细胞分离
- 网络Cell Separation;cell isolation;Cell Fractionation;cell segregation
-
因此,用金包覆磁性纳米微粒将在生物医药、细胞分离、DNA检测、磁记录等领域有着广泛的应用前景。
The magnetic nanocomposite with gold coating can be used to targeted drug delivery , cell separation , DNA detection and so on .
-
而磁性材料作为一种常见的功能性材料在生物医学和生物技术方面更有着广泛的应用,如蛋白质和酶的固定化、免疫测定、RNA和DNA的提纯、细胞分离及磁性靶向药物。
Among that , magnetic materials as a common functional materials is more in a wide range of applications in biomedical and biotechnology , such as enzyme immobilization , protein and immune determination , the purification of RNA and DNA , cell separation and magnetic targeted drugs [ 6-9 ] .
-
单细胞分离及单细胞PCR技术在淋巴结生发中心研究中的应用
Preliminary Study of Single Cell Isolation and Single Cell PCR in Lymph Node Germinal Centers
-
从人白血病细胞分离得到的递向转录酶在如下几方面类似于RNA肿瘤病毒逆向转录酶。
The reverse transcriptase isolated from human leukaemic cells resembles that from RNA tumour viruses in several respects .
-
蛋白细胞分离现象仅见于NMO两型患者,MS者无此现象;
The protein cell segregation is observed only in NMO but not in MS.
-
从原始生殖细胞分离昆明小鼠EG细胞及其传代培养
Isolation of Kunming Mice Embryonic Germ Cells Derived From Primordial Germ Cells and Their Passage Culture
-
肝细胞分离技术的逐渐成熟,使肝细胞移植(hepatocytetransplantation,HCT)成为可能。
With the development in isolation of hepatocyte , it became possible to put hepatocyte transplantation ( HCT ) in practice .
-
CD34免疫亲合柱在脐血造血干祖细胞分离与扩增中的应用
The application of CD34-immunoaffinity column in the isolation and expansion of hematopoietic stem and progenitor cells of cord blood
-
目的简化大鼠睾丸支持细胞分离培养方法,获取高成活率、高数量、高表达的Fas配体阳性支持细胞。
Objective To simplify the method for separation and cultivation of rat testicular Sertoli cells with high viability , quantity and expression efficiency .
-
猪原始生殖嵴(PGCs)细胞分离、建系培养
Establishment of porcine primordial germ cell ( pgcs ) in vitro culture
-
试验证实,SD大鼠心肌条件培养基在昆明白小鼠胚胎干细胞分离培养过程中效果较好。
It indicated that the effect of media conditioned of SD rat cardiomyocyte to isolation and culture of Kunming white mouse embryonic stem cell was very good .
-
作者应用PCR技术对30例疑似立克次体感染或不明热患者的血液标本和/或立克次体细胞分离培养物进行立克次体特异性DNA检测。
Blood samples and / or isolated rickettsial cell cultures from 30 patients suspected of rickettsial infection or unknown fever were studies for rickettsial specific DNA by PCR with 3 pair primers R. r. 190.70 p 、 R.
-
与非重症组患者相比,重症组的最低淋巴细胞数目更低,最大淋巴细胞降幅更大,更多出现淋巴和中性粒细胞分离现象,两组相比具有显著性差异(P0.05)。
Compared with non-critical SARS group , critical SARS group had lower minimum lymphocyte counts , larger range of lymphopenia and greater difference between neutrophilic granulocyte and lymphocyte counts ( P 0.05 ) .
-
用veroE-6细胞分离流行性出血热病毒
Isolation of Epidemic Hemorrhagic Fever Virus in Vero E-6 Cells
-
骨膜成骨细胞分离培养及其BMP-2cDNA基因转染的初步研究
Isolation and culture of periosteal osteoblasts and transfected by BMP 2cdna : a primary study
-
滑膜细胞分离后观察:滑膜细胞24h后贴壁生长,滑膜巨噬样细胞在类风湿关节组的比例较正常滑膜组高。
Observation after separation of synoviocytes : The synoviocytes grew on the wall after 24 hours , and macrophage-like synoviocytes were more in the RA group than in the normal synovium group .
