新霉素抗性基因

各组髓核组织内新霉素抗性基因表达水平比较：绿色荧光蛋白细胞移植组及BMP-绿色荧光蛋白细胞移植组术后1,3,6个月组间及组内比较，差异均无显著性意义（P>0.05）。

Comparison of the level of neomycin resistance gene in the nucleus pulposus tissues of each group : There was no significant difference between the GFP transplantation group and BMP-GFP cell transplantation group at months 1 , 3 and 6 ( P > 0.05 ) .

DNA-PCR分析新霉素抗性基因的DNA拷贝数。

DNA-PCR was used to analyze the copies of DNA of neomycin-resistant gene .

结果建立分别稳定表达bcl-2基因、新霉素抗性基因（neo）的膀胱癌亚克隆细胞株BIU87/bcl-2、BIU87/neo。

Results The subclone bladder carcinoma cell lines , stably expressing bcl-2 and neo gene respectively , were successfully selected , named as BIU87 / bcl-2 and BIU87 / neo .

利用携带新霉素抗性基因（NeoR）的假型逆转录病毒载体（VSV-G/MuLV）转导培养的乳鼠心肌成纤维细胞，用G418筛选法观察基因转导效果。

Immunocytochemical staining was used to evaluate cardiac fibroblasts ; Neo ~ r gene was transduced into cardiac fibroblasts using pseudotyped MuLV ( VSV-G / MuLV ) vector ; Its effect was observed by means of G418 selection .

潮霉素和新霉素抗性基因转基因小鼠的建立及其表达研究

Generation of Transgenic Mice for Hygromycin and Neomycin Resistance Genes and Studies on Transgene Expression