EcoRI

Molecular Cloning of EcoRI + BamHI Fragments of Egg Drop Syndrome Virus DNA

鸡产蛋下降综合征病毒NE4株DNA的EcoRI+BamHI部分酶切片段的克隆

【 Method 】 Genomic DNA of 48 tobacco materials were amplified by 4 AFLP selective primers ( EcoRI + 3 / MseI + 3 ) .

【方法】选用4对AFLP选择性扩增引物（EcoRI+3/MseI+3）对48份烟草材料的基因组DNA进行选择性扩增。

The restriction fragment length polymorphism ( XbaI , EcoRI ) of apolipoproteinB gene in relation to the atherothrombotic cerebrol infarction

动脉硬化性血栓性脑梗塞与载脂蛋白B基因XbaI、EcoRI多态的相关性研究

According to the ORF , designed site ( KpnI and EcoRI ) primers , the constructed recombinant expression plasmids were induced by the chemical inducer IPTG . The expression products were analyzed by SDS-PAGE .

根据开放阅读框设计带酶切位点（KpnI和EcoRI）的引物，构建重组表达质粒，经IPTG诱导表达，利用SDS-PAGE分析表达产物。

After ligating the cDNA into the adaptor of EcoRI / NotI and then into λ gt11 DNA arms of the expressive vector , the ligated products were packaged in vitro and used to infect the E.

将cDNA与EcoRI／NotI接头连接，再与表达载体λgt11DNA臂连接，经体外包装，感染大肠杆菌Y1090。

We have obtained 662 markers using 19 PstI / Tru1I ( Mse1 ) primer pairs and 4 EcoRI / Tru1I primer pairs , of which 43.2 % has 3:1 in segregation , 14.5 % appears to be non-parental heteroduplex , and 48.8 % is distorted .

选择扩增使用了19对PstI/Tru1I（Mse1）引物和4对EcoRI/Tru1I引物。共得到标记662个，其中3：1标记占43.2%，异倍标记14.5%，偏分离标记（P<0.05）占48.8%。