荧光染料

CdS纳米颗粒对荧光染料光谱特性的影响

Influence on the Spectral Characteristics of Fluorescence Dye from CdS Nanometer Particles

本论文分为三部分，分别为介孔材料MCM-41与半导体硫化镉的组装，MCM-41与荧光染料曙红的组装以及MCM-41与有机发光配合物铕-苯甲酸-邻菲咯啉的组装。

There are three parts in the dissertation : MCM-41 mesoporous material and semiconductor assembly , MCM-41 and fluorescence dye Eosin Y assembly , and MCM-41 and rare earth complex assembly .

荧光染料离子缔合作用在荧光法测定微量DNA中的应用

Association of Dyes Bearing with Opposite Charges and Its Application in the Determination for Micro Amounts of Nucleic Acids

同时针对荧光染料罗丹明B，比较了喂食法和染色法制备荧光蚕丝纤维的结构和性能差别。

At the same time , we compare the structure and performance of the fibroin of feeding method and dyeing method .

HRM分析过程中，随着双链DNA分子的扩增，由于荧光染料结合到重新产生的双链DNA分子，荧光信号不断的增强。

In the process of HRM analysis , fluorescent dyes bind to double-stranded DNA with the DNA amplification .

利用激光共聚焦扫描显微镜结合各种荧光染料探针和荧光共振能量转移（FluorescenceResonanceEnergyTransfer，FRET）技术研究了激光照射生物效应的分子机制。

The mechanism of the effect was investigated using specific fluorescent probes staining and fluorescence resonance energy transfer ( FRET ) techniques .

SAs用微电极细胞内注射的方法注入荧光染料LuciferYellow。

SA cells were intracellular injected with a sharp electrode containing Lucifer Yellow .

实时定量PCR是利用能特异标记PCR产物的荧光染料来动态显示PCR产物的累积，从而得到PCR扩增曲线的技术。

Real-time PCR technique can produce and display the kinetic accumulation of PCR amplicon which is specifically labeled by the fluorescent dye-called amplification curve .

用荧光染料（Hoechst33342）和激光处理精子对牛卵母细胞体外受精的影响

Effects of Hoechst 33342 and laser on bovine in vitro fertilization

本论文以激发态的鲁米诺为能量供体，荧光染料为能量受体，建立了几种新型的电化学发光能量转移型DNA检测方法。

In this thesis , the ECL energy transfer method for sensing DNA was developed , in which excited luminol acted as energy donor and fluorescent dyes acted as hybridization indictor and energy acceptor .

杰弗里向瓶中加入了能与DNA相结合的荧光染料，然后将小瓶放进一种叫做流式细胞仪的四四方方的装置中。

After adding a fluorescent dye that attaches to DNA , Jeffrey loaded the vial into a boxy device called a flow cytometer , which sprayed the onion juice and blood through a laser beam .

在建立单细胞凝胶电泳的方法时，对DNA彗星图像进行了共聚焦显微镜观察和三维重建，比较了几种不同荧光染料的染色效果。

Laser scanning confocal microscope ( LSCM ) was introduced to investigate the 3-D distribution of DNA within the comet and four fluorescent dyes were compared when the single cell gel eletrophoresis was set up .

比较了几种荧光染料标记白色念珠菌和鼠伤寒沙门氏菌的效果及其对病菌生长和与Hela细胞粘附能力的影响，旨在为建立简便有效的荧光标记方法奠定基础。

Fluorescence labelling methods of C. albicans and S.typhimurium and its effect on adherence to Hela cells were investigated in the present study .

用猪AMI模型研究荧光染料CFSE和Brdu作为移植细胞标记物的实用性

The study on utilities of CFSE and Brdu as cellular labels in vivo in porcine myocardial infarction model

用荧光染料fura-2法测定心室肌细胞内游离钙离子浓度

Measurement of the intracellular free calcium concentration of ventricular myocytes with fluorescent dye fura-2

荧光染料CFSE检测淋巴细胞增殖的研究

Determination of Lymphocyte Proliferation by Fluorescein Based Dye CFSE

利用1.5%的琼脂糖凝胶电泳观察DNA梯形条带，并应用DNA荧光染料Hoechst33342对正常肝细胞进行染色，以检测TGFβ1诱导的凋亡。

Normal hepatocytes treated with TGF - β 1 ( 5 ng / ml ) were stained with fluorescent DNA-binding dyes Hoechst 33342 . The apoptosis of hepatocytes was determined by electrophoresis on 1.5 % agarose gel to observe DNA ladder .

