免疫沉淀

染色质免疫沉淀技术在研究DNA与蛋白质相互作用中的应用

Application of Chromatin Immunoprecipitation Assay in Deciphering DNA-Protein Interactions

并利用免疫沉淀和WESTERNBLOTTING技术检测Bcl-2融合蛋白的表达。

The expression of bcl-2 fusion protein was detected with the immunoprecipitation and Western blotting technology .

放射免疫沉淀试验鉴定单纯疱疹病毒糖蛋白C

Identification of glycoprotein C of herpes simplex virus

采用免疫沉淀法对187例乙肝病毒感染患者作了DNA聚合酶（DNA&P）的测定。

Enzyme activity of HBV DNA polymerase ( DNA-P ) in 187 cases with HBV infection was assayed with immune sedimentation method .

疑似鼠疫患者放射免疫沉淀试验F1抗体阳性与讨论

Discussion about the Suspectable Patients of Plague with RIP F1 Positive

用免疫沉淀试验检测单克隆抗体的Ig类型为IgG1。

Using immuno-preciptation the author determined that the Ig was of IgG_1 type .

运用免疫沉淀技术（IP）检测MDM2蛋白的磷酸化水平；

Immunoprecipitation ( IP ) was conducted to examine the phosphorylation level of MDM2 protein .

采用双抗体免疫沉淀技术分离和提纯大鼠肝细胞白蛋白mRNA

Isolation and purification of rat liver albumin mRNA by double antibody immunoprecipitation technique and poly ( u ) - sepharose affinity chromatography

免疫沉淀结果表明，c-Jun与AR存在蛋白-蛋白相互作用。

Furthermore , physical interaction of c-Jun and the AR was confirmed by coimmunoprecipitation result .

双抗体免疫沉淀法纯化牛生长激素poly（A）~+RNA的研究

A Study on the Isolation and Purification of Bovine Growth Hormone Poly ( A ) ~ + RNA by Immunoprecipitation

方法应用WESTERNBLOTTING，免疫沉淀、激酶活性测定和RTPCR方法检测37例人乳腺癌及其周围正常组织的Erk2表达及活性变化。

Method Western blotting , immunoprecipitation , kinase activity assay and RT-PCR were used on 37 cases .

免疫沉淀法纯化蛋白并用ERK1/2试剂盒测定ERK活性；

ERK activity was measured by ERK1 / 2 immuno-precipitation kinase assay kit ;

放射免疫沉淀试验检查鼠疫FI抗体诊断标准的研究

A Study of Diagnostic Criterion of RIP to Detection of Antibody FI of Plague Bacillus

植物叶片中Rubisco含量的免疫沉淀法测定

Measurement of Rubisco content in plant leaves by immunoprecipitation assay

应用生物素标记细胞膜分子、免疫沉淀及化学发光测定p140的Mr。

Biotin labeling , immunoprecipitation and chemiluminescence were used for identification of Mr of this molecule .

染色质免疫沉淀法是研究染色质结构的首选方法，它不仅可用来研究体内反式因子与DNA的相互作用，也可以用来研究组蛋白修饰与基因表达的关系。

Chromatin immunoprecipitation assay ( ChIP ) provides a powerful tool to analyze the interaction of trans-acting factors with specific chromatin regions in vivo , as well as the role of histone modifications in gene regulation .

用WESTERNBLOTTING分析、代谢标记和免疫沉淀测定、电镜观察心肌细胞Cx43表达、合成和间隙连接。

The Cx43 expression , synthesis and gap junction in cardiomyocytes were characterized by Western blotting , metabolic labeling and immunoprecipitation assay , and electron microscope .

蛋白免疫沉淀和酶自身磷酸化率法测定c-Src激酶活性；

C-Src kinase activity was measured by protein immunoprecipitation and kinase autophosphorylation .

染色质免疫沉淀（ChromatinImmunoprecipitation）分析是近年发展起来检测蛋白因子在体内与染色质的结合，进而为蛋白因子参与染色质水平基因转录调控提供证据的方法。

Chromatin Immunoprecipitation ( ChIP ) assay was developed in recent years to detect protein factors that bind to chromatin .

