转录物

方法：大鼠Caspase-3基因的PCR片段克隆于T载体T7启动子下游，32P标记的体外转录物作为靶RNA。

Methods : Rat caspase-3 gene fragment was cloned into T-vector under the control of T7 promoter . 32P-labeled caspase-3 transcript was target-RNA .

结论：构建的我国D243株基因组全长cDNA的体外RNA转录物对传代蚊细胞具有感染性，表明可产生完整的病毒颗粒。

Conclusions : The in vitro RNA transcript prepared from the genomic full length cDNA of D2 43 virus has specific and stable infectivity , demonstrating that the intact dengue virus can be produced .

锤头状核酶RCP对HBV的P基因体外转录物的作用

Effect of hammerhead ribozyme ( RCP ) on HBV P gene in vitro

含CD（44）V6外显子的变异体转录物的异常表达与肿瘤的恶性表型和转移特性密切相关从而影响其预后；

The abnormal expression of transcript variant of CD44V6exon is intimately relevant to malignant phenotypes and metastasis of cancer , which thereafter influence its prognosis .

其作用既可从DNA水平上调节相关基因的表达和mRNA水平上调节转录物的稳定性，也可从蛋白质水平上通过磷酸化来调节酶及相关蛋白质的活性。

The regulation can be made at DNA level by regulating the related gene expression , mRNA level by regulating the transcription and protein level by regulating the activity of enzymes or related protein through phosphorylation .

将以上两种RNA转录物混合并经37℃保温后，检测结果表明所得核酶RNA对PLRV-Ch复制酶基因负链RNA在体外具有较强地特异切割活性。

After mixture of the RNA transcripts and incubation at 37 ℃, results showed that the ribozyme has highly catalytic activity to the PLRV-Ch negative RNA in vitro .

应用RTPCR方法从人外周血单个核细胞扩增底物caspase3mRNA目的片段，体外转录物作为靶RNA，核酶与靶RNA按1∶1比例进行体外切割实验。

The substrate caspase 3 mRNA fragment was generated from human peripheral blood mononuclear cells by RT PCR . After transcription of ribozymes and substrate by 32 P , cleavage reaction in vitro was detected .

作为新近建立的研究基因表达的有效工具，基因表达系列分析（SAGE）技术能同时对大量的转录物进行定性和定量分析。

As an efficient tool that has been developed recently , serial analysis of gene expression ( SAGE ) allows the qualitative and quantitative analysis of a large number of transcripts .

用脂质体转染法将转录物导入RK-13细胞，24h后可观察到典型的RHDV致细胞病变效应。

RK-13 cell was transfected with transcribed product in vitro using lipofectamine reagent , typical RHDV CPE would be seen after 24 hour .

基因表达系列技术（SAGE）是一种以测序为基础，采用数字化分析手段，在转录物水平上研究细胞或组织基因表达模式的有效工具。

Serial analysis of gene expression ( SAGE ) is a powerful sequence-based tool , which allows the analysis of gene expression patterns in cell or tissue at the level of transcripts with digital analysis .

对来自RSV感染消减文库的一个免疫相关基因Mucin基因进行Northern杂交分析，结果显示只能在RSV感染的灰飞虱中检测到它的转录物，而用细菌感染的灰飞虱中检测不到。

Northern Blot was performed to determine the expression pattern of mucin gene that was obtained from RSV-infected subtracted library . The result showed that the transcript of mucin could only be detected in RSV-infected planthopper .

应用GDPs转录物标记法进行水稻白叶枯病菌早期侵染表达谱分析

DNA Microarray Expression Analysis of Xanthomonas oryzae pv . oryzae in Rice Leaves at Early Infection Stages Using Selective Bacterial Transcript Labeling with Genome-Directed Primers

目的：研究我国登革2型病毒43株（D243）基因组全长cDNA体外RNA转录物的感染性，为进一步阐明登革2型病毒的致病机制及探索其新型疫苗奠定基础。

Objective : To study the infectivity of the in vitro RNA transcript of the genomic full length cDNA of strain 43 of Chinese dengue 2 virus ( D2 43 ), and hence to provide the basis for elucidating the molecular pathogenesis of dengue virus and developing novel vaccines .

方法小鼠Caspase-12基因的逆转录聚合酶链反应（RT-PCR）扩增片段克隆于PGEM-T载体的T7启动子下游，通过α-32PUTP标记的体外转录物作为靶RNA。

Methods The mouse caspase-12 gene fragment was obtained by RT-PCR and cloned into the PGEM-T vector under the control of T7 RNA polymerase promoter . The transcription product of the target was labeled with α - 32P UTP , while ribozymes were not labeled .

