合成多肽

用丙型肝炎病毒（HCV）核心区2个型特异性合成多肽作为包被抗原，建立了ELISA法，对血清中抗－HCV进行分型研究。

We used two synthetic peptides deduced from the putative core protein of hepatitis C virus ( HCV ) to set up the ELISA systems for differentiating the HCV antibodies into two types .

目的比较多发性硬化（MS）患者和正常人T淋巴细胞对碱性髓鞘蛋白（MBP）和蛋白脂质蛋白（PLP）及其合成多肽的增殖反应性。

Objective To compare the proliferative responses of T cell line ( TCL ) to myelin basic protein ( MBP ), proteolipid protein ( PLP ) and their synthetic peptides in multiple sclerosis ( MS ) patient .

抗溶葡萄球菌酶N端合成多肽抗体的制备与鉴定

Preparation and Identification of Anti-lysostaphin N terminal Antigenic Peptide Antibody

合成多肽抗原检测原发性肝癌血清标志物ELISA方法的建立及应用

Detection of Serum Markers of Primary Hepatocellular Carcinoma by ELISA Using Synthetic Polypeptide Antigen

结论利用合成多肽TAP成功制备了抗TAP单克隆抗体。

Conclusion The mAb against TAP is prepared successfully .

以HEV合成多肽为抗原制备抗－HEV免疫血清

Preparation of anti - HEV serum using the antigens of synthetic peptides

将鉴定的表位合成多肽，混合包被ELISA板，证实可以用于检测猪血清。

The identified epitopes were synthesized as polypeptides . Elisa plates were coated with mixing synthesized polypeptide to detect porcine sera .

HPLC分离MALDI-TOF-MS鉴定合成多肽的研究

Studies on the Separation and Characterization of Synthetic Peptide Using HPLC and MALDI-TOF-MS

用细胞内完整表达的蛋白代替合成多肽，可以不受HLA类型的限制，扩大了临床应用。

Expression of the complete protein instead of using its peptides broadens its clinical use without HLA typing limitation .

多肽研究XX：丙型肝炎病毒（HCV）合成多肽的抗原性及线性抗体谱

Studies on synthetic peptide xx : the antigenicity and linear epitope map of synthetic peptide hepatitis C virus

合成多肽为半抗原制备抗SCF受体单克隆抗体

Generation of mAbs against synthetic SCF receptor peptides

以合成多肽为抗原检测EB病毒抗体的酶免疫测定法

The development of enzyme-linked immunosorbent assay for the detection of anti EBV antibody using synthetic peptide antigen

介绍固相合成多肽中的HBr气流装置

Introduce HBr Airflow Produce in Solid Phase Polypeptide Synthesis

抗Resistin合成多肽抗体的制备及鉴定

Preparation of anti-resistin peptide antibody

目的研究SDS-PAGE分析小分子合成多肽胸腺素α1的方法。

Objective To investigate a sensitive method of electrophoresis for analysis of low molecular weight peptides , such as thymosin α _1.Methods Tricine-SDS-PAGE was used .

方法底物制备采用N-羟基琥珀酰亚胺活化酯合成多肽法；建立分光光度法测定ACE的活性。

Methods The substrate was prepared by the use of N-hydroxysuccinimide ester in peptide synthesis , and serum ACE was determined by spectrophotometric assay using substrate .

阳性噬菌体克隆呈现多肽和人工合成多肽对IL5介导的嗜酸细胞（Eos）存活的影响。

The displayed polypeptides of positive phage clones with high affinity and synthesized polypeptides were tested for their effects on IL-5-mediated eosinophils survival .

通过合成多肽制备的抗体有特异性，WESTERNBLOTTING检测可见U-box家族的蛋白质表达谱具有两种模式，一种为组成型表达，另一种为组织特异性表达。

Western blotting detection of U-box protein family has two profiles , one for the constitutive expression , and the other for the tissue-specific expression .

结论：采用人工合成多肽作为半抗原成功地制备出抗β-Netrin的pAb和mAb。

CONCLUSION : Using the synthesized peptides as hapten , we have prepared epitope-specific mAbs and pAb against β - Netrin successfully .

目的：探讨抗人工合成多肽自身抗体在扩张型心肌病（DCM）诊断中的价值。

Objective : To investigate the diagnostic value of autoantibodies against cardiac peptides in dilated cardiomyopathy ( DCM ) by using antigens of synthetic peptides in the examination .

结果合成多肽N2（第12～22位）、C3（第265～275位）可与C5a发生特异性结合，结合率分别为53%和23%。

Results The synthesized polypeptides ( N2 and C3 ) could bind to C5a specifically and their binding rate was 53 % and 23 % respectively .

Ugi反应作为多组分反应中最重要的反应之一，在合成多肽及其类似化合物和杂环化合物领域中有着广泛的应用。

Multicomponent Ugi reaction is one of the most important reactions in the synthesis of similar compounds and heterocyclic compounds .

以丙型肝炎病毒（HCV）结构区和非结构区基因工程表达抗原及人工合成多肽包被酶标板，用辣根过氧化物酶标记抗原，建立了检测血清中抗－HCV总抗体的双抗原夹心法。

An antigen sandwiched ELISA for detection of the total antibodies to HCV was established using genetically engineered proteins and synthesized peptides located in the structural and nonstructural regions of HCV genomes as coated antigens and were labeled by horseradish peroxidase , respectively .

FBL-3肿瘤抗原的实验研究：Ⅰ.抗原的预测及合成多肽抗原性的验证

Study on the Antigens of Tumor FBL-3 : I.Prediction of FBL-3 Antigen and Verification of Antigenicity of Synthetic Peptides

固相法合成多肽AN-M的工艺研究合成多肽的电喷雾质谱研究

Study on Artwork of Fmoc Solid-Phase Synthesis of AN-M Identification and Analysis of Polypeptides by Electrospray Ionization Tandem Mass Spectrometry

方法采用HEVORF2、ORF3合成多肽单独和联合酶联免疫法，动态检测52例病人血清抗HEV。

Methods Anti HEV was detected in serial sera of 52 patients , by an enzyme linked immunosorbent assay ( EIA ) based on two synthetic peptides separated or combined from open reading frame ( ORF ) 2 and 3 of HEV genome .

用计算机软件预测抗原决定簇来设计多肽，通过合成多肽免疫动物制备抗体，检测水稻在不同发育时期的表达谱，对U-box蛋白质的表达水平进行分析。

Epitope prediction were carried out by computer software to synthesize polypeptides . Polyclonal antibodies were generated by rabbit immunization . Western blotting analysis were carried out for rice material collected at different developmental stages to detect expression pattern .

用非放射的31P-NMR新方法研究具底物1（IRS1）基序的合成多肽的体外磷酸化，可推测磷酸化的反应动力学机理。

A new protocol by nonradioactive 31P-NMR spectroscopy has been used to investigate in vitro phosphorylation in synthetic peptides with the motif of insulin receptor substrate 1 ( IRS1 ) . A dynamic mechanism of phosphorylation could be inferred from this investigation .

一种合成多肽&PLNG自由穿膜现象初探

Cell membrane penetrating property of a novel synthesized peptides & PLNG

固载化酶催化合成多肽的研究进展

Progress in the Study on Peptide Synthesis Catalyzed by Immobilized Enzyme