密度梯度离心

方法：分别应用上游法、Percoll密度梯度离心法及双曲管优选法处理精液，观察优选前后精子染色体畸变率、精子性染色体比例及超微结构相对正常的精子百分率。

Methods : The selection techniques of swim-up , Percoll density gradient centrifugation and doule-flection tube were used to observe sperm chromosome abnormality rate , sex chromosomal ratio and the percentage of spermatozoa with relatively intact head ultrastructure .

快速密度梯度离心法纯化质粒DNA

Purification of plasmid DNA by rapid density gradient centrifugation

方法细胞：采用密度梯度离心法，分离人脐血单个核细胞（MononuclearCells，MNCs）。分组：分为3组。

Method Human umbilical blood mononuclear cells ( MNCs ) were separated with density gradient centrifugation .

密度梯度离心法获得大鼠MSCs，传代培养并扩增。

MSCs were obtained from SD rats using the method of density gradient centrifugation culture .

结果1密度梯度离心法成功分离纯化大鼠MSCs。

Rat MSCs had been separated and purified by density gradient centrifugation successfully . 2 .

结论1.经观察细胞形态和细胞表面标志物鉴定，采用密度梯度离心结合贴壁法可在体外成功分离、培养MSCs。

MSCs can be isolated by density gradient centrifugation and adherent culture in vitro . 2 .

结果：1.体外培养MSCs的形态特征经密度梯度离心的MSCs形态比较均一。

Morphological characteristics of MSCs in vitro by density gradient centrifugation of MSCs relatively uniform shape .

结果：Percoll密度梯度离心法培养可获得均一的MSCs；

RESULTS : Homogeneous MSCs were obtained by percoll density gradient centrifugation .

结论：用密度梯度离心和贴壁培养法可以分离并培养得到bMSCs。

Conclusion : bMSCs can be obtained from bone marrow by the density gradient centrifugation method .

方法：选用54只雄性SD大鼠，密度梯度离心法分离骨髓间充质干细胞（MSCs）。

METHODS : Bone marrow MSCs were obtained by density gradient centrifugation from the tibiae of 54 male SD rats .

结论利用Percoll密度梯度离心法结合贴壁法可以获得比较纯的MSCs；

Conclusion High pured MSCs can be obtained by Percoll gradient centrifuging and adherent method .

Percoll密度梯度离心分离不同发育阶段生精细胞的方法

Separation of Mouse Spermatogenic Cells by Centrifugation in Percoll Density Gradients

通过密度梯度离心与红细胞裂解后联合贴壁培养法可获取较为纯化的MSCs。

MSCs were purified by the method of split red blood cell , combining gradient density centrifugation and adhesion separation .

结论联合采用密度梯度离心和贴壁筛选的方法易于分离、纯化获得纯度较高的MSCs。

Conclusion : MSCs is easy to be isolated and purified by combined using density gradient centrifugation and plastic adherence .

脊髓灰质炎病毒（Poliovirus）的Percoll等密度梯度离心分离

Poliovirus Separation by Centrifugation in Percoll Isopycnic Gradients

所有实验组病例和对照组均采取4毫升静脉血，肝素抗凝，密度梯度离心法分离PBMCs。

PBMCs were separated from 4 ml heparinized blood by density gradient centrifugation .

Percoll密度梯度离心法分离大鼠肺Ⅱ型上皮细胞

Isolation of Rat Type ⅱ Alveolar Epithelial Cells by Discontinuous Percoll Gradient Centrifugation

采用Percoll非连续密度梯度离心法纯化大鼠乳鼠心室肌细胞

Enrichment of cardiomyocytes from neonatal rat ventricular tissues by discontinuous percoll gradient centrifugation

研究方法：采用Percoll密度梯度离心法分离大鼠BMSCs，对所获得的细胞进行体外培养及鉴定。

Methods BMSCs were isolated by percoll solution from rat bone marrow sample , cultured and identified .

本文采用区带密度梯度离心，国内首次分离得到黄牛纯化的高密度脂蛋白（HDL）。

The bovine high density lipoprotein ( HDL ) was first isolated and purified in our country with zonal gradient ultracentrifugation .

方法采用机械分离、酶消化和不连续密度梯度离心法分离提取TIL前体细胞；

Methods TIL were obtained through mechanical separation , enzyme digestion and discontinuous density gradient centrifugation .

应用Percoll非连续密度梯度离心法富集人前列腺上皮细胞

Enrichment of Epithelial Cells from Human Benign Prostatic Hyperplasia by Discontinuous Percoll Gradient Centrifugation

Percoll等密度梯度离心法分离利什曼原虫细胞核

Isolation and purification of nuclei of Leishmania gerbilli by Percoll isopycnic density gradients centrifugation

用改良胶原酶消化技术和聚蔗糖密度梯度离心方法将大鼠胰岛从胰腺分离，按Sun等的方法用海藻酸和聚赖氨酸等予以微囊化。

Rat pancreatic islets were microencapsulated with , sodium alginate and poly-lysine , according to Sun 's method after being isolated from pancreatic tissue using modified collagenase digestion and Ficoll density gradient separation .

方法胶原酶灌注结合Percoll密度梯度离心加选择性贴壁的方法分离SEC；

Methods SEC were isolated by the combination of collagenase perfusion , percoll gradient isopycnic centrifugation and selective adherence ;

方法：抽取SD大鼠的骨髓，经密度梯度离心分离，联合贴壁筛选法获取纯化的骨髓间充质干细胞，以流式细胞仪鉴定间充质干细胞表面标志。

MSCs were isolated by gradient density centrifugation and plastic adherence and then purified , The surface markers were identified with flow cytometry after amplification in vitro .

分别采用Ficoll密度梯度离心、自然沉降和明胶沉降三种方法分离MNC。

Density gradient centrifugation ( Ficoll ), gelatin sedimentation and natural sedimentation were compared .

结果：1、利用密度梯度离心法和贴壁法均可以成功在体外分离、培养MSC。

Results : 1 、 MSC can be successfully isolated and developed in vitro with density gradientcentrifugation method and sticking wall method .

结果用甘油密度梯度离心，可从病毒粒子中分离由NP、3P和RNA组成的RNP。

Results The RNA-dependent RNA polymerase of influenza virus was isolated from virus particles by stepwise centrifugation in different salts .

方法：将抽取的兔子骨髓用密度梯度离心和贴壁培养法分离bMSCs，在体外进行细胞的原代和传代培养，观察细胞的生长情况。

Methods : bMSCs were extracted from the bone marrow in rabbits by density gradient centrifugation method and cultured in vitro regularly .