供试品溶液

照光学异构体检查项下方法试验，供试品溶液主峰的保留时间应与对照品溶液左氧氟沙星峰的保留时间一致。

Proceed as directed in the Optical Isomer , the retention time of the major peak of the test solution corresponds to that of the reference solution of Levofloxacin .

供试品溶液的pH对细菌内毒素检查法结果的影响

Effect of pH Value on Bacterial Endotoxin Test

目的确定细菌内毒素检查法中，供试品溶液的pH对细菌内毒素检查法检测结果的影响。

OBJECTIVE To confirm the effect of pH value of sample solution on bacterial endotoxin test .

供试品溶液在2h内稳定；

The testing solution for assay was stable within 2 hours .

采用旋涡混合器以水为溶剂制备供试品溶液，以大口径HP-快速GC残留溶剂柱为分离柱，FID为检测器，用外标法进行定量。

Water as solvent , sample solution was prepared by using vortex mixer . The determination was performed on a wide bore HP-fast GC residual solvent column with FID detector . The external standard method was used for quantitative analysis .

标准对照溶液：制备方法同供试品溶液，使用规定体积的标准铅溶液（10ppmPb）替代供试品进行试验。

Reference solution ( standard ) . Prepare as described for the test solution , using the prescribed volume of lead standard solution ( 10 ppm Pb ) R instead of the substance to be examined .

方法将相同浓度的供试品溶液与对照品溶液点于同一硅胶GF254薄层板上，采用薄层色谱法，观察各主斑点的位置、大小及颜色。

Methods Drop the sample liquor and the contrast liquor with the same consistence on the one siliceous GF 254 thin-layer vitreous board and use thin-layer chromatography to observe the place of the every main fleck , size and color .

方法采用聚酰胺柱纯化去杂的方法制备供试品溶液。

Methods The sample was prepared through polyamide column .

样品经二甲亚砜超声提取，离心，过滤得供试品溶液。

Samples was made by extracting with DMSO , ultrasonic , centrifugation and filtration products .

用水制备供试品溶液，加入氯化钠消除电离干扰，将供试品溶液喷入乙炔-空气火焰，用标准曲线法测定氯化钾的含量。

The sample solutions were prepared with water and sodium chloride was added to eliminate ionization interference .

方法不同提取溶剂、不同提取方法，较为的供试品溶液制备方法。

Method Different extraction solvents and methods have been screened to get the most efficient way for sample preparation .

方法：将供试品溶液的浓度由10mg/ml降为4mg/ml及通过加热处理。

Method : Change its concentration from 10mg / ml to 4mg / roland deal with by heating in water .

根据研究对象的溶解性能，分别采用微波消解法和直接溶解法制备供试品溶液。

Microwave digestion and direct dissolution were respectively used to prepare sample solutions according to the solubility properties of gliclazide and iohexol .

结果：薄层色谱鉴别中，本品供试品溶液与对照品溶液相应位置具相同颜色的荧光斑点，空白对照无干扰。

Result : In the identified process , the testing solution showed the same light spot with control solution , the blank had no interference .

结论：硫酸头孢匹罗中的杂质是对细菌内毒素检查产生干扰的主要因素，而调节其供试品溶液的酸度可以有效地排除杂质所带来的干扰。

Conclusions : Related impurity of cefpirome sulfate is the mostly effect factor , but the interference of related impurity can be eliminated from adjusting its acidity .

通过对其提取方法、溶剂倍数、提取次数、提取时间等条件进行考察，确定了该供试品溶液的制备方法。

The method to prepare solution of product is determined after search of its extraction method , solvent multiples , the number of extraction , and extraction time .

对检测过程中的影响因素进行了考察，实验发现，由于原料与片剂的供试品溶液制备方法不同，尤其是片剂的水溶步骤，是导致活力偏低的主要原因。

The influential factors during the test are investigated . We find that different methods of preparing solution for the materials and tablets , especially the procedure to dissolve tablets , are the main reasons leading to the low vitality .