以R-藻红蛋白（R-PE）标记小鼠抗人CD4单克隆抗体，形成单色或和用其他荧光染料标记的CD系列单抗组成双色、多色的荧光试剂，应用于流式细胞仪检测分析。

The conjugate of R-phycoerythrin ( R-PE ) and mouse anti human CD4 monoclonal antibody was prepared as a reagent in detection procedures by flow cytometry in this study .

荧光染料CFSE标记大鼠间充质干细胞的体外研究

In vitro tracing of rat bone marrow mesenchymal stem cells using the fluorescent dye CFSE

荧光染料DAPI的标记效率可达93.4%，细胞传代8次后荧光亮度仍无明显衰减。

Labeling rate of DAPI was 93.4 % and no attenuation of fluorescence brightness as observed after 8 generations in culture .

使用羰花青荧光染料DiⅠ逆向神经标记技术研究了这些投射向GLv核的视顶盖Ⅰ细胞的形态特征。

To reveal the morphological features of ' I cells ' which project to GLv , they were retrogradely labeled with cyanine perchlorate DiI in chicks .

第二，采用酸性荧光染料对改性织物进行染色，研究不同染色pH、时间、温度等条件对染色织物的K/S值、R值、色牢度的影响，确定最佳染色工艺条件。

Second , the optimum dyeing process with acid fluorescent dyes is determined by studying impact of different dyeing pH value , time , temperature on the K / S value of dyed fabric , the influence of the R value , color fastness .

用荧光染料AnnexinV-PE和7-AAD借助于流式细胞仪观察Curcumin对C6细胞早期凋亡的影响；

Annexin V-PE and 7-AAD were used to measure the apoptotic cell at the early phase induced by Curcumin with Flowcytometry ;

结论荧光染料CFSE是一种可以监测淋巴细胞及其亚群增殖状况的很好的示踪剂。

Conclusion CFSE is an excellent stain to be used to analysis the lymphocytes and their subsets proliferation .

本文建立了一种以阳离子型荧光染料藏红T（ST）作为荧光试剂的荧光猝灭新方法，ST首次被用于PNA-DNA杂交和解链的检测。

A new fluorescence quenching method was developed based on safranine T , a cation fluorescence dye , and used to study hybridization and dissociation of PNA-DNA for the first time .

方法：用荧光染料Fluo-3-AM标记细胞，在粘附式细胞仪上测定细胞[Ca2+]i的变化。结果：给予PD后心肌[Ca2+]i升高。

METHODS : The cells were labelled with fluo-3-AM , and [ Ca 2 + ] I was determined by use of confocal microscopy ( ACAS 570 ) .

本文将其中一条核酸适体通过生物素和亲和素的相互作用固定在玻片表面，另一条标记有量子点或荧光染料TMR。

In this paper , an aptamer was fixed on slide with the interaction of biotin and avidin , and another aptamer was labeled with quantum dots or TMR .

本实验研究了线粒体特异荧光染料Rhodamine123（Rh-123）对HeLa细胞的选择性毒性作用。

The selective toxicity of the mitochondria-specific cationic fluorescent dye rhodamine 123 ( Rh-123 ) on HeLa cells in culture was studied .

方法：以CM-DiI荧光染料示踪BMSC的体内分布情况，并辅以PCR检测Y染色体的方法进一步鉴定；

Methods : In this work , used a available fluorescence dye , CM-DiI , as a tracing label , and Y-chromosome-specific PCR to detect the distribution of BMSC in vivo .

使用荧光染料作为增强介质，当SRS产生的Stokes辐射通过处于粒子数反转状态的荧光介质时，可以实现Stokes波的有效放大。

So when the Stokes waves serving as signal light traverse fluorescence medium with an inverted population , the Stokes waves will be increased just like the amplification of a laser beam in a laser gain medium .