柱一

表达产物经Ni柱一步分离后得到纯化。

The product was purified one step by Ni NTA column .

在选定的色谱条件下，BLC粗酶液经IDA型Cu2＋－螯合膜色谱柱一步纯化，比活性平均提高4.7倍，回收率为67.7%。

The results showed that under the chromatographic conditions chosen , 67.7 % recovery of BLC attained with an overall 4.7 fold increase in specific activity in a single step .

用谷胱甘肽-sepharose4B亲和层析柱一步纯化法可简单、快速地得到高纯度的蛋白。

Using Glutathione-sepharose 4B affinity column can simply and rapidly purify the proteins .

对改进一柱一桩垂直度的探讨

Discussion on the improvement of vertical degree of one post for one pile technology

一柱一桩基础单层厂房的侧向变位问题

Lateral rigidity problem for a one story factory building which is one column with one pile

框架柱基为一柱一桩的基础形式往往难以保证柱子底部为理想刚接。

One column with one pile foundation can not ensure the bottom of frame to be rigid .

异形柱一框支短肢剪力墙结构在地震作用下非线性地震反应分析

Nonlinear Seismic Response Analysis of Frame Supported Short-Leg Shear Wall with Special Shaped Columns Structure Under Unusual Earthquakes

轴向压力对钻柱一阶固有频率随温度变化的影响程度远大于轴向拉力的影响程度。

The range of influence of axial pressure on the first-order inherent frequency is much higher than that of axial tension .

设计人员一般在一柱一桩基础上设置双向拉梁来约束桩顶水平位移，以保证基础的侧向稳定性，但转角位移却不能避免。

Designers know it is necessary to connect single pile cap with base beams to ensure the stability of cap , but the rotation of cap is not avoidable .

整党明年就要推进到基层，到各单位各企业，一柱一桩基础单层厂房的侧向变位问题

Next year Party consolidation will be extended to include units and enterprises at the grass-roots level . Lateral rigidity problem for a one story factory building which is one column with one pile

如果厂房的高度超过一层，那么，任何大跨度建筑物的下面几层就不容易或不可能天然采光。一柱一桩基础单层厂房的侧向变位问题

If it is more than one storey high , day lighting of the lower storeys in any large-span building becomes difficult or impossible . Lateral rigidity problem for a one story factory building which is one column with one pile

应用此亲和柱可一步纯化蛇毒中的NGF，大大简化了NGF纯化工艺。

This column simplifies the technology of NGF purification .

本文利用结构分析软件ANSYS，以某机场航站楼单层柱面网壳结构为例，追踪了柱面网壳+支承柱这一整体结构的荷载-位移全过程。

Based on FEM analysis software ANSYS , a cylindrical latticed dome of an aerodrome is analyzed as an example . The progress of load-displacement for the whole structure cylindrical latticed dome columns is record .

采用硫酸铵盐析、SephadexG-100凝胶过滤、DEAE-52纤维素柱层析一系列纯化步骤，得到电泳纯的亚硫酸盐还原酶，纯化倍数为115.30，酶回收率为18.36%。

The sulfite reductase was purified to electrophoretic homogeneity by ammonium sulfate salting-out , Sephadex G-100 gel filtration and DEAE-52 cellulose column chromatography . A 115.30-fold-purification was achieved and the recovery was 18.36 % .

银杏酸粗提物经硅胶柱色谱一次预净化处理后，即可在242nm或310nm波长处测定银杏酸。

The content of ginkgolic acids in crude extract can be determined directly by UV spectrophotometry at 242 nm or 310 nm after one-pre-purified-step by silica gel column chromatography .

充填浮选柱是一个复杂的气液固三相体系。

Packed flotation column is a compler gasliquid-solid three phase system .

直接反演非均匀介质柱的一种新方法

A New Direct Method for the Reconstruction of Inhomogeneous Dielectric Cylinders

缀条式轴心受压格构柱的一种简化计算方法

A Simplified Calculation of the Lace-Barred Lattice Column with Axial Pressure

开采主运巷保护煤柱的一次成功尝试

Making trial of mining the protective pillar at main conveying roadway

环形脉冲萃取柱是一种新的脉冲筛板萃取柱。

Annular pulsed - sieve-plate extraction column is a new extraction equipment .

九柱地滚球一种滚球游戏，用九根木柱做靶子。

A bowling game in which nine wooden pins are the target .

液相流动对柱晶一次间距的影响

Effect of liquid flow on primary arm spacings of constrained columnar crystals

用鸭柱与一小球合玩的滚木球游戏。

A bowling game played with such pins and a small ball .

精子通过葡聚糖凝胶柱就是一种过滤方法。

The column of sephadex was one of the methods for filtrating spermatozoa .

离子交换柱层析一步纯化法。

The second method was Ion Exchange Pole Chromatography .

将管柱置于一个干净的微量离心管。

Place the column in a clean microcentrifuge tube .

以宽分布标样标定凝胶渗透色谱柱的一种新方法

A New Method for Calibration of Gel Permeation Chromatographic Columns by Polydisperse Polymer Standards

可用定制的装饰护柱和一套静电喷塑罩面漆。

Custom decorative bollards and a range of powder coat finishes will be available .

钢筋混凝土柱的一种加固方法

A Way of Strengthening the Reinforced Concrete Pillars

方钢管组合异形柱是一种新型的异形柱形式。

Special-shaped combined column of square steel tubes is a new type of construction .