基因转染技术

目的观察光化学基因转染技术对阳离子聚合物介导目的DNA转染结肠癌细胞效率的影响。

Objective To investigate the effect of photochemical gene transfection on colon cancer cells mediated by polycation .

利用基因转染技术长期培养人甲状腺细胞

Long term culture of human thyroid epithelial cells with gene transfection

应用脂质体基因转染技术建立稳定表达CyclinG2基因的胃癌克隆细胞，为深入研究CyclinG2在胃癌细胞周期调控的作用和机制提供理想的生物学模型。

In order to build up a gastric carcinoma cell expressing human cyclin G2 gene stably , a useful tool for the research on the function of cyclin G2 in gastric carcinoma cell cycle regulation was made .

一种新的离体培养大鼠海马神经元基因转染技术

A New Method of Gene Transfection into Cultured Hippocampal Neurons of Rats

体内心肌基因转染技术的研究进展

Progression in myocardium gene transfection technique in vivo

光化学基因转染技术及诱导结肠癌细胞分化的实验研究

The Study of Photochemical Gene Transfection and Differentiation Induction on Human Colon Carcinoma Cells

本文仅就目前体内心肌基因转染技术的进展作一综述。

This article reviews about progress in gene transfection technique to myocardium in vivo .

基因转染技术提高体内构建组织工程化软骨质量的实验研究

Experimental study on applying TGF - β 1 gene transfection to modify in vivo tissue engineered cartilage

骨髓间充质干细胞与组织工程支架的联合应用，与基因转染技术的联合应用方面还有广阔的研究空间，待广大学者研究探讨。

There are many prospective aspects for the transplantation of MSCs combined with tissue engineering scaffold and gene transfection technique .

方法通过基因转染技术，分析白介素-22蛋白对恶性黑色素瘤细胞生长的影响。

Methods Using IL-22 supernatant as medium pour in A375 cell , after gene transfection , cell growth was measured by Alamarblue analysis .

利用基因转染技术将野生型p53cDNA转入肿瘤细胞可引起肿瘤细胞凋亡和生长抑制。

Using gene transfection techniques to transfer the wild type p53 cDNA into tumor cells can induce tumor cells apoptosis and inhibition of progression .

利用基因转染技术结合组织工程有助于深入加强骨缺损的修复研究。

The approach combining the principles of tissue engineering with technique of gene transfection , could be of potential benefit to accelerate bone defect repair .

基因转染技术是将外源基因转染真核细胞的一种技术，本文将对外源基因转染真核细胞的目的意义、转染技术分类、方法及其应用等做一综述。

Gene transfection is a technology that heterologous genes transfect eukaryotic cells , this article will summarize the significance , classification , methods and application of transfection .

新技术和新方法（尤其是干细胞技术和基因转染技术）的应用，将为皮肤组织工程技术注入新的活力和希望。

In the near future , new techniques and methods will provide more energy and hopes to the tissue engineering of skin , especially the application of the stem cell and gene transfection .

应用基因转染技术，诱导肿瘤细胞表达某种表面抗原或受体，使肿瘤细胞能够选择性地摄取某一放射性核素或其标记物，以便能够应用放射性核素治疗恶性肿瘤。

This review presents an overview of gene transfection methods to genetically induce tumor cells to express enhanced levels of cell surface antigens and receptors to intake radiolabeled antibody and peptide targeting and thus increase their therapeutic effect in radiotherapy .

方法：通过基因稳定转染技术，构建ERα稳定表达的MDA-MB-435细胞株。

Methods Human ER α cDNA was reintroduced into MDA-MB-435 cells by stable transfection .

结论Tf能增强脂质体-基因转染，介入技术与Tf联合双重靶向对肝癌的基因治疗具有相当的应用前景。

Conclusion Tf can enhance gene - liposome transfection efficiency , furthermore with combination of interventional catheter technique , there would be a potential duel-target-orientated gene therapy method . ( J Intervent Radiol , 2007,16:99-103 )

利用基因转染或RNA干扰技术进行体外或体内功能研究。

Functions of significant molecules were studied by gene transfection or RNAi .

方法：将B7基因采用脂质体转染技术，将PLXSN/B71转染肾癌细胞。

Methods : PLXSN / B7 was transfected into renal carcinoma cells through liposome , the expression of B7 1 was observed by RT PCR .

基因克隆和基因转染技术的发展让内耳基因治疗前景广阔。

Developments of gene cloning and gene transfection have broaded outlook of NT-3 gene therapy .

目的：采用原代培养技术进行大鼠原代细胞的培养与增殖，并模拟基因转染中的电穿刺激，探讨大鼠成肌细胞在基因转染技术中可能的应用前景。

AIM To investigate the possible application prospect of myoblasts of rats in the gene transfection following the culture and proliferation of primary cells of the rats were performed with primary culture method and the simulation of electroporation in the gene transfection was simulated .

结论静脉注射黏附质粒的微泡声学造影剂同时经体表给予一定强度的超声辐照，能显著增强辐照部位局部组织的基因转染与表达，有望成为一种安全、高效的体内基因定位转染新技术。

Conclusion Local ultrasound irradiation after intravenous injection of plasmid adhered on the microbubble could highly enhance the local gene transfection and expression . This method may provide a safe , high effective local gene delivery technique in vivo .