乙酰化修饰

在本文中我们探讨了可逆的组蛋白乙酰化修饰对IL-5基因转录转录的影响，并对其可能的机制做了深入的研究。

In this study , we explored the possible regulatory mechanisms of reversible histone acetylation on human IL-5 gene transcription .

组蛋白乙酰化修饰是表观遗传学中的重要组成部分，组蛋白乙酰化酶（HAT）和组蛋白去乙酰化酶（HDAC）之间的动态平衡控制着染色质结构和基因表达。

Histone acetylation is an important part of epigenetics . The dynamic equilibrium between Histone acetyltransferase ( HAT ) and histone deacetylase ( HDAC ) can control the chromatin structure and gene expression .

DNA甲基化及组蛋白乙酰化修饰是表观遗传修饰的主要方式。

DNA methylation and histone acetylating modification are the main forms of epigenetic modification .

p300/HDAC介导的可逆的乙酰化修饰参与Th1细胞因子IL-12和IL-18基因转录水平的调控

Reversible Histone Acetylation / deacetylation Modification by p300 / HDACs is Involved in the Regulation of IL-12 / IL-18 Transcriptional Activity

精子发生过程中组蛋白H4乙酰化修饰

The acetylation of histone H4 during spermatogenesis

组蛋白乙酰化修饰对IEC-6恶性转化细胞细胞周期和p53、p21~（WAF1）基因表达的调控

Regulation of histone acetylation on cell cycle and p53 , p21 ~ ( WAF1 ) genes expression in malignant transformation of IEC-6

以大肠杆菌表达的Tα1，与天然的Tα1具有相同的氨基酸组成，唯一不同的是N端缺少乙酰化修饰。

So the downstream purification was simplified , and the production cost was decreased . Both and natural T α 1 have the same amino acid composition , but T α 1 expressed from E coli is without acetylation in N - terminus .

因此，我们得出结论组蛋白乙酰化修饰在调控mda-7表达中发挥重要作用，并且转录因子Sp1参与此过程。

Therefore , we conclude that histone acetylation modification is an insignificant factor in regulation of mda-7 , and the transcription factor Sp1 participates in this process .

采用经典的吡啶-乙酸酐法首次对人参皂甙Rb1进行乙酰化修饰，从反应物比例、反应时间等方面进行优化，确定最佳反应条件为：使用2当量的乙酰化试剂室温反应2h。

The acetylation of Ginsenoside Rb1 was carried out using the classical method – pyridine and acetic anhydride . The following optimum reaction conditions were determined : the acetylation reagent , 2 equ ; reaction time , 2 h ; reaction temperature , the room temperature .

可逆的组蛋白乙酰化修饰对人白细胞介素5基因转录的调控作用及机制研究

The Regulatory Mechanisms of Reversible Histone Acetylation on Human IL-5 Gene Transcription

组蛋白乙酰化修饰在基因表达调控中的作用机制

Role of histone acetylation modification in posttranscriptional gene expression

Tα1是一个由28个氨基酸残基组成的多肽，其N-末端具有乙酰化修饰。

T α 1 is a 28-amino acid peptide with an N-terminal acetylation .

褐变菜籽分离蛋白质酶水解及乙酰化修饰研究

Study on Hydrolyzing and Modifying Brown Rapeseed Isolated Protein by Enzymes and Acetic Anhydride

组蛋白的乙酰化修饰在转录调控中的作用近年来成为表观遗传学的研究热点。

Histone acetylation in transcriptional regulation has become hot spot in epigenetics in recent years .

组蛋白乙酰化修饰调控果蝇热休克基因表达和寿命的分子机制研究

Molecular Mechanisms of Histone Acetylation Modification in Regulation of Hsp Gene Expression and Lifespan in Drosophila Melanogaster

尤其是乙酰化修饰作用在染色质结构的改变和转录调控方面发挥着重要的作用。

In particular , acetylation modification play an important role in chromatin structure changes and transcriptional regulation .

