根癌

用根癌土壤杆菌S-1、702和C-58从向日葵胚轴、烟草茎和茎髓，胡萝卜根及马铃薯块茎诱发并建立了冠瘿株系。

Crown gall strains caused by Agrobacterium tumefaciens S-1702 , C-58 have been established from sunflower hypocotyls , tobacco stems and stem pithes , carrot roots and potato tubers .

核果类果树根癌病菌寄主范围及抗性研究初报

Primary Studies on the Host Range and its Resistance to Crown Gall of Stone Fruits

聚合酶链式反应结合Southern杂交检测外源DNA转化根癌农杆菌

Detection of the Transformation of Foreign DNA into Agrobacterium tumefaciens by PCR and Southern Biots

我国葡萄根癌农杆菌Ti质粒的特征

Characteristics of Agrobacterium tumefaciens Ti plasmid isolated from grapevine in China

根癌农杆菌介导的基因转化过程中，TDNA的整合是关系到外源基因能否稳定遗传的关键步骤。

The T-DNA integration is a critical step of the steady inheritance for foreign genes during the Agrobacterium tumefaciens-mediated transformation .

方法在根癌脓杆菌介导获得系列转基因马铃薯植株的基础上，用ELISA分析转基因马铃薯中目的蛋白表达水平。

Methods The expression of the foreign protein in the obtained transgenic potatoes was assayed by ELISA .

综述了根癌农杆菌介导真菌遗传转化的转化机理和TDNA在真菌细胞中的存在方式等方面的研究结果，并展望这一方法的应用前景。

The mechanism of transformation in fungi mediated by Agrobacterium tumefaciens and existence of T DNA in fungi cell were summarized in this review .

冻溶法将表达载体导入根癌农杆菌EHA105，PCR筛选阳性克隆，获得含有目的基因的工程菌。

The recombinant plasmid was transformed into Agrobacterium tumefaciens by freezing-thaw method and the engineered clones were further confirmed by PCR .

根癌农杆菌，根瘤土壤杆菌农杆菌Ri诱导丹参毛状根培养体系的优化

Agrobacterium tumefaciems Establishment of Hairy Roots Culture System of Salvia

根癌农杆菌介导的GFP在洋葱表皮细胞定位研究

Localization Analysis of GFP in Onion Epidermal Cell via Agrobacterium tumefaciens-Mediated Transformation

根癌农杆菌Ti质粒携带抗菌肽基因导入烟草

Transferring Antibacterial Peptide Gene from Chinese Oak Silkworm ( Antheraea pernyi ) into Tobacco Plant by Using Ti Plasmid of Agrobacterium tumefaciens

根癌农杆菌介导ACS反义基因转化文心兰的研究

Studies on the Transformation of Antisense ACS Gene Mediated by Agrobacterium Tumefaciens in Oncidium

根癌农杆菌介导转化甘蓝型油菜子叶GUS基因瞬间表达

Transient expression of GUS gene in cotyledons of rapeseed mediated by Agrobacterium tumefaciens

根癌农杆菌介导的球毛壳菌遗传转化及T-DNA插入突变体的获得

Agrobacterium tumefaciens-mediated transformation of Chaetomium globosum and its T-DNA insertional mutagenesis

根癌农杆菌介导甲型肝炎病毒（HAV）衣壳蛋白融合基因转化柑桔的研究

Agrobacterium-mediated Transformation of Citrus with Hepatitis A Virus Capsid Protein Fusion Gene

根癌农杆菌介导灰葡萄孢遗传转化及T-DNA侧翼序列分析

Agrobacterium-mediated Transformation of Botrytis Cinerea and Analysis of Flanking Sequences

根癌土壤杆菌浓度：5×108CFU／ml；

Density of Agrobacterium tumefaciens , 5 × 108 CFU / ml ;

本试验成功利用根癌农杆菌介导GFP转入稻瘟病菌，并进行了验证。

GFP was transformated successfully into rice blast fungus using Agrobacterium mediated method in this study , verified the transformation .

利用根癌农杆菌介导，以下胚轴为外植体把ACC解氨酶基因转入青花菜栽培品种上海2号，获得了转基因再生植株。

ACC deaminase gene was successfully introduced into broccoli ( Brassica oleracea var. Italica ) by Agrobacterium tumefaciens mediated transformation .

根癌农杆菌T-DNA在向日葵离体组织中的转移与表达

Transfer and expression of the T-DNA harboured by Agrobacterium tumefaciens in cultured explants of Helianthus annuus

以桃幼胚作为根癌农杆菌介导转化的受体，通过对GUS基因瞬时表达率的分析，研究此转化体系的最佳实验参数。

Using transient expression of GUS gene , the optimal conditions for Agrobacterium-mediated transformation of peach immature embryos was investigated .

香蕉根癌农杆菌介导ACS反义基因遗传转化及ACS基因克隆

Studies on the Transformation of Antisense ACS Gene Mediated by Agrobacterium Tumefaciens and Cloning of ACS Gene from the Leaves of Banana

吉林省大豆属对根癌农杆菌致瘤反应及T-DNA转移的研究

Study of Tumor Induced Reaction of Glycine Infected by Agrobacterium tumefaciens in Jilin Province and T-DNA Transfer

从月季品种金玛丽、曼海姆、杏花村、梅郎口红的根部肿瘤组织中纯化了9株分离物，根据其在MW选择性培养基上的单菌落形态初步判断其为根癌土壤杆菌。

Nine isolates were purified from the crown galls on rose plants and were characterized as Agrobacterium tumefaciens strains according to the colony morphology on the selective MW medium .

本研究利用Gus基因瞬时表达建立了根癌农杆菌介导的菊花遗传转化体系。

The optimal system for genetic transformation mediated by Agrobacterium tumefaciens was studied based on transient expression frequency of Gus gene .

根癌农杆菌介导gna基因对水稻的转化

Agrobacterium tumefaciens - mediated Transformation of Rice for gna Gene

AgNO3对根癌农杆菌介导的甘薯遗传转化的影响

Effect of AgNO_3 on Transformation of Sweetpotato By Agrobacterium tumefaciens

诱导根癌土壤杆菌vir区基因表达信号分子的构效研究

Structure-activity Research of Signal Factors Inducing Agrobacterium vir Genes

异源nifA~C对根癌土壤杆菌nif基因表达的调节作用

Role of the Heterologous nifA C Product in the Regulation of nif Expression in Agrobacterium tumefaciens

确定了共培养最佳方式是灰葡萄孢分生孢子和活化的根癌农杆菌共同涂于玻璃纸上培养，共培养的最佳时间为48h。

Conidia of B. cinerea and active Agrobacterium co-smeared on glass paper was the best way of culture . 48 h was the fittest co-cultivation time .