psti

Here we have a research on the influence of PstI restriction endonuclease on the host genomes .

本论文是以研究PstI限制性内切酶对宿主基因组的影响为目的。

PSTI and TTR might be the potential tumor marker candidates . This proteomic study suggest some kind of correlation between inflammatory and tumor pathogenesis .

胰腺癌患者胰液中特异表达的蛋白质PSTI和TTR可能是胰液肿瘤标志物的侯选，本研究提示了慢性炎症与肿瘤发生机制可能联系的相关依据。

By computer analyses , a PstI site was found within the known 5.8S rRNA sequence of the silkworm Attacus ricini .

利用计算机分析已知的蓖麻蚕（Attacusricini）5.8SRRNA顺序，发现其上有一个PstⅠ位点。

This study had found that PstI restriction endonuclease could digest the host genomes which would be degraded by DNase latter , and attained bacteria shell without genomes finally .

经研究发现产生的PstI内切酶可以将宿主基因组切开，被切开的基因组随后会被DNA酶降解，最后可以得到没有基因组的细菌空壳。

The G and N gene of rabies virus SAD-B19 strain were amplified by PCR , the target genes were collected and digested by Confine digestion enzyme of PstI .

通过PCR扩增狂犬病病毒SAD-B19株的G、N基因。将回收的目的片段用限制性核酸内切酶PstI进行酶切。

PstI cleaves its substrate through the single strand cleavage with two steps , and the minimum PstI substrate is an eight to twelve oligodeoxynucleotide containing ist recognition sequence , and there is a requirement to the base pair numbers on both sides of Pst ⅰ recognition sequence ;

PstI切割底物是按两步单股切割机制进行，作用的最小底物是含有识别序列的8～12聚脱氧核糖核苷酸，且对底物中识别序列两边的碱基对数目有一定要求；

We have obtained 662 markers using 19 PstI / Tru1I ( Mse1 ) primer pairs and 4 EcoRI / Tru1I primer pairs , of which 43.2 % has 3:1 in segregation , 14.5 % appears to be non-parental heteroduplex , and 48.8 % is distorted .

选择扩增使用了19对PstI/Tru1I（Mse1）引物和4对EcoRI/Tru1I引物。共得到标记662个，其中3：1标记占43.2%，异倍标记14.5%，偏分离标记（P<0.05）占48.8%。