研究发现神经系统中与学习和记忆相关的蛋白质，其基因水平的调控主要跟组蛋白乙酰化修饰有关。

Of the epigenetic modifications identified so far in the nervous system , histone acetylation has been unequivocally associated with facilitating learning and memory .

本文以多头绒泡菌为实验材料，利用其天然同步化的优点，从新的角度研究了组蛋白乙酰化修饰对真核细胞周期调控的作用机制。

In this thesis , we studied the mechanisms of histone acetylation in cell cycle regulation in Physarum polycephalum , a naturally synchronized slime mold .

组蛋白乙酰化修饰与基因的表达调控相关，参与了各种肺部疾病的病变过程。

The acetylation of histone is related to gene expression and regulation , which takes part in the pathological courses of all kinds of lung diseases .

乙酰化修饰作为一种关键的翻译后修饰被广泛的研究，但是在此以前的研究几乎都集中于组蛋白和核内蛋白上，对于核外蛋白的乙酰化研究进展很慢。

As a key post-translational modification , protein acetylation was studied comprehensively , but the majority of acetylation studies have been focused on histones and nuclear transcription regulators .

已有研究表明组蛋白乙酰化修饰异常引发人类疾病主要是由于重塑复合物中的关键蛋白发生突变，影响基因的正常表达而引发人类疾病。

Studies have shown that the abnormity of histone acetylation may lead to human diseases , which mainly due to remodeling the key protein complex and affecting the normal expression of genes .

软件分析发现nNOS1f启动子内存在一些潜在的受乙酰化修饰的转录因子结合位点；萤光素酶报告基因检测在启动子内发现两个正调控区和两个负调控区。

Software analysis suggested a number of putative responsive elements of transcription factors that are subject to acetylation within nNOS If promoter . Luciferase assay indicated two positive and two negative regulatory regions . 4 .

另外，根据近年来表观遗传学研究进展，组蛋白的泛素化，甲基化，乙酰化等修饰作用共同形成了组蛋白密码，在基因转录调控中起关键性作用。

Moreover , based on the epigenetic research progress in recent years ," histone code " that is including modification of histone ubiquitination , methylation , acetylation , etc. , plays a crucial role in the regulation of gene transcription .

在真核生物中，组蛋白是染色质基本结构一核小体中的重要组成部分，其N末端氨基酸残基可发生乙酰化等共价修饰。

Covalent modifications , such as acetylation occur in the N-terminal amino acids of histones .

结果：这些化学修饰至少包括：①半胱氨酸残基丙烯酰胺加合物生成或乙酰基烷基化修饰，相应的肽段质量数分别增加71.04和57.02；

Results : These modifications at least included : ① acrylamide adduct formation ( 71.04 Da added ) or alkylation of cysteine residues by iodoacetamide ( 57.02 Da added );

目前发现有如下几种表观遗传分子机制：DNA甲基化，RNA干扰，组蛋白乙酰化和组蛋白修饰。

There are several types of epigenetic inheritance systems methylation of DNA , RNA interference , histone acetylation and protein modification .

其他的工作集中在组蛋白乙酰化，这种化学修饰可以使DNA从组蛋白缠绕中解离出来，产生基因活性。

Other work has focused on histone acetylation , a chemical modification that unwinds DNA from protein spools called histones , thereby enabling gene activity .

果蝇中染色体乙酰化与去乙酰化修饰及其与热休克基因表达的关系

Roles of Chromosome Acetylation and Deacetylation in Hsp Gene Expression in Drosophila

组蛋白乙酰化/去乙酰化修饰染色质在基因表达中起关键作用。

Modifications of chromatin structure by acetylation / deacetylation of the histone proteins play a central role in the regulation of gene expression .

本研究运用RNA干扰技术对小鼠HDAC2进行了干扰，通过抑制组蛋白去乙酰化酶的表达提高乙酰化修饰水平，以探索表观遗传修饰影响哺乳动物胚胎早期发育的可能机制。

This study was designed to improve the level of acetylation by knock down the expression of histone deacetylase expression with RNA interference technology . A possible mechanism was provided for improving epigenetic modifications and exploring the early development of mammalian embryos . 1 .