核原生质

香菇单核原生质体杂交菌株JW系列的选育研究

Study on the breeding of JW strains from protoplast monokaryon cross in Lentinus edodes

单核原生质体再生菌丝的菌落形态、菌丝生长速度和营养缺陷型标记等性状均稳定地保持了亲本的特性。

The characters of parents , such as morphology of colony , growth rate of mycelium and auxotroph , were maintained in protoplasted monokaryon .

以香菇菌株精选和武香作为亲本制备单核原生质体，将两个亲本的单核原生质体进行一一杂交。

Protoplasted monokapyon ( PM ) were prepared from Lentinus edodes strains Jingxuan and Wuxiang , respectively , intercross process were put in practice .

结果表明：双孢蘑菇同核原生质体的杂交是一个较复杂的性亲合与核迁移过程，其杂交率较低。

Results showed that cross between homokaryotic protoplast in Agaricus bisporus was a complicated procedure of sex compatibility and nuclear migration and its hybridization percentage was not high .

经测定，单核原生质体再生菌丝杂交后代菌丝生长速度和羧甲基纤维素酶活性的广义遗传力分别为61.0%和88.8%。

The broad-sense heritability of mycelium growth rate and cellulase activity in progeny of PM mating was 61 . 0 % and 88 . 8 % , respectively .

本文着重介绍了原生质体技术如单核和同核原生质体技术、诱变技术、原生质体融合技术、RAPD技术、RFLP技术等在食用菌遗传育种中的应用及研究进展概况。

The application of protoplast monokaryon technique , protoplast-induced mutation technique , protoplast fusion technique , RAPD technique and RFLP technique in genetic breeding of edible mushroom was reviewed in this paper .

每个细胞都具有核，细胞核位于原生质中。

Each cell possesses a nucleus imbedded in protoplasm .

本文阐述了食用菌单核和同核原生质体的发现和作用以及在食用菌遗传研究上的应用前景。

This paper elaborates the discovery and effect of monokaryon and homokaryon protoplasts and their application in genetic and breeding research of edible mushroom .