化学分化

诱导细胞形态通过倒置显微镜观察，并通过免疫细胞化学检测分化细胞表达Insulin和Nestin。

Differentiated cells were observed under inverse microscope , insulin and nestin expressed in differentiated cells were detected with immunocytochemistry .

免疫细胞化学染色分化细胞形态观察。

The morphological changes of differentiation cells by immunocytochemical staining .

在培养基中加入血清后，免疫细胞化学检测分化后细胞包括微管相关蛋白阳性、胶质纤维酸性蛋白阳性和2,3-环核苷酸磷酸二酯酶阳性细胞，巢蛋白表达均为阴性。

After adding serum to medium and differentiation of immunocytochemical detection , the cells included positive MAP-2 , positive GFAP and positive 2,3 - CNP , while the expression of the nidogen was negative .

来自基础教育课改区的部分教师反映，在实施了新课程后，九年级学生化学学习分化显著，甚至在一些学校出现了学生化学学习两极分化的结果。

According to the responses of the teachers from the basic education curriculum reform district , chemistry learning result division is remarkable in grade nine after having implemented the new course and even the chemistry learning results polarization in some schools appears .

方法：本实验采用纹状体组织块与胚胎干细胞联合培养的方法，通过形态学观察和酪氨酸羟化酶（TH）免疫细胞化学染色对分化细胞进行鉴定。

Methods : Embryonic stem cell s were co-cultured with striatum explants from newborn mouse brain . The differentiated cells were evaluated by morphological observation and TH immunocytochemical staining method .

采用细胞化学染色、分化抗原检测、细胞周期测定、联合NBT与MTT还原试验计算A（NBT）/A（MTT）值等方法分析细胞的分化或凋亡特点。

The characteristics of cell differentiation or apoptosis were analyzed by cytochemical staining , differentiation antigen detection , cell cycle assay and A ( NBT ) / A ( MMT ) value determination .

利用免疫组织化学方法鉴定分化后的细胞类型。

The cell types were identified by immunochemistry method .

免疫细胞化学染色检测诱导分化前后细胞Nestin、PDX1、胰岛素、生长抑素的表达；

The expression of Nestin , PDX 1 , insulin , somatostatin in cells before and after differentiation were detected using immunocytochemistry method .

免疫细胞化学鉴定不同诱导分化阶段细胞的白蛋白（Alb）、甲胎蛋白（AFP）、细胞角蛋白-18（CK-18）等肝细胞特征的表型变化并计数阳性细胞比率。

The expressions of albumin ( Alb ), alpha fetoprotein ( AFP ), cytokeratin-18 ( CK-18 ), and cytokeratin-19 ( CK-19 ) were detected with immunocytochemistry to identify the characteristics of the differentiated cells at different times and the ratio of the positive cells was determined .

方法常规方法培养脂肪组织来源的多能干细胞，细胞免疫化学方法对未分化的细胞进行鉴定。

Methods Adipose tissue - derived multipotent stem cells were cultured routinely .

5周后观测大体形态和组织学形态、Ⅰ和Ⅱ型胶原免疫组织化学表达，测量分化组织的细胞活力。

Gross appearance , histological section , immunohistochemistry and cell vitality were investigated after 5 weeks .

在诱导第7，14，21天分别行甲苯胺蓝染色、免疫细胞化学染色检测其分化结果。

At day7,14 and21 after induction respectively , Toluidine blue staining and immunocytochemical staining were performed to detect differentiation .

对初中生来说，化学符号学习是化学学习的关键阶段，教学实践表明，化学符号学习是初中化学学习的一个重难点，特别是学生化学学习的分化点。

For junior middle school students , chemical symbol learning is the key stage in learning chemistry , and the teaching practice shows that chemical symbol learning is a key and difficult point of chemistry learning in middle high school , especially point of differentiation of students ' chemical learning .