matrigel

While Matrigel does resemble a complex extracellular environment , it also contains growth factors and unknown proteins that limit its desirability for experiments requiring precise conditions .

而基质胶确实决定复杂的细胞外环境，也包含生长因子和未知蛋白，能够抑制细胞对试验所需要的精密条件。

But the only choices hae been glass labware and a product called Matrigel , a gelatinous protein mixture secreted by mouse tumor cells .

但是，玻璃材质的试验器皿是唯一的选择，另外就是基质胶，是一种由大鼠肿瘤细胞产生的胶状蛋白混合分泌物。

Meanwhile , genistein also reduced the adhesion of MDA-MB-435 cells to Matrigel .

此外，染料木黄酮可明显降低MDA-MB-435细胞对人工重组基膜的黏附能力，其效应呈剂量依赖性。

Endothelial cells on Matrigel basement membrane matrix gel migrated into the upper part of the gel and formed tubes .

在Matrigel基膜基质凝胶上培养的内皮细胞向凝胶上层迁移，形成管状结构。

Effects of apigenin on ability of tumors cell penetrating artificial basic membrane – Matrigel and inducing angiogenesis were recorded ;

观察Apigenin对细胞穿透人工基底膜Matrigel能力及诱导内皮细胞生成血管能力的影响；

Result : Different concentration of RGDS peptide showed inhibitory effects on the adhesion of Tb cells to Matrigel and Fibronectin .

结果：不同浓度的RGDS抑制了Tb细胞对Matrigel及Fibronectin的粘附；

Results The results showed that the ameloblastoma cells and ameloblasts growed clusters in the3-D matrigel .

结果三维培养的成釉细胞和成釉细胞瘤细胞均获得成功。

Invasion was the ability of tumor cells to penetrate 12 microns pore polycarbonate membranes coated with Matrigel .

侵袭能力为肿瘤细胞穿透被覆Matrigel胶的聚碳酸脂膜（膜孔直径12μm）之细胞数。

Matrigel plug assay and chorioallantoic membrane assay were used to evaluate the effect of BDNF on angiogenesis in vivo .

体内实验采用鸡胚尿囊膜血管生成实验和小鼠Matrigelplug方法。

Methods : MTT assay was applied to study the effects of different concentration of NT-3 on adhesive capability of ACC-M cells to Matrigel .

方法：应用MTT比色法测定不同浓度NT-3对ACC-M细胞在重构基底膜Matrigel上的粘附率。

Method : MTT assay was applied to study the effects of different concentration of RGDS peptide on Tb cells ' adhesive capability on Matrigel and Fibronectin .

方法：采用MTT法研究不同浓度的RGDS对Tb细胞粘附Matrigel及Fibronectin能力的影响；

Results The direct adhesive forces of TEC to Matrigel decreased under renal ischemia and anoxia than that under normal condition ( P < 0 . 001 ) .

结果模拟缺血缺氧时肾小管上皮细胞与人工基底膜之间黏附力较正常时明显降低（P＜0.001）。

Objective To measure the adhesive forces of renal tubular epithelial cells ( TEC ) to artificial basement membrane ( Matrigel ) directly under mimic renal ischemia and anoxia .

目的直接测定模拟缺血缺氧时肾小管上皮细胞－基底膜黏附力。

In order to investigate the factors which could enhance the integrity of the biological seal between implant and epithelial cells , basement membrane matrigel was coated on the surface of smooth pure titanium .

为探讨增进牙龈上皮细胞在钛表面附着的因素，将基底膜提取物Matrigel涂在光滑纯钛片表面，扫描电镜观察原代培养的人牙龈上皮细胞在涂Matrigel钛表面和光滑钛表面的伸展情况。

The collagen mixed with Matrigel obviously changed the surface structure of collagen . The surface of the composition became loose with bigger pore size . Collagen fibers were woven into the complex network structure .

而胶原中掺入Matrigel后明显改变了胶原的表面结构，复合物表面变得疏松，孔径变大，胶原纤维交织成网状结构。

The other part of brain glioma stem cells were planted on the Matrigel glue , and celastrols of different concentrations were added like the front steps . We observed whether tubular structures could form .

另一部分脑胶质瘤干细胞种植于Matrigel胶上，重复以上步骤加入不同浓度雷公藤红素，观察转分化过程中有无小管样结构形成。

Results CD34 + cells were able to be induced into endothelial cells , which are CD34 + , CD31 + and stain for ⅷ∶ Ag , and formed capillary structure in the Matrigel .

结果细胞经诱导可转化生成血管内皮细胞，Ⅷ∶Ag、CD31+染色阳性，并可在重组基底膜（Matrigel）中形成三维毛细血管网状结构。

In Boyden Chamber experiment , the quantity of the A549 cells breaking through the matrigel cell increased ( P < 0.01 ), while the quantity of PAa was too little to be counted .

Boyden小室实验中，A549细胞透膜数显著增加（P<0.01），而PAa细胞因透膜数很少，均无法计数。

Methods Using modified Boyden chamber migration assay and short-term 2 - ( dimensional ) culture model on Matrigel , the effect of low dose VBL on ECV-304 cells migration and tubular morphogenesis was evaluated in vitro .

方法分别用改良的Boyden室迁移测试法和Matrigel上血管新生的短期二维培养模型来评价低剂量VBL对ECV-304细胞迁移及管样结构生成的作用。

They were sterilized by dipping in iodine and ethanol , washed with 0.1 % PBS . Subsequently , ultraviolet radiation for 30 min after coated PGA with type I collagen and Matrigel Matrix .

碘酒、乙醇浸泡消毒，0.1%PBS冲洗，Ⅰ型胶原和Matrigel混合物包被，紫外线消毒30min。

Methods The effect of celastrol on the in vitro proliferation of endothelial cell of vessel ( ECV ) was examined by MTT assay . The effect of celastrol on endothelial cell migration , tube formation on Matrigel and Chick chorioallantoic membrane angiogenesis was also examined .

方法采用MTT法测定雷公藤红素对血管内皮细胞株（ECV）增殖的影响，观察雷公藤红素对ECV迁移实验和小管形成实验的作用，以及对鸡胚尿囊膜血管生成的影响。

Results GSCs can be differentiated into VECs in vitro and express endothelial cells special marker CD31 ( + ) and CD34 ( + ) . It can form tuber-like structure in Matrigel medium and secrete NO witch displayed part function of mature endothelial cells .

结果GSCs在体外可以被诱导向VECs分化，表达内皮细胞特定标志物CD31（+）、CD34（+），在基质胶中可以形成血管样结构，可以分泌一氧化氮，具有部分成熟内皮细胞功能。

Methods S-180 tumor imaging and autoradiography of angiogenesis on Matrigel model was developed with 99 Tcm-recombinant human angiostatin ( 99 Tcm-rhAS ) as a tracer , and FCM on human microvascular endothelial cell-1 ( HMEC-1 ) with FITC-rhAS or rhAS antibody .

方法以99Tcm-重组人血管抑素、FITC-重组人血管抑素和FITC-重组人血管抑素抗体为示踪分子，进行小鼠S180肿瘤模型显像、Matrigel血管模型放射自显影和HMEC-1细胞的流式细胞术。