中期因子转录物及其蛋白在肝细胞癌中的定位与表达研究

Expression and localization of midkine in hepatocellular carcinoma

在本研究中，我们分别将叙利亚仓鼠急性感染和持续感染细胞的基因组转录物跟小鼠全基因组表达谱芯片进行杂交。

In this research , we hybridized RNA from syrian hamsters to whole mouse genome array .

小麦黄花叶病毒体外转录物对小麦愈伤细胞的侵染

Infectious Wheat yellow mosaic virus RNA Transcripts in vitro from Full-length Genomic cDNA Clones in Inoculated Wheat Cell Calli

专一切割苹果锈果类病毒多体自切割核酶的克隆和转录物的体外活性测定

Design and Preparation of the Multimeric Self-cleavable Hammerhead Ribozyme Targeting Apple Scar Skid Viroid and Its Activity Detection in vitro

其中一个转录物包含第3、4号外显子，第二个包含第1、3、4号外显子，第三个转录物包含有全部4个外显子。

One transcript contains exon 3 and 4 , the second contains exons 1 , 3 and 4 and the third contains all four exons .

结构基因组学、蛋白质组学和转录物组学的研究产生了大量关于基因表达和蛋白质表达的数据，但同时，它们与功能基因组学的研究相比也显示出了局限性。

Although structural genomics , proteomics and transcriptomics yield vast amounts of date about the expression of genes and proteins , they are still under pressure to infer gene function .

后来的X射线结果显示，具有添加位点的转录复合物能够纳入相匹配的NTP。

Later X-ray structures revealed the transcribing complex with the addition site available for entry of a matched NTP .

文章综述了依赖于DNA的RNA聚合酶Ⅲ的启动子和转录复合物，并介绍了RNA聚合酶Ⅲ的转录在基因表达和基因治疗（核酶、反义核酸、RNA干扰技术等）研究中的应用价值。

In this article , we review the promoters and transcription complex which are dependent on RNA polymerase ⅲ . The application of RNA polymerase ⅲ - catalyzed transcription in gene therapy and gene expression by the ribozyme , antisense oligonucleotides and RNA interference is also discussed .

垂体转录活化物-1基因在人垂体腺瘤中的表达

Expression of the pituitary transcription activator-1 gene in human pituitary adenomas

一种新的核蛋白CTCF-多功能转录调节物

A Novel Nuclear Protein CTCF-versatile Transcription Regulator

完整病毒内部为致密而有序排列的双链RNA，而空病毒内部却几乎没有电子密度，只在突起的底部具有12个向内伸展的电子密度，作者认为该电子密度属于CPV转录酶复合物结构。

The dense and ordered genomic dsRNA is located inside the full CPV . The internal space of the empty CPV has almost no electron density except for 12 electron densities attributed the transcriptional enzyme complexes extending inward from the base part of CPV spikes .

基因抑制从形式上可以分成主动抑制和被动抑制，近程抑制和远程抑制等，但是其分子基础是抑制转录机器复合物（transcriptionalmachinery）或形成抑制性在染色体结构如异染色质等。

There are repression mechanisms like passive and active repressions . short-distance or long-distance repressions , the molecular basis of which is either inhibition of the activity of general transcriptional machinery , or formation of repressive chromatin structures .

配基化的雄激素受体转录引发复合物的组成决定了基因调控的特异性，设计用于引发组织和启动子特异基因转录的合成配基提供了开发更有效的雄激素治疗的希望。

Since the composition of the transcriptional initiation complex recruited by liganded AR determines the specificity of gene regulation , synthetic ligands aimed at initiating transcription of tissue and promoter specific genes offers hope for developing better androgen therapy .

最近有实验指出，肌动蛋白能被募集到基因的启动子区，是转录前起始复合物的成分。

Recently , it was reported that actin could be recruited to the promoter region of the gene , and is a component of pre-initiation complexes ( PICs ) .

RNA聚合酶对转录调节作出抉择依赖于对转录延伸复合物内在的或外来的信息输入，其功能在很大程度上都像是一个信息加工者。

The decision of RNA polymerase for transcriptional regulation depends on the intrinsic or extrinsic imputs of information to transcription elongation complex . Thus , the function of RNA polymerase looks like an information processor to a great extent .

近期实验报道，在基因的转录过程中，细胞核肌动蛋白分别作为染色质改构复合物，转录前起始复合物和信使核糖核蛋白的组分参与了3种真核生物RNA聚合酶的转录调控。

Recent reports have shown that in gene transcription , nuclear actin plays a key role as a component of chromatin remodeling complexes , transcription pre-initiation complex and messenger RNP ( mRNP ) particles associated with all three eukaryotic RNA polymerases ( pol ) .