碱性蛋白酶

稳定剂对2709碱性蛋白酶作用的探讨

Studies on the Effection of Stabilizer on 2709 Alkali Protease

研究了在蒸煮的大豆培养基上其产蛋白酶的特性，结果表明：该菌株具有一定的产蛋白酶的能力，产生的主要是碱性蛋白酶。

Its characteristics of producing protease were studied . The results showed that strain could produce protease , mainly alkali protease .

利用枯草杆菌碱性蛋白酶E基因的信号顺序构建分泌表达载体

Construction of Secretion Vectors Using Signal Sequence of Bacillus subtilis Alkaline Protease E Gene

Bacilluslicheniformis6816碱性蛋白酶基因在E.coli中的克隆和表达

Cloning and expression of the gene encoding alkaline protease from Bacillus licheniformis in E.coli

TritonX-100-无机盐双水相体系的相平衡模型及碱性蛋白酶在该体系中的分配系数模型

Modeling of Triton X-100-salt aqueous two-phase systems and distribution of alkaline protease

小麦赤霉病禾谷镰刀菌（Fusariumgraminearum）产碱性蛋白酶的发酵条件

Fermentation Conditions for Alkaline-protease Production of Fusarium graminearum Causing Fusarium Head Blight in Wheat

最后，将首次提出的用加压CO2从混合溶剂中沉淀蛋白质的新方法，运用于纤维素酶分离纯化和酸性蛋白酶、碱性蛋白酶去除杂蛋白过程。

Finally , the new process with precipitating of proteins from mixed solvent using compressed carbon dioxide was applied to purification of cellulase , acid proteinase and basic proteinase .

产低温碱性蛋白酶海洋适冷菌SY的筛选

Screening of a Marine Psychrophilic Bacterium ( SY ) Producing Low-Temperature Alkaline Protease

研究了戊二醛的浓度、给酶量、固定化温度、时间、pH对固定碱性蛋白酶的影响。

The factors involving with the activity of immobilized alkaline protease , such as the concentration of glutaraldehyde , amount of alkaline protease , reaction temperature , time and pH , were studied .

研究了大豆分离蛋白（SPI）的细菌碱性蛋白酶水解产物的聚集行为。

The objective of this study was to investigate the aggregation of soy protein isolates ( SPI ) induced by Protex .

利用FPLC技术建立重组碱性蛋白酶的快速纯化方案

Construction of Fast Purification Method of Recombinant Alkaline Protease with FPLC

本实验以枯草杆菌A4－3为出发菌株，发酵产生碱性蛋白酶，其野生型菌株产酶为2412u／ml。

Bacillus subtilis A4-3 was used as the starting strain in the experiment . Through fermentation , the production of alkaline proteinase was 2412 u / ml.

目的：为进一步确认海洋假单胞菌碱性蛋白酶（MPAP）的纤溶性质，并观察其抗栓作用。

Objective To further study the characteristics of fibrinolytic activity and the thrombolytic effect of MPAP .

采用十二烷基酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）方法，分析了几种商品蛋白酶（包括枯草杆菌蛋白酶、胰蛋白酶、胰凝乳蛋白酶、木瓜蛋白酶和细菌碱性蛋白酶）对大豆分离蛋白（SPI）的降解模式。

SPI hydrolysates induced by several commercial proteinases ( subtilisin protease , chymotrypsin , trypsin , papain , and Protex ) were analyzed by the SDS-PAGE .

鲢鱼鱼肉蛋白的碱性蛋白酶酶解物在一定条件下与葡萄糖反应可以提高其清除DPPH自由基能力。

Thus , it is efficient to improve scavenging DPPH radical activity of the hydrolysates of silver carp protein by maillard reaction .

将1L发酵液经过盐析、透析后，得到少量黄褐色的碱性蛋白酶。

A few brown alkaline protease is obtained by salting and dialyzing fermentation liquid .

由于碱性蛋白酶mRNA翻译起始区的复合二级结构和低起始强度以及依赖链霉素突变核糖体高级结构的改变，使依赖链霉素突变核糖体不能翻译碱性蛋白酶mRNA。

These results suggest that Str-D ribosomes could not translate apr mRNA because of the secondary structure complex , low initiation strengh of apr mRNA , and alteration of the higher order structure of the Str-D ribosomes .

