原噬菌体

初步明确了中国存在的原噬菌体类型及其在不同地区的分布情况。

Prophage types and corresponding distribution had been defined preliminary .

该研究证实了编码Stx的原噬菌体具有不稳定性，原噬菌体对O157的致病性具有重要作用，也提示原噬菌体在致病菌的进化过程中也具有重要作用。

The results of this study confirm the instability of prophage , and suggested that the prophage plays important role in the pathogenicity of O157 ∶ H7 , and in the evolution of pathogenic bacteria .

说明在λ原噬菌体紫外诱导过程中产生了自由基，而且SOD能有效的消除这些自由基。

It indicted that free radicals were generated in the process of UV-induced of λ . lysogenic bacteria , and that SOD could efficiently quench the free radicals .

应用微核试验、Ames试验和原噬菌体诱导试验检测富锌酵母的致突变性。试验结果显示，富锌酵母浓度在2.5g／kg、1.3g／kg、0.6g／kg时，微核试验阴性。

This paper reported the results of mutagenesis of Zinc-rich yeast assayed with the Ames test , micronucleus test and phage induce test .

HX与XO作用一定的时间后，再对λ原噬菌体进行诱导，其诱导效果随反应时间的延长而下降。

On the contrary , the titers of the bacteriophage decreased quickly with the elongation of the action time of HX and XO .

用SOS反应原噬菌体诱导法筛检14种常用抗肿瘤药物的致突变性。

The 14 kinds of common antineoplastic drugs were tested for their mutational by using the SOS induct test whether the S9 was added to or not .

方法：回顾性的分析本科近6年收集的71例化妆品皮炎的临床资料。实验室检查采用斑贴试验和原噬菌体诱导法（SOS试验）。

Methods : 71 patients with cosmetic dermatitis over 6 years were retrospectively studied , and laboratory examinations by patch test and bacteriophage induction method ( SOS test ) were performed at the same time .

本研究利用聚合酶链式反应技术，成功地克隆了枯草芽孢杆菌缺陷型原噬菌体PBSX阻遏基因及其温度敏感型等位基因。

The repressor gene and its allele-a temperature sensitive mutant have been cloned from the defective prophage PBSX of Bacillus subtilis by means of PCR technique .

0157：H7另外一个毒力因子是由插入到染色体上的原噬菌体编码的志贺毒素（Stx），它能使感染的病人器官发生病理变化，或者继发其它的临床症状而引起死亡。

Another important virulence factor of EHEC O157 : H7 was Shiga-toxin ( Stx ), which was encoded by a bacteriophage inserted into the chromosome of O157 : H7 . This potent cytotoxin is the factor that leads to death and many other symptoms in patients infected with EHEC .

λ原噬菌体缺失突变株的诱导和分离

Induction and Isolation of the Mutants Deleted in λ Prophage DNA

测定λ原噬菌体诱导频率的新方法

New Methods for Determining Induction Rate of λ Prophage in Lysogenic Bacteria

本研究发现HX&XO体系如同紫外辐射一样，能诱导λ原噬菌体从溶原状态进入裂解生长。

It was found that HX - XO system could induce lambda prophage from lysogenic state to lytic growth as well as UV irradiation .

应用原噬菌体诱导法检测35种不同化学物质的潜在遗传毒性，S9作代谢激活系统。

The possible mutagenic activity of 35 different chemicals has been tested with inductest , in which 89 mixture was used as a metabolizing system .

这种重组质粒将在进一步研究λ原噬菌体的诱导机理以及受紫外辐射损伤细胞的修复作用等方面成为有效的工具。

This recombinant plasmid will be a useful tool in the studies of the inductive mechanism of λ prophage and the repair of cells damaged by UV-irradiation .

本文报道两种测定λ原噬菌体紫外诱导频率的新方法&菌落计数法和平板诱导法。

Two new methods , colony counting method and plate inducing method , were established for determining ultraviolet induction rate of λ prophage in the present study .

以上结果说明，自由基在入原噬菌体紫外诱导中起着重要的作用，内源和外源的谷胱甘肽均可以有效地清除诱导过程中产生的自由基，从而抑制λ原噬菌体的紫外诱导。

These results indicate that GSH can significantly inhibit the UV induction of lambda prophage , and that this effect is correlated to its capacity to scavenge free radicals generated in the process of UV irradiation .

序列比对及扩增电泳类型分布分析表明，云南、四川等高海拔地区种群与其他低海拔地区种群相比，株系更为复杂，且原噬菌体活性更为丰富。

Sequence alignment and the distribution of E-type indicated that the prophage was more active and strains were also more complex in high altitude regions , like Yunnan and Sichuan , than that in low altitude regions .

用PCR法检测苏云金杆菌的溶原性噬菌体

Detection of Lysogenic Phage from Bacillus thuringiensis by PCR Method

方法：用12株鼠伤寒沙门菌分型噬菌体对3株体外诱导的鼠伤寒沙门菌L型及其原菌做噬菌体敏感性检测。

Methods : Phage sensitivity of 3 strains of Salmonella typhimurium Lforms induced in vitro and their parents were tested by 12 strains of typing bacteriophages of Salmonella typhimurium .

溶原现象：噬菌体侵染了某些细菌后，在噬菌体内发生的一种生命周期。

Lysogeny : Type of life cycle that takes place in a Bacteriophage after it infects certain types of Bacteria .

产生葡激酶的溶原性转换噬菌体的研究&Ⅱ.由双溶原性菌分离的转换和不能转换噬菌体的比较

Studies on the lysogenic conversion of Staphylococcus aureus for the production of staphylokinase ⅱ . comparison of the converting and non-converting phages isolated from a double lysogenic strain of S.aureus

本研究详细探讨了谷胱甘肽基因在溶原菌中的表达及对λ原噬菌体紫外诱导的影响。

The effect of GSH on the ultraviolet ( UV ) induction of lambda prophage was investigated in lysogenic Escherichia coli in present study .

结果表明recA基因在溶原菌中表现出明显的生理功能，能使λ原噬菌体从溶原状态进入裂解循环。

The results indicated that the recA gene showed a remarkable physiological function in the λ lysogenic bacteria . It could induce the λ prophage to enter the lysis cycle from lysogenic state .