recombinant DNA technology

Construction of an Amylolytic and Ethanol-producing Yeast Strain by Recombinant DNA Technology

用基因重组技术构建可降解淀粉和产生酒精的酵母工程菌

Method : Adopting the recombinant DNA technology , the prokaryotic expression plasmids of one normal recombinant and tree single-residue variants of bovine prion protein were constructed .

【方法】应用DNA重组技术，正确地构建牛朊蛋白基因的1个正常重组朊蛋白和3个单氨基酸变异体的原核表达载体，经转化E。

With the development of recombinant DNA technology and expression system , quantity of known protein increases .

随着重组DNA技术的发展和表达体系的完善，已知的蛋白质数目有了很大增长。

Application of recombinant DNA technology in entomology

重组DNA技术在昆虫学中的应用

Nesiritide is a synthetic form of brain natriuretic peptide using recombinant DNA technology .

奈西立肽是利用重组DNA技术得到的合成型人类脑利钠肽。

Biochip technology is a great breakthrough of life science after recombinant DNA technology and polymerase chain reaction .

生物芯片技术是继基因工程（重组DNA技术）、聚合酶链式反应（PCR）之后在分子生物学领域中的又一重大突破。

In this study , recombinant DNA technology was applied for producing recombinant human BMP-4 mature peptide .

因此本研究利用基因工程生产人BMP-4成熟肽。

As recombinant DNA technology is consummating increasingly , genetic engineering drug is frequently researched and utilized .

随着重组DNA技术的不断完善，基因工程药物的研制和利用日益增多。

Recombinant DNA technology , transgenic technology is also a matter of fact in the molecular level to create the mosaic .

重组DNA技术、转基因技术事实上也都是在制造分子层次上的嵌合体。

The advent of recombinant DNA technology has provided efficient and cost-effective alternate sources for many therapeutic proteins .

重组DNA技术为许多治疗性蛋白的生产提供了一种有效的替代方法。

The successful cloning of an opioid receptor gene family has made it possible to use recombinant DNA technology at the receptor level .

阿片受体克隆成功打开了将重组DNA技术运用到受体水平的通路。

DNA is the most important genetic material . So the method and technique of DNA extracting is the basic method for biochemistry , molecular biology and recombinant DNA technology .

DNA是最重要的遗传物质，因此，DNA的提取方法和技术是生物化学及分子生物学最基本的技术，也是生物工程最常用的技术。

Before recombinant DNA technology enabled pharmaceutical companies to manufacture human insulin in the1980s , pig and cow insulin were routinely given to diabetic patients .

在1980年重组DNA技术出现使得药厂制造人类胰岛素出现以前，猪及牛的胰岛素已经成为为糖尿病患者治疗常规使用的药物。

Certain APIs of low molecular weight , such as antibiotics , amino acids , vitamins , and carbohydrates , can also be produced by recombinant DNA technology .

有些低分子量的原料药，如抗生素、氨基酸、维生素和糖类也可以用重组DNA来生产。

However , phage-resistant starter cultures were traditionally obtained by recombinant DNA technology or conjugal transfer of plasmids confering phage resistance , which holded health potential dangers .

然而，噬菌体抗性菌株通常是通过DNA重组或携带噬菌体抗性基因的质粒的转移获得的，因而赋予所得菌株潜在的安全隐患。

Although we are now able to produce pharmacological peptides by recombinant DNA technology , yet we fail to produce amidated peptides in the same way because bacteria lack the a-amidase .

尽管我们能够通过重组DNA技术利用细菌生产具有药理活性的蛋白质，然而这种技术却不能用于生产酰胺化多肽，因为细菌中没有α-酰胺化酶。

This paper introduced the expression of BMP in Pichia Pastoris using recombinant DNA technology , determined the phenotype of X - 33 / pPICZ α A - BMP , optimized the growth conditions and expression conditions .

文章介绍了利用基因工程技术在酵母菌中表达BMP的方法，对构建的Pichiapastoris酵母工程菌株X-33/pPICZαA-BMP进行了发酵表达，确定了菌株的表型、高密度生长及表达条件。

Although the traditional amino acid producing methods such as chemical synthesis and isolation from natural sources are still utilized , the most significant trend in amino acid production technique is the swift and violent development of multiple method and biological synthesis , especially the application of recombinant DNA technology .

生物资源提取、化学合成、生物合成和综合法是生产氨基酸的4种技术，目前的发展趋势为生物合成和综合法，特别是将现代生物工程技术应用于氨基酸生产。

Methods : An artificial egf gene was constructed with recombinant DNA technology and inserted into pQE-40 to get a expression plasmid pQE-EGF , in which egf was fused with 6 × His tag . Plasmid pQE-EGF was transformed into E.

方法：用DNA重组技术构建EGF基因并插入表达载体pQE-40，与载体上的6×His标签融合，获得的表达质粒pQE-EGF转化E。

Sensitive assays require a highly purified enzyme with a high turnover number , and a low detection limit for the reaction product . Diagnostic enzymes produced by recombinant DNA technology , which increased yield and quality of enzyme and simplified preparing process , improved the development of EIA greatly .

利用基因工程生产诊断用酶，使酶的纯度大大提高，产量及质量均较自然来源酶好，简化了制备程序，大大促进了酶免疫分析技术的发展。

Prophylactic vaccines based on the use of virus-like particles ( VLPs ) obtained from auto-assembly of L1 or L1 and L2 proteins produced by recombinant DNA technology are under phase I / II clinical trials for HPV6 / 11 associated with condylomas .

通过DNA重组技术，基于HPV6/11型L1或L1和L2蛋白自我组装成病毒样颗粒的研究已经进入了I/II期临床实验。

This highly interdisciplinary field is closely associated with both the physical and chemical properties of organic and inorganic nanoparticles , as well as to the various aspects of molecular cloning recombinant DNA and protein technology , and immune-ology .

这种新颖的交叉跨度很高的化学学科与有机／无机纳米粒子的物理化学性质，以及分子克隆，DNA重组，蛋白质技术和免疫学有着非常紧密的联系。

With the development of recombinant DNA and peptide synthesize technology , and advantage in analytical separation technology , more and more proteins and peptides , such as enzyme , activators and inhibitors , poly - and monoclonal antibodies , and various vaccines , are used as pharmaceuticals in clinic .

随着重组DNA和多肽合成技术的发展，更加之分析分离技术的提高，越来越多的蛋白质和多肽，象酶、活性因子、抑制因子、多克隆与单克隆抗体、疫苗，被作为药物应用于临床。

Therapeutic recombinant proteins are produced by the recombinant DNA technology .

重组药用蛋白质是指利用DNA重组技术生产的蛋白质。

Recombinant chymosin represents one of the first successful applications of recombinant DNA technology in the food industry .

重组凝乳酶是重组DNA技术在食品工业中的首次成功应用。