介导的信号通路对肝癌侵袭转移的抑制作用组蛋白去乙酰化酶抑制剂及甲基转移酶抑制剂协同抑制造血干细胞分化

Inhibition Effects of Signal Transduction Mediated by Raf Kinase Inhibitor Protein on Invasion and Metastasis of Hepatocellular Carcinoma

目的：探讨组蛋白去乙酰化酶（HDAC）抑制剂对系统性红斑狼疮（SLE）患者外周血全血培养细胞中IL-10和IL-12分泌水平的影响。

Objective : To explore the effect of histone deacetylase inhibitors on the excretion levels of IL-10 and IL-12 in patients with SLE .

目的：改进组蛋白去乙酰化酶抑制剂SAHA的合成工艺。

Objective : To improve the synthetic procedure of the histone deacetylase ( HDAC ) inhibitor SAHA .

目的：研究组蛋白去乙酰化酶抑制剂曲古抑菌素A（TSA）对前列腺癌LNCaP细胞抑制作用的细胞信号机制。

AIM : To investigate the molecular mechanisms underlying the antitumor effect of trichostatin A ( TSA ) on LNCaP prostate cancer cells .

提示姜黄素抑制组蛋白乙酰化酶P300可能是其抗癌的分子机制之一。

Our study may imply that curcumin can treat cancer by inhibiting the expression of P300 , which may be one of its molecular mechanism of anticancer .

结论组蛋白去乙酰化酶引起的组蛋白H4去乙酰化与干扰素γ激活IP-10基因有关。

Conclusion The histone H4 deacetylation at the ISRE site is related with the activation of IP-10 by IFN-gamma .

丙戊酸（valproicacid，VPA）作为一种组蛋白去乙酰化酶抑制剂，能特异性抑制组蛋白去乙酰化酶的活性，提高细胞内组蛋白乙酰化水平，激活基因的表达。

Valproic Acid ( VPA ), a histone deacetylase inhibitor , could specifically inhibit the histone deacetylase activity , increase histone acetylation , and active gene expression in somatic cells .

多种具有组蛋白乙酰化酶（HAT）和组蛋白去乙酰化酶（HDAC）活性的调节因子，通过对组蛋白乙酰化过程的调节，在基因表达调控中发挥着重要作用。

Recent studies showed that some transcriptional regulators with histone acetyltransferase ( HAT ) and deacetylase ( HDAC ) activities may play a causative role in regulating gene expression .

有研究表明，去乙酰化酶（HDAC）抑制剂通过阻断HDAC活性可以介导多种细胞的分化。

Studies have shown that , HDAC inhibitors can mediate the differentiation of several cells through inhibited the activity of HDAC .

目的建立简化的一步等位基因特异扩增法（ASA），研究中国人N-乙酰化酶（NAT2）多态性等位基因的分布。

Objective To establish a simplified one-step allele specific PCR amplification ( ASA ) for study the distribution of alleles of NAT2 polymorphism .

目的探讨组蛋白脱乙酰化酶抑制剂苯丁酸钠（PB）体外阻断AML1ETO的生物学功能，消除AML1ETO的转录抑制，诱导Kasumi1细胞生长抑制、分化和凋亡作用。

Objective To explore the blockade effect of phenylbutyrate ( PB ), a histone deacetylase inhibitor , on the in vitro biological function of AML1 / ETO to reverse its transcription repression and induce Kasumi-1 cells to differentiate and apoptosis .

本实验通过一种组蛋白去乙酰化酶抑制剂TSA来诱导不同时期胚胎核心组蛋白高乙酰化，观察其对早期胚胎发育能力及相关基因mRNA表达水平变化的影响。

Trichostatin A ( TSA ), one of the inhibitors of HDACs , was used to determine the effects of core histone hyperacetylation on development ability and expression level of relational gene mRNA in different developmental embryos .

组蛋白乙酰化酶（HATs）和组蛋白去乙酰化酶（HDACs）是组蛋白乙酰化的关键酶，其决定着组蛋白乙酰化的程度，参与了肿瘤异常基因表达。

Histone acetyltransferases and histone deacetylases which are histone acetylation key enzyme determined the level of histone acetylation and participated the expression of tumor abnormal gene .

