原生质

用毒素粗提液处理寄主愈伤组织2d后，可于光学显微镜下观察到严重的细胞原生质泡囊化现象，这是寄主受到毒素作用后于细胞水平所表现出的伤害症状。

After the host callus cells were inoculated with the toxin , the many bubbles in cell plasma were observed in 2 days .

按顶体的形态可以划分为十种类型：（1）原生质膜及顶体完好。

The change of acrosomal morphology may be classified into 10 categories : ( 1 ) plasma membrane and acrosome intact .

质粒DNA浓度为20μg/2×10~6原生质体。

The concentration of plasmid was 20ug / 2 × 106 protoplasts .

玉米黑粉菌DNA载体的构建和它的原生质球的转化和再生

The construction of DNA vector and spheroplast transformation and regeneration of Ustilago maydis

本文采用原生质体裂解法制备高分子量平菇总DNA。

Pleurotus DNA was prepared by protoplast lysis .

原生质体融合菌株F6酿造蜂蜜桑椹酒的发酵条件优化

Optimization of the Fermentation Conditions of Honey-mulberry Wine with Fusant F_6

赤霉菌原生质体DNA转化

DNA transformation of Gibberella fujikuroi protoplast

低温锻炼期间柑桔原生质体SOD和CAT酶活性的变化

Changes of SOD and CAT Activities of Citrus Protoplast During Cold Acclimation

He-Ne激光诱变原生质体选育四环素高产菌的研究

Laser mutation on Protoplasts and the breeding of tetracycline high-yielding strain

获得了粳型水稻77-170品系原生质体再生植株（R2）6个株系的206棵后代植株。

A total number of 206 regenerated plants ( R2 ) of 6 plant lines of rice strain 77-170 from protoplasts were obtained .

同时，对影响PEG转化的渚多因素，如PEG的毒性、转化后的原生质体稳定性等进行了讨论。

Some factors such as PEG toxicity and protoplast stability affecting PEG-mediated transformation were discussed .

黄背木耳HD2原生质体再生菌株子实体农艺性状分析与研究

Study on the agricultural character of protoplast regeneration strains of Auricularia polytricha HD_2

原生质体形成的愈伤组织在UM培养基上分化体细胞胚。

Proto-plast-derived calli developed somatic embryos on UM medium .

电穿孔法诱导GUS基因在坛紫菜原生质体中的瞬间表达

Transient expression of GUS gene in Porphyra haitanensis by electroporation transformation

软枣猕猴桃原生质体培养及GFP基因转化研究

Study on the Protoplast Culture and GFP Gene Transformation of Actinidia Arguta

建立了烟草原生质体稳定培养体系，在含GFP基因的质粒单独转化原生质体的处理中，统计结果显示转化率最低，在0.3%左右；

The stable cultivate system of tobacco protoplast established .

啤酒酵母（saccharomycescerevisiae）营养缺陷型的筛选及原生质体融合

Auxotrophic Mutant Selection and Protoplast Fusion in Saccharomyces cerevisiae

在B5原生质体培养基中，A（11）B2的原生质体再生细胞形成小细胞团；

When protoplasts isolated from callus line A_ ( 11 ) B_2 were cultured on the modified B_ (?) medium , cell division was arrested at the stage of cell clusters .

合子原生质体CTC荧光呈极性分布。

The CTC fluorescence was polar distributed in the zygote protoplast .

原生质体融合选育高效菌株净化PVA工业废水的研究

Study on Breeding of high efficient strain with the technique of protoplast fusion for cleaning industrial wastewater containing PVA

明确了BSA（牛血清白蛋白）在稳定原生质体活力方面的作用。

BSA ( Bovine Serum Albumin ) also had some effect on constant isolation of protoplasts .

He-Ne激光诱变黑曲霉Sx的原生质体

He Ne laser treatment of the protoplast from Aspergillus niger SX

还探讨了在原生质体培养10天后加入提高了pH值的培养液来控制细胞褐化的可能性。

The possibility to control cell browning by adding higher pH value protoplast culture medium to the culture which had cultured the protoplast for 10 days was discussed .

制备该菌原生质体的最佳菌龄为从孢子接种起培养24h。

The optimum mycelial age for preparation of protoplast was at 24h time-point after inoculation and culture of conidia .

糙皮侧耳（Pleurotusostreatus）种内原生质体融合与基因定位

Protoplast fusion and gene location in Pleurotus ostreatus

研究了软X射线对丹西红桔（Citrusreticulata）愈伤组织和原生质体的影响。

Experiments were performed to investigate the effects of X ray on callus and protoplasts of Dancy tangerine ( Citrus reticulata ) .

确定了NND52C菌株原生质体制备和再生的条件，其原生质体形成率达到108个mL，再生率约为02%，转化率为102～103个转化子μg质粒DNA。

As a result , protoplast formation rate and regeneration rate reached 108 / mL and 0.2 % , respectively . Transformation rate was 102 ~ 103 / μ g plasmid DNA .

影响菜用芥菜原生质体培养和植株再生的因素及PAL活性与分化的关系

Factors Influencing Culture of Leaf Protoplasts of Vegetable Mustard and Plant Regeneration , and Relationship between Activity of PAL and Differentiation

龟裂链霉菌（Streptomycesrimosus）原生质体制备与再生条件研究

Studies on Protoplast Preparation and Regeneration Conditions of Streptomyces rimosus

探讨了菌丝培养条件对食用担子菌香菇（Lentinusedodes）原生质体释放的影响。

Effects of mycelium incubation conditions on the release of protoplasts from edible fungus Lentinus edodes were examined .