超薄切片

为阐明水痘-带状疱疹病毒济南分离株（VZVJ1）在兔脑神经细胞（RNC）中的形态与形态发生特征，我们利用超薄切片电子显微镜技术对感染VZVJ1的RNC进行了观察研究。

To Investigate the morphology and morphogenesis of the isolated varicella zoster virus ( VZV ) in rabbit brain nerve cell ( RNC ), ultrathin section electron microscopy was used for study . A small number of nucleocapsids were observed in the nuclei of RNC at 6 h post infection .

切片（半薄切片和修块和超薄切片）。

Sectioned ( semithin section and repair the block and ultrathin section );

在超薄切片中颗粒则有两种不同的结构：一种是外膜泡（Outermembranevesicle）；

In ultrathin sections granules possess two different kinds of structures : One is a Outer-membrane vesicle : another is a Dense body .

本实验结果表明，AFM技术如与超薄切片技术相结合，可对样品的内部结构进行研究，而不仅仅是一种表面成像工具。

Combined with the technique of ultra-thin sections , AFM is capable of studying the inner structure of sample .

方法：应用超薄切片和透射电镜技术，比较N株和RC株虫体食物泡和疟色素形态及形成的差异。

Methods : The RC strain and the N strain were compared concerning the formation and morphologic features of digestive vacuoles and haemozoin with transmission electron microscope .

方法石蜡包埋标本超薄切片，ABC法检测中耳癌S-100蛋白和增殖细胞核抗原PCNA的表达情况。

Methods Expression of S-100 protein and PCNA were detected by ABC method in ultrathin section samples of middle ear cancer tissue .

作者使用超薄切片，冷冻断裂和剥离技术在分析电镜中以透射（TEM）、扫描透射（STEM）和扫描（SEM）成象模式研究了兔毛纤维的微观结构。

Using ultramicrosection , cryofracture and peeling technique , the fine structure of rabbit hair was investigated in an analytical electron microscope by TEM , STEM and SEM imaging models respectively .

本实验用超薄切片和冷冻复型电镜技术，研究了豚鼠海马CAI区锥体细胞间的缝隙连接（GJ）。

This article presents a brief introduction of the gap junctions on CA_1 pyramidal cells of guinea pig hippocampus observed by freeze replica technique .

采用组织超薄切片技术，电镜下观察研究昆虫棉铃虫HelicoverpaarmigeraHǖbner脂肪体细胞的超微结构，结果表明。

The ultrastructure of fat body tissue of Helicoverpa armigera H ü bner was observed under the electron microscope by means of ultra-thin tissue slice technology .

目的：对BalbC小鼠胚胎干（ES）细胞的超薄切片进行AFM成像，以获得ES细胞内部结构的信息。

Objective : To image the ultra-thin sections of Balb / c murine ES cell by means of atomic force microscopy in order to obtain the information of the inner structure of ES cells .

利用AFM得到了小鼠胚胎干细胞超薄切片的高分辨率图像，探索用AFM研究细胞内部结构，拓展其应用领域；

By means of AFM , the ultra-thin sections of murine ES cells were investigated in order to make AFM capable of gaining the information of the inner structure of cells .

于创伤后1、6、24、72h取大鼠右眼视神经，用硝酸镧灌注固定法制作电子显微镜样品，超薄切片，透射电子显微镜观察视神经超微结构的改变。

The ultrastructural changes of the optic nerves were observed 1 , 6 , 24 , and 72 hours after the injury by electron microscopy .

本文用超薄切片电镜技术对水痘一带状疱疹病毒（VZV）分离株J1的核衣壳进行了形态学研究。

The nucleocapsids of varicella-zoster virus ( VZV ) strain J1 isolated hy Dr. Hun Shi-jie et al in Jinan in 1990 were studied with electron microscope .

电镜下观察接毒鸡胚的超薄切片可看到AEV病毒颗粒，大小在25-30nm。

The virus was observed under transmission electrical microscope which diameter was 25 - 30 nm .

取海马CA1区辐射层作超薄切片，电镜观察，并定量研究单位切片面积内的突触数。

Ultrathin sections were obtained from the stratum radiatum of CA1 area in the hippocampus and were observed under electron microscope .

该病毒感染细胞超薄切片电镜下见疱疹病毒样颗粒，感染细胞涂片与抗HHV－6单克隆抗体产生明显荧光，证实为HHV－6。

Examination of ultrathin sections of the virus infected cells by electron microscope showed herpes like virus particles .

普通病理技术包括石蜡切片、HE、TB、LFB染色、半薄和超薄切片、铅铀染色；小胶质细胞用凝集素组化显示；

Ordinary neuropathological method included procedures of paraffin section , ultrathin and semithin section , HE , TB , and LFB staining , uranyl and lead staining .