-
采用乳鼠接种试验和BHK-21细胞分离培养试验,从猪脑组织病料中分离获得了具有特异致病性和致细胞病变(CPE)的毒株。
By nude mice inoculation and isolation and culture in BHK-21 , the new JEV strain of specific pathogenicity and CPE was obtained from swine brain specimens .
-
为对疫情进行病原学检测与分析,采集了临床标本,采用HEp2、RD、Vero等细胞分离病毒,并用肠道病毒通用引物进行特异性逆转录聚合酶链反应(RTPCR)扩增。
To detect and analyze the pathogen , we collected clinical samples and isolated viruses using Hep-2 , RD , and Vero cell lines , then the viruses were amplified by specific RT-PCR with enterovirus universal primer .
-
BRL-CM在昆白小鼠胚胎干细胞分离培养中的应用
Application of Buffalo Rat Liver Cell Conditioned Medium in Isolation and Culturing of KM Mouse ES Cells
-
方法:1、采用MACSCD34+细胞分离系统分离脐血,流式细胞仪技术检测分离脐血CD34+细胞的纯度。
Methods : 1 . CD34 + hematopoietic stem cell was isolated and purified from human cord blood using MACS system . The purity of isolated CD34 + cells were determined by flow cytometry . 2 .
-
方法大鼠肝星状细胞分离采用胶原酶原位灌注法,用流式细胞仪和琼脂糖凝胶电泳法检测MG132对诱导激活的HSC凋亡的影响。
Methods HSC were isolated from rats with collagenase in situ liver recirculating perfusion . Apoptosis was analyzed by flow cytometry ( FCM ) and agarose gel electroporesis .
-
2采用MACSCD34+细胞分离系统分离骨髓,流式细胞仪检测分离骨髓CD34+的纯度。分离纯化的CD34+细胞在OS培养基中诱导培养并进行成骨细胞功能鉴定。
Marrow cell isolated by Mini MACS system on the basis of CD34 antigen expression and detected the purity of CD34 + population by FACS , these CD34 + cells were cultured in OS medium and the properties of osteoblastic differentiation were detected .
-
[结论]该研究显示,我们的细胞分离技术能快速分离培养出毛囊外根鞘细胞,并且这些细胞含有bulge干细胞。
In the new dermal layer , the matrix decreased 4 weeks after transplantation . [ Conclusion ] This study gives evidence that our isolation technique allows isolating and culturing of outer root sheath cells , which contain some bulge stem cells .
-
结果发现两株从鸡胚分离到的甲3(H3N2)亚型D相毒株与2株从MDCK细胞分离到的O相毒株抗原性已明显不同;
Results A distinctive antigenic difference was found between two strains of " D " phase influenza A ( H3N2 ) virus isolated from embryonated chicken eggs and two strains of " O " phase influenza A ( H3N2 ) virus isolated by MDCK cell culture .
-
表达绿色荧光蛋白的猪胚胎生殖细胞分离
Isolation of porcine transgenic embryonic germ cells expressing green fluorescent protein
-
两种豚鼠听毛细胞分离法比较
Comparison of two methods for isolating hair cells from guinea pigs
-
哺乳动物胚胎干细胞分离与培养的研究进展
Research Advance in Isolation and Culture of Embryonic Stem Cells in Mammalian
-
人脐带间质干细胞分离纯化及基本生物学特性研究
Biological study of human umbilical cord derived mesenchymal stem cells
-
从原始生殖细胞分离和克隆兔的胚胎生殖细胞
Isolation and clone of embryonic germ cell from rabbit primordial germ cell
-
成人胰腺干细胞分离、培养及鉴定
Isolation 、 Incubation , and Identification of Pancreatic Stem Cells in Adults