方法根据6种单酶的水解产物对ACE抑制作用的测定结果，确定了两种酶即菠萝蛋白酶和alcalase2.4L碱性蛋白酶组成复合水解酶。

Methods Based on the inhibitory activities of six kinds of single enzymic hydrolysates to angiotensin converting enzyme ( ACE ), bromelain and alcalase ( 2.4L ) were selected as combined-enzyme .

在碱性蛋白酶mRNA翻译起始区有一个复合二级结构，用体外突变方法破坏其中一个，翻译效率提高8.2倍。

There is a secondary structure complex at the translation initiation region of apr mRNA . When one of the secondary structure was destroyed by site directed mutagenesis , the translation efficiency was enhanced by 7.3 to 9.1 folds .

利用快速蛋白液相层析（FPLC）技术，建立了快速高效纯化碱性蛋白酶的方案。

The fast purification method of recombinant protease was conducted with FPLC ( Fast Protein Liquid Chromatography ) .

菌株15E所分泌的碱性蛋白酶的分子量为49.0kDa，等电点为9.3，米氏常数Km为5.6×10~（-3）g／mL。

Strain 15E could secrete alkaline protease , whose molecular weight was 49.0 kDa , isoelectric point was 9.3 and Km value was 5.6 × 10 ~ ( - 3 ) g / mL.

耐盐高温碱性蛋白酶产生菌株SD-142的脱毛条件研究

Application of alkaline protease producing Bacillus sp . SD-142 in unhairing process

目的观察海洋假单胞菌碱性蛋白酶（MPAP）的溶栓和抑制血栓形成的作用，研究其对纤溶、凝血和血小板聚集功能的影响。

ObjectiverTo observe the thrombolysis and anti-thrombosis effects of MPAP ; To study the actions of MPAP on blood coagulation , platelet aggregation and fibrinolytic activity .

高温碱性蛋白酶产生菌株SD-142的分离、鉴定及发酵产物性质的初步研究

Isolation 、 Identification and Products Characterization of Strain SD-142 Producing Thermostable Alkaline Protease

黄海黄杆菌YS-9412-130低温碱性蛋白酶性质鉴定

Study on properties of low-temperature protease of Flavobacterium YS-9412-130

从连云港海域、港口、远洋捕捞船及鱼市等地采集的海水和各类海鱼、贝类等样品中分离得到一株产碱性蛋白酶的海洋适冷细菌CHS。

CHS , a marine psychrophilic bacterium that produces low-temperature alkaline protease , has been isolated from the seawater , and fish and seashell obtained from Lianyungang Harbor , fishing boats and market places .

结果表明，在吡啶溶剂中9种商业酶以枯草杆菌碱性蛋白酶催化Michael加成效果最好，并在50℃表现了最好的催化活性；

The effect of organic medium and enzyme resource on enzymatic tandem acylation / Michael addition and one-pot synthesis ; 8 enzymes have catalytic activity in 9 commercial enzyme , the catalytic activity of alkaline protease from Bacillus subtilis in anhydrous pyridine was the highest .

一株产低温碱性蛋白酶嗜冷海洋细菌YS-9412-130的分离和培养条件研究

Study on isolation and culture condition of one marine bacterium YS 9412 130 producing low temperature alkaline protease

粗酶液的最适作用温度为40℃，最适pH值为10，在pH9～12内稳定，是一碱性蛋白酶。

The optimum pH and temperature for the protease activity production are pH 10.0 and 40 ℃ . The enzyme is alkaline protease . Some other properties of the strain and the protease were discussed in detail .

利用APTES对磁性介孔硅载体表面进行修饰，然后用偶联剂PDC将其与碱性蛋白酶偶联，酶活力实验结果表明其酶活回收率为15.1%。

Then MMSC were modified by APTES to introduce amino groups and alkaline protease was immobilized using a coupling agent PDC . The enzyme activity results showed that the activity recovery rate was 15.1 % .