匀浆器
- 名homogenizer
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肿瘤组织在&60℃连续冻融两次后,用Potter匀浆器匀浆粉碎,进行Ficoll不连续梯度离心,得纯度较高的细胞膜。
Tumor tissue was frozen twice at - 60 ℃ and homogenized by Potter homogenizer . Cell membrane in homogenate was layered and purified by discontinuous Ficoll-gradient centrifugation .
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从剩余胃黏膜组织中精确称取2g,置于玻璃匀浆器中研磨制成组织匀浆,按放免试剂盒说明测定其表皮生长因子含量。
G surplus gastric mucosa was weighed and put into homogenizer to grind into tissue homogenate ; its EGF content was detected according to the Farr test kit .
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方法:应用玻璃匀浆器轻研磨法和0.25%胰蛋白酶4℃消化约4~6h后,获得原头蚴体细胞。
Methods : combing the glass pestle with 0.25 % trypsin at 4 ℃ to digest protoscolices about 4 ~ 6 hours later , the body cells can be obtained .
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结果发现,液氮研磨组分离完整RNA的阳性率高于匀浆器处理组,分别为68.42%和29.29%,两组比较有显著性差异;
The results showed that the positive rates of RNA integrity in grind with liquid nitrogen group and in homogenizer without liquid nitrogen group were 68.42 % and 29.79 % respectively , and there was significant difference between these two groups ;
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为探讨人类胚胎组织完整RNA的分离方法及其影响因素,采用液氮研磨及匀浆器两种制备组织匀浆方法,对不同引产方式的人类胚胎组织进行总RNA分离,并对RNA质量进行鉴定。
To investigate the method of RNA isolation from human embryonic tissues and the factors influencing the quality of RNA , the RNA from human embryonic tissues obtained with drug-induced labor or non-drug induced labor were isolated by using grind with liquid nitrogen or homogenizer without liquid nitrogen .
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你用高速匀浆器在含0.2M蔗糖,pH7.2的缓冲液中使牛心组织匀浆。
You homogenize the beef heart tissue in a high-speed blender in a medium containing 0.2 M sucrose , buffered to a pH of 7.2 .
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以组织匀浆器和超声波破碎螨体,在柠檬酸介质中分离蠕形螨的细胞核。
After smashing the mites by combination of tissue grinder and ultrasonic wave , mites ' nuclei were extracted in citric acid solution .
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方法将从小鼠肌肉分离、纯化的旋毛虫幼虫放入玻璃匀浆器匀浆,胰酶消化。
Methods Larvae of Trichinella spiralis isolated from the muscle of a Trichinella-infected rat were put into a glass homogenizer for homogenizing , and digesting with pancreatin .
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方法:分别采用0.25%胰蛋白酶消化法、玻璃匀浆器研磨法、玻璃匀浆器轻研磨与0.25%胰蛋白酶消化结合法对原头蚴体细胞进行分离。
Methods : The protoscolices were treated by 0.25 % trypsin , the glass pestle , and mixed way which combined the glass pestle with 0.25 % trypsin , respectively , in vitro .