分子量标准

一种用于形成1kb阶梯DNA分子量标准载体的构建

Construction of Vectors for Creating 1 kb Ladder DNA Molecular Weight Standards

DNA分子量标准制备技术：方法与进展

Preparation of DNA Molecular Weight Standards : Methods and Advances

一种新型可用于DNA分子量标准的质粒构建

Construction of A New Kind of DNA Marker Vector

通过文献检索和比较，选择3%浓缩胶和10%的分离胶作为电泳胶板，以低分子量标准蛋白为marker电泳。

3 % of concentration gel and 10 % of separation gel was chose as electrophoresis plastic through literature search and comparison , low-molecular-weight standard protein as marker for electrophoresis .

分子量标准引物定位合成（MarkerPrimer-directedSynthesis，简称MPDS）乃一种DNA碱基对梯度片段的简易合成术。

Marker primer-directed synthesis ( MPDS ) is a technique that allows the easy synthesis of DNA base pair ( bp ) ladders .

用m田火er作为分子量标准，p一actin作为内对照，皮肤组织标本代替实验标本作为阴性对照。

Use marker as the standard of molecular weight , and | 3 - actin as internal control , and skin tissue sample as negative control instead of experimental sample . 5 .

葡聚糖分子量标准物质的研制

Development of Dextran Molecular Weight Certified Reference Materials

建立高效的的蛋白质分子量标准生产体系对于蛋白标准品的生产和商业化有重要价值。

Efficient production of protein molecular standard is crucial to commercialization of protein standard .

一种分子量标准的引物定位合成

Primer-directed Synthesis of a Molecular Weight Marker

在建立了激光光散射定值分析方法基础上，研制了3种葡聚糖分子量标准物质。

Based on the establishment of light scattering value determination method , three dextran molecular weight CRMs are studied and characterized .

采用蛋白质分子量标准物和纯化的重组蛋白质进行半干式蛋白质印迹，探讨半干式电泳印迹的多种因素对印迹效果的影响。

To elucidate the effect of the semi-dry electrophoretic transfer and its influencing factors in Western blot , the protein marker and purified recombination protein were used to analyzed .

同一蛋白质斑点在3次实验中等电点、分子量的相对标准差分别为8.76%±5.14%，13.00%±4.22%和10.84%±9.16%。

The isoelectric point and molecular weigh of the same protein spots in three times experiments deviates from relative standard as following : 8.76 % ± 5.14 % , 13.00 % ± 4.22 % and 10.84 % ± 9.16 % respectively .

方法：采用SDS-PAGE以及银氨染色法检测嗜人按蚊中肠膜蛋白分子量，并对分子量标准（Mr116000～14400）进行直线回归分析。

Methods : The membrane protein was analyzed by SDS-PAGE and silver staining method and the standard molecular weight ( Mr 116 ~ 14.4 kD ) was made by the linear regression .