通过超薄切片和透射电子显微镜技术对鸭病毒性肠炎病毒（DEV）CH强毒株在鸭胚成纤维细胞（DEF）中的形态结构进行了研究。

Ultrathin sectioning and transmission electron microscopy of infected duck embryo fibroblasts ( DEF ) were employed to investigate the morphology of virulent duck enteritis virus ( DEV ) strain CH.

为研究鸭病毒性肠炎病毒（DEV）CH强毒株在感染鸭体内的分布和形态学发生规律，应用透射电镜和超薄切片技术对人工感染DEV的成年鸭各组织器官进行观察。

In order to study the morphogenesis and distribution of duck enteritis virus ( DEV ), tissues of ducks infected experimentally with DEV virulent strain were collected and observed by electron microscopy .

与0.128mg/mlRES作用后透射电镜超薄切片照片显示菌体细胞壁层次不清，细胞器结构模糊，细胞质内含物大多消失，出现大量空泡。

After affect by 0.128 mg / ml RES the layer of cellwall is indistinct , the structure of cell organ is blurred , most cytoplasmic inclusion disappeared , generous vacuole emerged .

应用超薄切片技术对水泡性口炎病毒（VSV）在BHK-21细胞上的形态发生和早期发育进行了观察。

The morphopoiesis and progenesis of the vesicular stomatitis virus in baby hamster kidney cell-21 ( BHK-21 ) were observed by using electron microscope .

为进一步研究的生物学特性，应用鸡胚成纤维细胞（CEF）和鸡胚肾细胞（CEK）培养禽腺病毒江苏分离株，进行培养特性、血凝特性、理化特性、超薄切片观察和PCR检测。

Chicken embryo fibroblast ( CEF ) cell and chicken embryo kidney ( CEK ) cell were used to study the biology characteristic of avian adenovirus isolate of Jiangsu .

方法选取临床培养的经PCR鉴定、筛选的fimA基因Ⅰ、Ⅱ和Ⅳ型P.gingivalis野生菌株，制备超薄切片，在透射电镜下进行菌体表面结构观察。

Methods Different fimA genotype P.gingivalis strains cultured from subgingival plaque samples and detected by fimA type-specific PCR were selected , then the cells were observed under transmission electron microscope by ultrathin section .

电子显微镜观察发现，在病株嫩枝和叶柄韧皮部筛管细胞超薄切片中，存在着大量的类菌原体（MLO）。

A lot of mycoplasma-like organisms ( MLO ) were observed within the ultrathin sections of sieve cells in the infected tender shoots or phloem tissues with electron microscopy .

作者应用两种胶体金探针，于胃癌组织冰冻超薄切片上，对胃癌单抗MG7相应抗原与DBA受体进行了电镜双标记定位。

The double labeling localization of the corresponding antigen of MG7 and DBA receptors was studied on ultrathin frozen sections of gastric cancer tissue by two kinds of colloidal gold probes .

采用非树脂包进埋去包埋超薄切片电镜技术，并结合选择性生化抽提方法以及间接免疫荧光染色方法显示，在四膜虫（Tetrahymenathermophila）细胞内存在典型的核纤层结构。

The nuclear lamina in the nuclei of Tetrahymena thermophila was investigated using the techniques of selective extraction and embedment-free electron microscopy , as well as immunofluorescence staining .

应用超薄切片观察了人胚19、21、26、和34周小脑皮质Purkinje细胞层突触的发育。

The development of synapse of Purkinje cell layer of cerebellar cortex in Chinese Human Fetus at fetal age of the week 19 、 21 、 26 and 34 are observed by ultrathin section technique .

本文应用分离染色体骨架的TEM技术、超薄切片甩镜细胞化学、RNase一胶体金标记和银染电镜技术结合研究了牛肾（BK）细胞的分离染色体骨架和完整的中期染色体骨架。

The isolated or intact metaphase chromosome scaffold of BK ( Bovine Kidney ) cell has been studied by using several techniques , such as isolated chromosome scaffold TEM , ultra thin-sectioning cytochemical EM , RNase-gold complexes labeling and Ag-staining .

此外，通过超薄切片、分区域扫描和计算机软件分析等技术，本研究初步解决了AFM在细胞生物学应用中的两大难题&样品内部结构的不可获得性和样品扫描范围的限制性。

Furthermore , with the help of ultra thin section technology , zoning scan and computer software analysis , this study has preliminarily solved two difficult problems during applied AFM in cell biological researches & the unobtaining of inner structure and the restriction of scanning range .

本实验将胎龄为15d的胎鼠大脑皮质移植到中年Wistar大鼠大脑皮质3,1,2区内。移植物成活120d时取材，超薄切片，电镜观察。

Whole layer of E_ ( 15 ) fetal rat cerebral cortex was transplanted into cerebral cortex areas 3 , 1 , 2 of middle aged Wistar rat . Grafts of 120 days were made into ultrathin slides and observed with electron microscope .