凝血酶

高甲硫氨酸诱发高同型半胱氨酸血症对抗凝血酶-Ⅲ，蛋白C和血管性血友病因子的影响

Effects of methionine-induced hyperhomocysteinemia on protein C , antithrombin - ⅲ and von willebrand factor

凝血酶受体活化与血小板膜糖蛋白Ⅰbα的异位分布机制

Functions of thrombin receptors in the reversible distribution of platelet surface glycoprotein ⅰ b α in activated platelets

凝血酶经CM离子交换液相层析柱进一步纯化。

Thrombin was further purified with CM ion-exchange liquid chromatographic column .

凝血酶对血管内皮细胞核因子κB活化及基质金属蛋白酶2表达的影响

The Role of Nuclear Factor - κ B in the Thrombin Induced Expression of Matrix Metalloproteinase-2 in Human Umbilical Vein Endothelial Cells

凝血酶致大鼠肺微血管通透性及血浆VEGF含量改变的实验研究

Changes of VEGF expression and pulmonary microvascular permeability by thrombin injection in rats

第二部分：利用金纳米粒子标记的DNA适配子为探针测定凝血酶的共振光散射分析。

Part two : Study on the Resonance Light Scattering analysis to determine thrombin by using gold nanoparticle-labeled DNA aptamer as probe .

目的研究凝血酶调节蛋白（TM）N端155个氨基酸残基的原核表达。

Objective To express the protein of the lectin - like domain of thrombomodulin ( TM ) .

方法凝血因子活性测定采用一步法检测血浆FⅦ：C活性水平及相应的凝血酶元时间（PT）。

Methods One-step method was used to detect the levels of plasma factor ⅶ activity and relevant prothrombin time .

纤维蛋白原（Fbg）的活性测定采用凝血酶法；

Plasma fibrinogen ( Fbg ) was measured with thrombin assay .

葛根素对凝血酶诱导的血管平滑肌细胞增殖及凝血酶受体mRNA表达的影响

Effects of puerarin on thrombin-induced-proliferation of vascular smooth muscle cells and the role of puerarin on the TR mRNA expression

凝血酶受体激活肽和凝血酶对人成纤维细胞释放TGF-β和VEGF的影响

Effects of thrombin peptides and thrombin on the release of TGF - β and VEGF from human fibroblasts

结果表明：（1）0.4～3.2NIHu／ml凝血酶显著促进GEC的增殖和代谢，P＜0.05；

0.4 3.2 NIH U / ml thrombin promoted GEC proliferation significantly ( P < 0.05 ) .

核因子&κB在凝血酶诱导平滑肌细胞增殖中的作用及其反义RNA的干预研究

Nuclear Factor & κ B 's Role in Proliferation of Vascular Smooth Muscle Cell Induced by Thrombin and the Intervention of Its Anti-sense RNA

腺病毒介导的凝血酶敏感蛋白1抗血管新生衍生物基因转移抑制K562细胞裸鼠体内生长

Adenoviral vector expressing an antiangiogenic fragment of thrombospondin 1 inhibits the growth of K562 cell xenografts

脑出血后血浆凝血酶及MMP-9的动态变化及相关性

The Dynamic Changes of Thrombin and MMP-9 in Plasm and the Relevance between Them after Intracerebral Hemorrhage

结论GPIb是血小板上的一种重要的凝血酶受体。

Conclusion GPIb is an important thrombin receptor on platelet .

凝血酶致大鼠脑出血后脑水肿及脑内MMP-9、MMP-2表达的实验研究

Experimental Study of Brain Edema and Expression of MMP-9 and MMP-2 after Intracerebral Hemorrhage Induced by Thrombin

分析PA的形状、大小、颈部长度与宽度、凝血酶剂量、治疗结果以及并发症。

PA form , size , neck length and width , thrombin dose , therapic effect and complications were analysed .

凝血酶上调人肾小球系膜细胞PAI-1表达的细胞内信号转导研究

Study on intracellular signaling transduction pathways of plasminogen

结论超声引导凝血酶注射治疗股动脉PA安全有效，可作为经股动脉途径介入诊疗术所致医源性PA的首选治疗。

Conclusion : UGTI is safe and effective as the first-line therapy of iatrogenic PA following femoral arterial puncture for PCI .

携带反义凝血酶受体和p21双基因共表达腺病毒伴随病毒载体的构建与包装

Construction and Package of Antisense Thrombin Receptor and p ~ ( 21 ) Co-expression Adeno-associated Virus Vector

减少大鼠离体肠系膜上动脉ET＿1的基础释放及凝血酶刺激的ET1释放。

And PGI_2 reduced basic ET-1 release and thrombin-induced ET_1 release from isolated mesenteric artery .

结果：凝血酶导致脑组织水肿，引起脑组织病理学改变。其作用高峰期为24-72小时（P＜0.05）。

Results : Thrombin caused an increase in brain water content and pathologic change of brain tissue ( P < 0.05 ), this effect began at 12 hours and peaked at 24 to 72 hours .

凝血酶时间（TT）在4,24及48h均表现为显著缩短（P<0.05）；

The thrombin time ( TT ) was shorter at all the three time points ( P < 0.01 );

体外聚集活性测定结果显示，冰冻干燥血小板保存不同温度和时间，其凝血酶诱导下的聚集活性与新鲜血小板相比，无显著差异（P0.05）。

The result of in vitro aggregation suggests that lyophilized platelets preserved at different temperature and stored for different period shows no significant difference with the fresh platelets .

本实验的目的是采用载体两性电解质pH梯度等电聚焦电泳的方法，结合SDS-PAGE测定牛凝血酶的等电点。

The purpose of the experiment is to assay the isoelectric point of bovine thrombin by carrier ampholytes pH gradient isoelectrofocusing ( IEF ) electrophoresis and SDS-PAGE .

通过SephadexG－100凝胶层析，测出猪凝血酶的分子量为30kd。

The molecular weight of thrombin was 30 kd determined by Sephadex G 100 gel filtration .

方法：凝固法测凝血酶原时间（PT），活化的部分凝血活酶时间（APTT），凝血酶时间。

Methods : coagulate determined prothrombin time ( PT ), activated partial thromboplastin time ( APTT ) and thrombin time ( TT ) .

在体外培养条件下观察了凝血酶对人肾小球系膜细胞表达细胞外基质降解酶&明胶酶A（MMP－2）及明胶酶B（MMP－9）的调节作用。

Cultured human glomerular mesangial cells were used to evaluate the effects of thrombin on its expression of gelatinase A ( MMP2 ) B ( MMP9 ) .

方法：家兔小脑延髓池注射凝血酶和纤维蛋白原复制NPE模型。

METHODS : NPE was made with intracisternal injections of fibrinogen and thrombin .