与其他方法相比，染色质免疫沉淀技术（ChromatinImmunoprecipitationAssay，ChIP）是一种在体内研究DNA-蛋白质相互作用的理想的方法。

Although many techniques can be used for this purpose , chromatin immunoprecipitation assay ( ChIP ), by contrast , is ideally suited for studying DNA-protein interactions in vivo .

方法：以抗ATM抗体免疫沉淀的ATM为激酶，抗HA抗体免疫沉淀的PTC1的TK结构域为底物，进行体外磷酸化，检测ATM对ret-TK结构域的磷酸化作用；

METHODS : RET TK phosphorylation was determined by in vitro kinase assay using the immunoprecipitation with anti ATM antibody as kinase and the immunoprecipitation of HA tagged TK as substrate .

采用免疫沉淀、薄层层析及γ液闪计数方法检测PI3K的活性，并进行分析比较。

PI-3 kinase activity was detected by immunoprecipitation , thin-layer chromatography and gamma scintillation counting . The results were analyzed by statistical methods .

用免疫沉淀及免疫印迹方法鉴定mAb对重组TRAIL（RecombinantTRAIL，rTRAIL）和细胞裂解液中TRAIL分子的识别。

Its recognition to recombinant TRAIL ( rTRAIL ) and native TRAIL in cell lysate was identified by immune precipitation ( IP ) and western blot assay .

然后，抗一种特异TF或DNA-结合蛋白质的一种抗体被用来免疫沉淀特异的DNA-TF复合体。

An antibody against a specific TF or DNA-binding protein is then used to immunoprecipitate specific DNA-TF complexes .

免疫沉淀和Western印迹分析结果表明，缺血区ICAM－1的表达在大脑中动脉栓塞后3h增高，6～12h达到高峰，并持续到72h。

As judged by Western blot analysis , ICAM 1 expression in the ischemic hemisphere was increased at 3 h following MCAO , peaked at 6 to 12 h , and persisted 24 to 72 h.

微量注射mRNA于蛙卵母细胞。72h后以~3H&Leu参入值测定蛋白质合成增加，并以免疫沉淀方法证明了mRNA注射蛙卵产物具有抗甲状腺球蛋白抗体的免疫活性。

Micro-injection of the mRNA into frog oocyteS resulted in increasing of protein synthesis detected by incorporation of 3H-Leu . the immunological activity of antibodies against thyroglobulin was proved in the products of oocytes after mRNA injection by immuno - precipitation .

用抗磷酸酪氨酸单抗或抗NR2A抗体以免疫沉淀法和免疫印迹法检测海马NR2A酪氨酸磷酸化水平。

Tyrosine phosphorylation of NR2A in hippocampus was assessed by immunoprecipitation and immunoblotting using anti-phospho-tyrosine antibody and antibody to NR2A .

HE染色检测心肌组织病理变化，免疫沉淀法检测ERK1/2蛋白表达、CaN蛋白表达及磷酸化、以及αskeletalactin蛋白表达，RTPCR检测心肌球蛋白重链（βMHC）mRNA表达。

Immunoprecipitation was used to assay the protein expression and phosphorylation of CaN and ERK1 / 2 , and the protein expression of α - skeletal-actin in myocardial tissues . The mRNA expression of β - myosin heavy chain (β - MHC ) was measured by RT-PCR .

放射免疫沉淀实验结果表明，这6株单抗均识别登革2型04株病毒的42K非结构性多肽。

Radioimmune precipitation test shows that the six McAbs are against M.W. 42 K dolton nonstructure protein of D-2 04 virus .

此外两种因子均诱导与酪氨酸磷酸化过程密切相关的src基因表达，免疫沉淀证实CTGF结合细胞表面受体后可诱导细胞内相应蛋白的酪氨酸磷酸化。

On the other hand CTGF and FGF both can induce src gene expression and the result of the immunological precipitation with anti-phosphotyrosine antibody suggested that tyrosine phosphorylation of a protein in KMB-17 perhaps is related with the signal transduction of CTGF .