菌落杂交

应用生物素标记的DNA探针通过菌落杂交试验检测沙门氏菌

Detection of Salmonellae by Colony Hybridization Assay with Biotin-labelled DNA Probes

本实验应用Bio-11-dUTP标记的两个沙门氏菌属特异性核酸探针（Bio-8-0kb探针和Bio-3.4kb探针）成功地通过菌落杂交试验检测了60个血清型中的100株沙门氏菌。

By using two Bio-11-dUTP labelled DNA probes specific for the Salmonella genus , i. e. , Bio-8.0 kb probe and Bio-3.4 kb probe , 100 Salmonella isolates including 60 Salmonella serotypes were detected successfully by colony hybridization assays .

方法使用菌落原位杂交、DNA打点杂交和PCR扩增等方法。

Methods Colony hybridization 、 DNA dot hybridization and polymerase chain reaction ( PCR ) were used .

以线粒体基因为探针对文库进行菌落原位杂交验证，均筛选到了阳性克隆。

With mitochondrial genes as probes , positive clones were found .

乙型肝炎病毒克隆的菌落原位杂交

Colony hybridization in situ for screening for clones containing HBV DNA

其中有38株菌的试验结果和菌落原位杂交结果一致，符合率为95%。

38 of 40 strains were shown to be identical .

这篇文章包含菌落转移或菌落杂交，Southern杂交和Northern杂交以及生物芯片。

It contains colony lifts or hybridization , Southern and Northern hybridizations and microarrays .