扩增片段长度多态性
- amplified fragment length polymorphism
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目的采用扩增片段长度多态性DNA(AFLP)分子遗传标志技术,分析人参、西洋参基因组DNA多态性。
AIM To analyse the genetic polymorphisms of Panax ginseng and P.quinquefolium by amplified fragment length polymorphism DNA ( AFLP ) molecular genetic marker technique .
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扩增片段长度多态性(AFLP)被称为“下一代分子标记”,是检测DNA多态性的一种新技术。
Amplified Fragment Length Polymorphism ( AFLP ) is called a " descendant molecular marker ", and it is a new technique in detecting DNA polymorphism .
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A族链球菌的随机扩增片段长度多态性DNA分型
Typing of group A streptococci by randomly amplified polymorphic DNA assay
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方法以PCR技术,聚丙烯酰胺凝胶垂直电泳和银染法对小卫星区域D17S30位点的扩增片段长度多态性进行了研究。
Methods Using PCR , polyacryl gel vertical electrophoresis and silver staining .
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通过琼脂糖电泳,分析不同核背景下ZFLl基因片段PCR扩增片段长度多态性。
We analyzed objective fragment length polymorphisms with different genetic background by agarose electrophoresis .
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方法应用PCR扩增片段长度多态性分析方法对100名无血缘关系的朝鲜族个体进行了调查。结果D16S539基因座,观察到6个等位基因,18种基因型;
Methods One hundred unrelated individuals were analyzed by PCR amplification fragment length polymorphism analysis .
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白介素4基因启动子(C590T)通过PCR限制性扩增片段长度多态性(PCRRFLP)方法检测。
IL-4 C-590T polymorphisms were determined by PCR-RFLP ( restriction fragments length polymorphisms ) .
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以PCR技术为基础的差异显示PCR、cDNA扩增片段长度多态性;
Differential Display Reverse Transcription-PCR ( DDRT-PCR ), cDNA Amplified Fragment Length Polymorphism ( cDNA-AFLP ) based on PCR technology ;
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目的:获得能用于中药天麻扩增片段长度多态性(AFLP)分析及其它分子生物学研究的高质量DNA。
Objective : TO extract high-quality DNA from Chinese traditional medicine Gastrodia elata for AFLP and other molecular biological analysis .
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扩增片段长度多态性(AFLP)在动物遗传育种中的应用进展
The Application Development of AFLP in Animal Genetic Breeding
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采用扩增片段长度多态性技术(AFLP)对奈瑟氏淋球菌菌株进行基因分型研究。
The amplified fragment length polymorphisms ( AFLP ) was applied in genotyping of Neisseria gonorrhoeae .
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方法对20个WD家系113名成员及100名正常对照组成员的DNA进行5个STR的聚合酶链反应(PCR)扩增片段长度多态性分析;
Methods The amplified fragment length polymorphism of the five STRs was analyzed in 20 Chinese WD families including 113 members and 100 normal controls .
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本文旨在通过cDNA扩增片段长度多态性(cDNA-AFLP)分离与肌内脂肪含量差异表达相关的基因。
Separation of differentially expressed genes related to IMF content by cDNA amplified fragment length polymorPHism ( cDNA-AFLP ) was conducted in this paper .
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AFLP(扩增片段长度多态性)是一种高效、快捷、稳定、可靠的DNA分子标记技术,在分子标记辅助育种上得到广泛应用。
AFLP ( amplified fragment length polymorphism ) is a DNA molecular marker technique with high efficiency , promptitude , stability and accuracy . It has been widely used in molecular marker-assisted breeding .
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vWFⅢ基因座扩增片段长度多态性及其法医学应用
Amplified fragment length polymorphism of a short tandem repeat locus ( vWF ⅲ) in a Chinese Han population and its application to forensic science practice
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目的研究山东产丹参的遗传多样性,初步探讨扩增片段长度多态性(AFLP)分子标记技术在丹参道地性鉴别上的应用。
ObjectiveTo study the genetic diversity of Salvia miltiorrhiza in Shandong and the application of amplified fragment length polymorphism ( AFLP ) to the identification of natural herb .
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方法:使用扩增片段长度多态性(Amp-Flp)和PCR结合聚丙烯酰胺凝胶电脉及银染技术,对300名广西地区壮族无关个体D1S80位点多态性分析。
Methods : 300 unrelated individuals in Zhuang were analyzed by PCR and PAGE following silver staining and amplified fragment length polymorphism .
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运用扩增片段长度多态性(AFLP)方法,研究了来自江西5个不同产地栀子的亲缘关系,并运用HPLC方法测定了栀子苷的含量,用ICP法测定了植物药材中的矿质元素含量。
By using amplified fragment length polymorphism ( AFLP ) markers , this paper studied the genetic relationships among five wild or cultivated Gardenia jasminoides Ellis populations in Jiangxi Province . Chemical fingerprint was also built with HPLC method .
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采用苯丙氨酸羟化酶基因内短串联重复序列扩增片段长度多态性连锁分析法,对22个苯丙酮尿症(PKU)家系进行了分析。
The short tandem repeats ( STR ) in intron 3 of phenylalanine hydroxylase gene were analyzed in 22 phenylketonuria ( PKU ) families by linkage analysis of amplified-fragment length polymorphism .
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本研究构建了大菱鲆扩增片段长度多态性(AFLP)分析体系,对DNA提取、双酶切反应、连接反应、预扩增反应、选择性扩增反应和银染等步骤进行了分析。
AFLP ( Amplified Fragment Length Polymorphism ) analysis system for turbot was established in this study , with the relative processes being presented , including DNA extraction , double enzymes digestion reaction , adapter ligation reaction , pre-amplification and selective amplification reactions , and argent dyeing .
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扩增片段长度多态性技术对淋球菌的基因分型
Molecule typing of neisseria gonorrhoeae by amplified fragment length polymorphism
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大连市伤寒沙门菌扩增片段长度多态性分型
Genotyping of Salmonella typhi sampled in Dalian area by amplified fragment length polymorphism
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四川地区鸡场产气荚膜梭菌的扩增片段长度多态性分析
Analysis of chicken Clostridium perfringens isolated from Sichuan province by amplified fragment length polymorphism
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扩增片段长度多态性实验技术及在动物遗传多样性研究中的应用
Amplified fragment length polymorphism ( AFLP ) and its applications in genetic diversity research of animal
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基因分析,抽提家系成员的DNA,进行PCR扩增,扩增片段长度多态性的检测采用银染色法。
DNA from the family members was assayed by PCR and the amplified fragment length polymorphism by silver staining method .
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本文着重介绍了随机扩增多态性DNA、聚合酶链式反应-单链构象多态性、扩增片段长度多态性3种重要的DNA指纹技术在污染土壤诊断中的应用。
In this paper , the application of three main DNA fingerprints in eco-toxicological diagnosis of contaminated soil was discussed emphatically .