maldi

MALDI imaging mass spectrometry on biological tissue sections : the new progress

生物组织的基质辅助激光解吸电离质谱成像新进展

The Determination of Recombinant Human Endostatin Protein Molecular Mass by Using MALDI - TOF - MS

MALDI-TOF-MS法对重组人内皮抑素蛋白分子质量测定

The Application of MALDI - TOFMS in Snake Venom Analysis

基质辅助激光解吸电离飞行时间质谱在蛇毒组分分析中的应用

C-terminal Sequencing of Protein and Peptide by MALDI TOF Mass Spectrometry

利用飞行时间质谱进行蛋白质和多肽C端序列测定

Determination of Molecular Weight of Sodium Poly ( acrylic acid ) by MALDI / TOF Mass Spectrometry

MALDI-TOF-MS法测定聚丙烯酸钠的分子量

The molecular weight of synthesized peptides was identified to be correct by MALDI - TOF mass analysis .

合成的多肽经过MALDI-TOFmass鉴定表明正确无误。

It can lead to high-quality MALDI mass spectra as strong analyte signals and weak or negligible matrix background peaks .

基质抑制效应可以产生高质量的质谱，即：质谱中分析物信号较强，基质信号很弱或者消失。

Identification of IPG IEF Separated Proteins by MALDI - TOF - TOF

MALDI-TOF-TOF鉴定IPGIEF分离的蛋白质

MALDI / TOF MS can be used as a quick accurate analytical method for the determination of the purities of protein drugs .

TOFMS提供了一种测定蛋白质药物纯度快速准确的新方法。

Matrix assisted laser desorption ionization ( MALDI ) technology is one of the best mass spectrometry ionization technologies for macro molecules which was developed almost two decades ago .

基质辅助激光解吸电离（MALDI）技术是近年来发展起来的新的质谱离子化技术。

The ionization mechanisms of ESI and MALDI are fundamentally different , which makes the information of the same sample obtained by the two approaches complementary and analogous .

MALDI与ESI离子化机理显著不同，两者对同一样品进行分析时，获得的信息既可相互确认又能形成补充。

Etioplasts have the capacity to synthesize plastome-coded proteins . Recent Developments in Profiling and Imaging of Molecules from Tissue Sections by MALDI Mass Spectrometry

黄化质体也有能力合成质体基因组所编码的蛋白质。基质辅助激光解吸电离质谱用于生物组织的质谱成像应用进展

Cytochrome C absorbed on nitrocellulose ( NC ) membrane was directly analyzed by matrix assisted laser desorption ionization time of flight mass spectrometry ( MALDI TOF MS ) .

直接将细胞色素C吸附在硝酸纤维素（NC）膜上，进行了基质辅助激光解吸电离－飞行时间－质谱（MALDI－TOF－MS）分析。

The carboxypeptidase digestions of peptides were analysed for C terminal sequences by matrix assisted laser desorption / ionization time of flight ( MALDI TOF ) mass spectrometry .

利用基质辅助激光解吸飞行时间（MALDITOF）质谱技术，测定羧肽酶Y消化蛋白质和多肽。

Methods : The co immunoprecipitation assay was employed to isolate TRF1 protein complex and the immunoprecipitate was subjected to MALDI TOF mass spectrometry for protein identification .

方法：以TRF1抗体应用免疫共沉淀方法，从细胞蛋白抽提物中分离TRF1蛋白复合物，并作蛋白质肽指纹谱鉴定；

Using simulated micro mixed endopeptidase system prepared with crushed sea slug eggs , the hydrolysis and enzymolysis of insulin under different pH and reductive conditions were studied by MALDI TOF mass spectrometry .

利用海牛卵制备的模拟微量混合内切酶体系，用基质辅助激光解吸电离-飞行时间（MALDI-TOF）质谱技术研究了胰岛素在不同酸碱度和还原条件下的水解和酶解过程。

Subsequently , we investigated the capacity of this material as a MALDI matrix , and some common small molecule metabolites were analyzed by MALDI-MS , including amino acids , carbohydrates and fatty acids .

