交联蛋白

AGEs交联蛋白裂解剂的研究进展

Research progress of AGEs crosslinked protein breaker

结果：交联蛋白涂层上化学键结合抗DNA抗体的量比单纯物理吸附高约15倍，支架上通过抗DNA抗体免疫偶联质粒DNA的细胞转染效率明显高于单纯物理吸附携带基因的支架。

Results : The amount of chemically coupled antibody on the protein coating stents was fifteen times higher than the physically absorbed control stents , and the transfection efficiency of anti-DNA antibody chemically coupled with plasmid DNA was significantly better than that of the control stents .

链霉菌谷氨酰胺转胺酶是目前唯一商业化的能够交联蛋白的酶制剂。

Streptomyces TGase is the only commercial enzyme that could covalent proteins .

交联蛋白晶体技术及其应用

Cross-linked Protein Crytals Technology and Its Applications

作为蛋白工程的一项突破性技术，交联蛋白晶体技术正受到广泛重视。

As a breakthrough technology in protein engineering , cross | linked protein crystals technology is attracting wide attentions .

结论：①在腺病毒介导下，由交联蛋白涂层金属支架向内皮，中层平滑肌细胞和外膜转基因有效可行且转染效率较高。

Conclusions : Adenovirus mediated arterial gene transfer to endothelial , smooth muscle cells and adventitia is feasible by protein coated metallic stent with a high transfection efficiency .

酶法水解交联蛋白的最终产物是蛋白水解物，已经发现了它们在一些新领域中的应用价值，残余的铬泥处理就是该文要讨论的问题。

End products of enzymatic hydrolysis of a chrome cross-linked protein matrix are protein hydrolysate which is constantly finding new fields of application , and residual chrome sludge whose use is the subject of discussions .

在基于pH的不同肌肉类型和种类中，肌原纤维蛋白能在导致产品差异方面起重要作用。通常地，出现的共价交联在蛋白的结构和功能关系中起重要作用。

It plays a significant role in causing product variations among different muscle types and species based on the pH. Naturally occurring covalent crosslinks play an important role in the structure-function relationships of proteins .

尽管它们在体内的半衰期很短，但可以与DNA、蛋白质和多元不饱和脂肪酸作用造成DNA链断裂和氧化性损伤、蛋白-蛋白交联、蛋白-DNA交联和脂质过氧化。

Though radicals has a very short time of half-life , it can react with DNA , protein and PUFA , which can make DNA chain rupture and damage , coupling of protein-protein , coupling of protein-DNA and fat anti-oxidation .

D-二聚体（D-dimer）是交联纤维蛋白降解后形成r′-r′链的纤维蛋白特异性降解产物，为纤维蛋白降解产物中的最小片段[1]。

D-dimer is a specific degradation product of fibrous protein of the r ′ - r ′ chain formed after the degradation of cross-link fibrous protein . It is the smallest fragment of the degradation product of fibrous protein .

将交联纤维蛋白胶体置于pH7.6的TBS缓冲液中，加入纤维蛋白溶解酶。经过37℃摇床水解48小时，交联纤维蛋白胶体基本完全降解为可溶性降解产物。

The cross-linked fibrin gel was placed into pH7.6 TBS buffer , adding fibrinolytic enzymes , after shaking at 37 degrees Celsius for 48 hours , cross-linked fibrin almost completely degraded into soluble degradation products .

小鼠及人源化鼠抗人交联纤维蛋白单链抗体的表达与纯化

Expression and purification of mouse and humanized mouse ScFv specific against human crossing linked fibrin

通常地，出现的共价交联在蛋白的结构和功能关系中起重要作用。

Naturally occurring covalent crosslinks play an important role in the structure-function relationships of proteins .

结论：胆石形成前即有凝血活化和交联纤维蛋白形成。

CONCLUSIONS : Coagulation cascade is activated and crosslinked fibrin forms in gallbladder bile before gallstone formation .

尿激酶抗人交联纤维蛋白-D-二聚体单抗化学偶合体的制备

Preparation of Conjugate of Urokinase With Monoclonal Antibody against Fragment D dimer of Human Cross linked Fibrin

目的：克隆和表达人源化小鼠抗人交联纤维蛋白单链抗体。

Objective : To clone and express humanized mouse ScFv against human cross linked fibrin ( HScFv ) .

经尿素溶解实验确定为交联纤维蛋白胶体。

After determined by the urea dissolution experiments , it was confirmed cross-linking of fibrin gel . 2 .

它不但能直接作用交联纤维蛋白，而且还可激活体内的纤溶酶原，从而表现出很强的溶血栓作用。

It could directly cross-link fibrin , but also activate plasminogen in vivo , thus demonstrating a strong thrombolytic effect .

D-双聚体和E片段均为交联型纤维蛋白的降解产物。

The D - Dimer and E fragment are degradation products of cross - linked fibrin .

PC交联的弹性蛋白支架具备良好的血液相容性、抗血栓和抗炎潜能，满足了组织工程带瓣管道的关键要求。

In addition , PC-crosslinked aortic elastin displays excellent hemocompatibility , anti-thrombus and ant-inflammatory potential , fulfilling the key requirements of tissue-engineering scaffold for valved conduit .

采用经EDC交联的纤维蛋白凝胶作为可注射性软骨细胞支架材料的研究

Fibrin Glue Cross-Linked with EDC as Injectable Scaffold for Cartilage Engineering

其表面都包被着一层蛋白膜，或单独地或相互交联分散在蛋白基质中。

They were all covered with a layer of protein film and dispersing in the protein matrix individually or by a coalescent way .

基因工程抗体融合蛋白比传统的化学交联的抗体融合蛋白具有更多的优越性。

Genetically engineered antibody fusion proteins have more advantages over traditional chemical crosslinking antibody fusion proteins .

结果表明，用丝胶和阳离子交联剂对大豆蛋白纤维改性，织物的性能明显改善。

The results showed that the soybean protein fiber properties modified by sericin and cationic agent were improved greatly .

已经有文献报道过酶化改性后的具有交联特性的各种蛋白用于食品工业中。

It has been established in the literature that a variety of proteins used in food products are polymerized by the crosslinking properties of enzymatic modification .

循环系统里的成熟红细胞在其生命过程里经历着氧化性衰老，相应细胞组份出现血红蛋白变性、膜蛋白交联、带3蛋白聚集以及带3蛋白降解等多种修饰。

Mature erythrocytes in the circulatory system experienced oxidative aging , modified with hemoglobin denaturation , membrane protein crosslinking , and Band 3 clustering and degradation .

利用阳离子交联剂DE，将丝胶蛋白固着在大豆蛋白纤维上，确定了DE和丝胶交联改性大豆蛋白纤维的较优工艺。

Cationic crosslinker DE was used to cure sericin on soybean protein fibers , and an improved process for this combined modification was detd .