末端补平
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- end-filling
末端补平-
方法：将环化质粒以限制性内切酶消化成线性后，以Klenow大片段将末端补平，再用taqDNA聚合酶在线性载体的两个5′末端各加上一个非配对的T。
Methods : The circled TA plasmid was digested and linearized with restriction endonuclease . The ends of the plasmid were repaired and made blunt with Klenow Fragment . A single T was added respectively to the 5 ′ ends of the vector with Taq DNA polymerase .