副溶血弧菌

食品中副溶血弧菌荧光定量PCR方法快速检测

Rapid detection of Vibrio parahaemolyticus in food by fluorescence quantitative PCR

副溶血弧菌的多重PCR检测

A Multiplex PCR Method to Detect the Vibrio parahaemolyticus Strains

实时荧光定量PCR法与常规PCR法及细菌培养法检测副溶血弧菌的比较

Comparison among real-time PCR , PCR and bacterium culture in detection of Vibrio parahaemolyticus

海产品中副溶血弧菌PCR检测方法的建立与评价

Development and Evaluation of PCR Method for the Detection of Vibrio parahaemolyticus in Seafoods

添加有扩增内标的副溶血弧菌PCR检测方法的研究

Study on the detection of Vibrio parahaemolyticus using PCR method with an internal amplification control

四种副溶血弧菌总RNA提取方法的比较

Comparison of four Kinds of Methods for Extracting Total RNA from Vibrio parahaemolyticus

金色葡萄球菌、伤寒沙门氏菌和副溶血弧菌单管多重PCR检测方法的建立及初步应用

Establishment and application of multiplex PCR for diagnose Staphylococcus aureus , Salmonella typhi and Vibrio parahaemolyticus

应用斑点ELISA技术检测副溶血弧菌

Detection of Vibrio parahaemolyticus by Dot-ELISA

用PCR方法对分离自浙江省部分地区的副溶血弧菌临床和海产品分离株的3种溶血素基因进行检测。

PCR method was used to examine three different hemolysin genes in isolates from clinical and seafood samples in Zhejiang province .

用副溶血弧菌人工感染对虾的死亡率C组为58.3%，与对照组（33.3%）存在显著差异（P<0.05）。

At same time , mortality ( 58.3 % ) due to Vibrio parahaemolyticus infection in Group C was significantly higher than that in Control Group ( 33.3 % ) .

本文建立了网箱养殖大黄鱼病原菌&副溶血弧菌酶联免疫吸附法，也即间接ELISA快速检测方法。

Presented in this paper is a method of indirect Enzyme Linked Immunosorbent Assay ( ELISA ) for rapid diagnosis of pathogenic V. parahaemolyticus .

双重PCR检测携带有tl和tdh基因的副溶血弧菌毒力菌株

Detection of tl and tdh Genes in Toxigenic Vibrio parahaemolyticus Strains Using Double Polymerase Chain Reactions

食品中副溶血弧菌FQ-PCR快速检测方法的研究

Faster detection of Vibrio Parahaemolyticus in foods by FQ-PCR technique

副溶血弧菌感染12h和24h杂色鲍肝脏全长cDNA文库的构建

Construction of liver full-length cDNA libraries of abalone Haliotis diversicolor infected with Vibrio parahaemolyticus for 12 h and 24 h

副溶血弧菌（Vibrioparahaemolyticus，VP）是一种海水中常见的条件致病菌，可引起海水养殖鱼类疾病。

Vibrio parahaemolyticus is a familiar conditional pathogen bacterium in marine ; it can induce the disease of fish .

TDH和TRH与副溶血弧菌的致病能力关系密切。

TDH and TRH have close relationship with the disease-causing ability of vibrio parahaemolyticus .

菌株HY和HG的生理生化特性分别与嗜水气单胞菌和副溶血弧菌相似。

The physiological and biochemical features of strains HY and HG were similar to that of Aeromonas hydrophila and Vibrio parahaemolyticus , respectively .

副溶血弧菌海产品分离株tdh基因及其临近区域结构分析

Analysis of tdh gene and its adjacent loci of Vibrio parahaemolyticus isolates from seafoods

副溶血弧菌tlh基因的克隆及序列分析

Cloning and sequence analysis of the tlh gene of vibrio parahaemolyticus

与副溶血弧菌（V.parahaemolyticus）热稳定性溶血素基因（TDH）的相似性为74.5%；

The similarities with thermolabile hemolysin genes of Vibrio parahaemolyticus was 74.5 % .

副溶血弧菌（VibrioparahaemolyticusVp）是一种革兰氏阴性嗜盐菌，其引起的疾病，发病率高，流行范围广，危害严重。

Vibrio parahaemolyticus ( Vp ) is gram negative halophilic , leading to some diseases , high incidence , prevalence wide range , serious damage .

副溶血弧菌tlh基因的克隆、表达及功能的研究

Molecular cloning , expression and functions of the tlh gene of Vibrio parahaemolyticus

结果大蒜液对副溶血弧菌作用30min，杀灭率为100%；

RESULTS The killing rate of V.parahaemolyticus in suspension exposed to garlic solution for 30 min was 100 % ;

根据对这2株菌的形态及生理生化特征检测结果，确定这2株菌分别为溶藻弧菌（Vibrioalginolyticus）和副溶血弧菌（V.parahaemolyticus）。

Based on the morphology and the physiological and biochemical characteristics , these 2 bacteria were identified as Vibrio alginolyticus and V. parahaemolyticus , respectively .

分离到对副溶血弧菌有抑制作用的3株乳酸菌，命名为A-1、A-2、A-3。

Three lactobacillus strains isolated from yoghourt were named A-1 , A-2 , A-3 and their metabolic products can inhibit Vibrio parahaemolyticus .

副溶血弧菌菌株1211U的活的非可培养状态

THE VIABLE BUT NONCULTURABLE STATE OF Vibrio parahaemolyticus 1211U

本实验证明了副溶血弧菌1211U株在一定的条件下可进入活的非可培养状态（VBNC）。

So it is concluded that Vibrio parahaemolyticus 1211U can enter the viable but nonculturable state under certain conditions .

目的分别制备抗副溶血弧菌及其不耐热溶血毒素（thermolabilehemolysin，TLH）的多克隆抗体，建立检测副溶血弧菌TLH的酶联免疫吸附试验（ELISA）双抗体夹心法。

Objective To prepare both polyclonal antibodies of anti-Vibrio Parahaemolyticus and anti-thermolabile hemolysin ( TLH ) for establishing the enzyme-linked immunosorbent assay ( ELISA ) double antibodies sandwich method to detect TLH .

近年的副溶血弧菌分子流行病学研究表明，一种新的tdh阳性、trh阴性的O3：K6群副溶血弧菌菌株引起了包括亚洲、美洲等多个国家在内的大流行。

Recently many molecular epidemiology studies revealed that a new O3 : K6 V. parahaemolyticus strains with tdh positive and trh negative have caused a pandemic of gastroenteritis at least in Asia and America .

第20d用副溶血弧菌攻毒，3000、6000mg/kg处理组均显著提高了幼体存活率；

Larvae fed 3000 mg / kg and 6000 mg / kg of LAPP hed significantly higher survival rate than those treated with 600 mg / kg and the control diet after challenged with V.parahaemolyticus .