随后，我们考察了该材料作为MALDI基质的能力，分析了一些常见的小分子代谢产物，包括氨基酸、糖和脂肪酸。

The molecular weight , the rate of glycosylation and the glycosylation site of Ribonuclease B were determined by MALDI TOF MS combined with protease and endo glycosidase digestions .

本文应用基质辅助激光解吸附电离飞行时间质谱（MALDI-TOF-MS）测定了糖蛋白核糖核酸酶B的平均分子量及其糖基化程度。

Research of Orthogonal-injection and Delayed-extraction MALDI

延时提取正交发射MALDI技术研究

After in_gel protein digestion , the different_expressed spots were detected by matrix_assisted laser desorption ionization_time of flight mass spectrometry ( MALDI TOF MS ) .

用双向电泳分离两种细胞蛋白质混合物并进行比较，找出差异点，这些点经过胶内酶切后进行MALDITOF质谱鉴定。

We choose the block copolymer PSF-b-PEO with microscopic phase separation structure as the MALDI target coating materials , and then made use of the polymer film for on-plate desalting and enriching of trace peptides .

我们选择微观相分离结构的嵌段共聚物PSF-b-PEO作为MALDI靶涂层，然后利用制作的聚合物涂层进行痕量多肽的靶上除盐和富集工作。

The inhibitor , named WAI 1 , has a molecular weight of 986 5 determined by MALDI TOF mass spectrometry . It is the smallest proteinaceous inhibitor of α amylase found so far .

该抑制剂被命名为WAI1.MALDITOF质谱测得其分子量为9865，是目前报道的α淀粉酶的蛋白质类抑制剂中分子量最小的。

Differential expression protein was selected with matrix assisted laser desorption / ionization time of flight mass spectrometry ( MALDI / TOF MS ) so as to measure peptide mass fingerprinting . Swiss-Prot protein database was retrieved to identify differential expression protein .

选取差异表达蛋白质点用基质辅助激光解吸飞行时间质谱测定肽质量指纹谱，检索Swiss-Prot蛋白质数据库鉴定差异蛋白。

By using MAI , DI-MS with 2-Nitrophenyl octyl ether ( NPOE ) as liquid matrix and fibrous paper as substrate , four taxane compounds were measured . The fragmentation of their MALDI mass spectra was discussed .

本文以邻&硝基苯辛醚（NPOE）为液相基体，纤维纸为基底，用基体辅助激光解吸／电离飞行时间质谱法（MALDI－TOF－MS）测定紫杉烷类化合物，研究其激光裂解规律。

The purified IgY was digested with pepsin and the smaller antibody fragment Fab ' was obtained . The purified Fab ' was characterized by SDS-PAGE and MALDI MS methods , and its purity was over 99 % .

纯的IgY经胃蛋白酶分解得到的抗体片断（Fab），经SDS-PAGE和MALDI质谱法测定，其纯度达到99%以上。

It was identified as Rho GDI β protein after the tandem mass spectrum and after the sequence of its tryptic peptides were obtained by the ESI MS / MS techniques . It was not revealed by peptide mass fingerprint using MALDI TOF MS.

该斑点经MALDITOFMS肽质量指纹谱分析未获结果，但通过上述2种串联质谱技术获得其胰蛋白酶水解肽段的串联质谱图和肽段的全长序列，经检索均确认为RhoGDIβ蛋白。

Result : The graphs of SDS PAGE and MALDI TOF MS of velvet antler polypeptides ( VAPPs ) from Chinese and New zealand red deer were very similar , but there were obvious difference in respect of graph between sika deer and red deer .

结果：梅花鹿茸和马鹿茸多肽组分的电泳图谱和质谱有明显差异，而中国的东北马鹿茸和新西兰马鹿茸多肽的图谱十分相近；