印迹技术

  • 网络Southern blot;Northern blotting;Southern blotting;blotting
印迹技术印迹技术
  1. 采用RT-PCR及Southern印迹技术观察CCK受体在大鼠PIMs内的表达亚型及LPS对其表达的影响。

    The subtypes of CCK receptor and the effects of LPS on its expression were investigated by RT-PCR and Southern blot analysis in rat PIMs .

  2. Western印迹技术在重组人肿瘤坏死因子α衍生物质控中的应用

    Application of Western Blotting Technology for the Quality Control of r-hTNF α Derivatives

  3. 非放射性Western印迹技术改进的研究

    Improvement of technique for non - radioactive Western Blot

  4. 方法:17例食管癌切除标本,采用组织病理学和Western印迹技术研究食管癌和癌旁正常组织及各级病变组织中磷酸化酪氨酸蛋白的变化。

    Methods : The phosphotyrosine protein was determined by histopathological and Western Blot techniques .

  5. 本文报告用Western印迹技术对,r-hTNFαD进行定性分析。

    This paper reports that the qualitative analyses of r-hTNF α D by Western Blotting technology .

  6. 分子印迹技术选择吸附去除水中Cu(Ⅱ)离子的研究

    Molecular imprinting technique for selective adsorption of Cu (ⅱ) in water

  7. 采用狭缝杂交和Northern印迹技术鉴定差异基因片段;

    Slot blot and Northern blot were used for the expression pattern analysis of the genes .

  8. 应用Southwestern印迹技术和原位杂交技术对大鼠DNA结合蛋白的研究

    Study on rat DNA binding protein by Southwestern blot and in situ hybridization techniques

  9. 蛋白印迹技术检测线粒体和细胞质细胞色素c、总Bax,Bcl-2及p53表达;

    P53 and Bax , Bcl-2 expressions were detected by Western blotting and RT-PCR .

  10. 鉴定IgEBF/sCD23的IgE介导的间接Western印迹技术的建立

    Establishment of IgE mediated Western blotting in identifying IgE BF / sCD23

  11. 本文利用分子印迹技术分别制备铜(II)离子和苯胺的分子印迹聚合物,并利用它们分别选择吸附去除水中铜(II)离子和苯胺。

    This paper aimed to remove Cu ( II ) and aniline in water by molecular imprinting technique .

  12. 用免疫印迹技术证明:在割胶收集的胶乳中肌动蛋白主要分布在C乳清(乳管细胞的细胞质)中;

    Camel Milk By using Western-blotting , actin was found mainly in C-serum ( cytoplasm of laticiferous cells ) of the latex collected by exploitation .

  13. 应用RNA印迹技术和流式细胞仪检测分析全肺的基因(表达)。

    Gene profiling of whole lungs was performed , followed by Northern blot and flow cytometric analysis .

  14. 淋巴瘤为恶性克隆起源的血液病,Ig基因的重排测定可以帮助其分型和预示早期复发.本文用Southern印迹技术分析人9种恶性淋巴瘤细胞系,并于正常胚系DNA比较。

    Nine kinds of malignant lymphoma cell lines and normal germline DNA using southern blotting technique were compared .

  15. Western印迹技术研究神经管发育和神经管畸形鼠胚Bcl-2蛋白的表达

    Western blot technique for the development of neural tube and the expression of Bcl-2 protein in mouse embryo with neural tube defect

  16. 同时,采用免疫印迹技术测定SLE壮族患者自身抗体谱。

    Meanwhile , the autoantibodies of SLE patients were detected by immunoblot technique .

  17. 方法以牛舌粘膜作为底物,分别应用间接免疫荧光法和免疫印迹技术检测16例天疱疮患者血清中IgG型自身抗体。

    Methods To detect autoantibodies in 16 cases of sera from patients with pemphigus by immunofluorescence .

  18. 结果:通过反向mRNA斑点印迹技术可以从抑制消减杂交阳性克隆中把差异表达基因有效地筛选出来。

    Results : The differential expression genes are efficaciously screened from suppression subtractive hybridization positive clones using reverse mRNA dot blot technique .

  19. 蛋白印迹技术检测相关蛋白表明其作用与下调bcl-2有关,而与bax无明显关系。

    Western blot analysis suggested these were associated with down regulation of Bcl-2 , but not Bax .

  20. 采用SDS-PAGE电泳和蛋白免疫印迹技术鉴定表达及纯化产物。

    The products of expression and purification were identified by SDS-PAGE electrophoresis and Western blotting . 4 .

  21. 本文用免疫印迹技术检测122例风湿病患者的ENA多肽抗体。其中SLE阳性率为74.0%(37/50);

    We studied ENA polypeptide antibodies of 122 rheumatisants by technique of immunoblotting .

  22. 通过免疫印迹技术和细胞免疫化学测定tau蛋白的磷酸化程度及相关酶的活性。

    Tau hyperphosphorylation and the related enzymes were assayed by Western blot and cell immunochemistry .

  23. Southern印迹技术检测人9种恶性淋巴瘤细胞系IgCλ基因的重排

    Detection of Immunoglobulin C_ λ Gene Rearrangement in Nine Kinds of Malignant Lymphoma Cell Lines Using Southern Blotting Technique

  24. 应用免疫细胞化学及蛋白质斑点印迹技术检测NSE的免疫反应性(IR)。

    NSE immunoreactivity ( IR ) was detected by immunocytochemistry and protein dot bolt . The NO was detected by nitrate reductase method .

  25. 所以,通过分子印迹技术制备TiO2纳米粒子,以期提高TiO2选择性吸附和光催化降解目标污染物的能力。

    Therefore , the TiO2 was prepared by molecular imprinting technique in order to enhance its selective adsorption and photocatalytic ability towards the target contaminants .

  26. 分子印迹技术(molecularimprintingtechnology,MIT)是在仿生科学和模拟自然界中酶-底物及受体-抗体作用的基础之上发展起来的一项技术。

    Molecular imprinting technique ( MIT ) is a technology developed on the basis of the bionic science , as well as the simulation of enzyme-substrate and antigen-antibody interactions in the nature .

  27. 免疫印迹技术检测抗ENA抗体谱的应用现状及技术进展

    Applied Status and Technical Improvement of Anti-ENA Autoantibody Profile Detection by Immunoblotting Technique

  28. 通过免疫荧光和免疫印迹技术比较了LPP与类天疱疮(BP)抗原在一些免疫特性方面的区别。

    Immunologic characteristics of LPP antigen in this patient were investigated by indirect immunofluorescence immunoblotting and compared with bullous pemphigoid ( BP ) antigen .

  29. 免疫印迹技术研究FAK表达的变化。

    Western blot technique was used to study the expression of FAK at protein level with ECL reagents .

  30. 方法:体外培养HUVEC系用逆转录聚合酶链反应(RT-PCR)和Western印迹技术,检测HUVEC细胞中的LnkmRNA及蛋白表达。

    Methods : Reversal transcription polymerase reaction and Western blot were respectively performed to detect the expression of Lnk mRNA and protein in cultured HUVEC in vitro .