另外，组蛋白去乙酰化酶抑制剂TSA诱导组蛋白超乙酰化，促进Sp1与mda-7启动子结合，进而激活mda-7的表达。

Moreover , HDAC inhibitor TSA induced histone hyperacetylation and stimulated Sp1 binding to mda-7 promoter , which in turn enhanced the expression of mda-7 .

已有报道证实组蛋白去乙酰化酶抑制剂（HDACi）可以引起哺乳动物基因组的去甲基化，这支持了DNA甲基化和组蛋白修饰可以相互作用调节基因的表达的观点。

Histone deacetylase inhibitor ( HDACi ) has been shown to demethylate mammalian genome , which further strengthens the concept that DNA methylation and histone modifications interact in regulation of gene expression .

组蛋白去乙酰化酶（HDACs）通过调节转录因子的活性等影响成骨发育。

Histone deacetylases are enzymes ( HDACs ) that can regulate the activity of transcription factors and affect bone development .

本研究运用RNA干扰技术对小鼠HDAC2进行了干扰，通过抑制组蛋白去乙酰化酶的表达提高乙酰化修饰水平，以探索表观遗传修饰影响哺乳动物胚胎早期发育的可能机制。

This study was designed to improve the level of acetylation by knock down the expression of histone deacetylase expression with RNA interference technology . A possible mechanism was provided for improving epigenetic modifications and exploring the early development of mammalian embryos . 1 .

因此，本试验以TSA为代表药物，研究组蛋白去乙酰化酶抑制剂对猪卵巢颗粒细胞的作用，并就其机理作初步探讨，以为临床用药提供一定的参考。

Therefore , this study aims to investigate the effect of histone deacetylase inhibitors on porcine granulosa cells and its preliminary mechanism . All the results can provide a reference for clinical use .

组蛋白去乙酰化酶（HDAC）对染色质分布和基因调节起着重要的作用，也是治疗癌症和其它疾病的新靶点。

Histone deacetylase ( HDAC ) greatly affects the chromatin topology and gene expression , and HDAC can be a new strategy in human cancer or tumour therapy .

目的：本研究探讨组蛋白去乙酰化酶抑制剂TrichostatinA（TSA）联合化疗药物顺铂（DDP）处理人卵巢癌顺铂耐药株C13的协同效应。

Objective : To investigate the synergistic effects of a histone deacetylase inhibitor , trichostatin A ( TSA ), combined with cisplatin ( DDP ) on cisplatin-resistant ovarian epithelial cancer cell line C13 .

分别以不同浓度的去乙酰化酶抑制剂valproicacid（VPA）对普氏原羚-牛异种重构胚处理不同时间，观察克隆胚胎的发育。

In this study , five experiments were designed to examine the developmental potential of interspecific gazelle-cattle reconstructed embryos . ( 1 ) The gazelle-bovine reconstructed embryos were treated with a deacetylase inhibitor valproic acid ( VPA ) for different time and at different concentrations .

目的：观察组蛋白去乙酰化酶（HDAC）抑制剂曲古霉素A（TSA）对体外培养膀胱癌细胞生长情况及相关基因表达的影响，并探讨其可能的作用机制。

Objective : To investigate the influence of trichostatin A ( TSA ), a histone deacetylase ( HDAC ) inhibitor , on the growth of human bladder cancer cells and on the expression of related genes , and to explore the mechanism involved .

本研究通过观察组氨酸去乙酰化酶（HistoneDeacetylase，HDAC）抑制剂TrichostatinA（TSA）对卵巢癌细胞株A2780CAR表达水平的影响，探讨HDAC抑制剂在腺病毒载体基因治疗中应用的可能性。

This study was to evaluate enhancive effect of trichostatin A ( TSA ), a histone deacetylase ( HDAC ) inhibitor , on the transfection efficiency of adenovirus in ovarian carcinoma cell line A2780 , and explore its possible application to adenovirus-based gene therapy .