棘胸蛙

棘胸蛙线粒体基因组全序列的测序与分析

Complete Nucleotide Sequence and Gene Organization of the Mitochondrial Genome of Paa Spinosa

饲养雄性棘胸蛙生理常数及精巢的年周期变化

Seasonal changes in physiological constants and testes of captive male PAA spinosa

前期基于cytb较端片段序列研究表明，棘胸蛙遗传结构差异较大，暗示可能存在隐存物种。

Early genetic structure based on short fragment Cyt b of spinosa quite different , suggesting that there may be hidden to cryptic species .

因此本课题选取棘胸蛙作为研究对象，通过人工模拟细菌入侵及暖冬状态，研究其组织AA水平及其生物合成能力的变化规律。

So this article chose giant spiny frogs as the research objects to study the changing regularity of AA level in tissues and GLO activity by simulating bacteria invasion and warmer winter state .

通过与NCBI上已经发表的同源物种进行序列对比，确定测的基因序列是棘胸蛙MHCIIB基因序列，其与非洲爪蟾的相似性达到72%。

Comparison to homologous species which has been published on the NCBI show that the gene sequence obtained is MHC IIB gene sequences and72 % similarity with Xenopus .

与冷暴露前相比，两个冬眠温度下野生棘胸蛙肝脏和肾脏AA含量及GLO活力均有所下降，但仅12℃冬眠组的肝脏AA含量存在统计学差异（P0.05）。

Compared with pre-exposure , hepatic and renal AA concentrations as well as GLO activity of wild giant spiny frog decreased , but only hepatic AA level of frogs hibernating at 12 ℃ declined significantly ( P0.05 ) .

同时也证实了组织AA水平及GLO活力在一定程度上能够有效地反映棘胸蛙在细菌胁迫下的健康状况和生存力。

Moreover , to a certain extent , it also showed that AA levels in tissues and GLO activity could reflect frogs ' health condition and survivability effectively under the bacteria stress . 3 .

棘胸蛙繁殖期间输卵管变化的组织学研究

Histological Changes of Oviduct of Rana spinosa during Reproductive Season

几种激素对棘胸蛙人工催产作用研究

Studies on Artificially-induced Ovulation of Rana spinosa David by Several Kinds of Gonadotrophin

基于生物技术调控棘胸蛙繁殖与养殖的研究

Artificial Control on Reproduction and Breeding of Rana spinosa David based on Biotechnology

水温对棘胸蛙人工孵化的影响

Effects of Water Temperature for the Rana Spinosa Hatching

目前国内外关于棘胸蛙肝脏蛋白质组学研究未见报道。

Up to now the proteomic research of it has not been reported .

棘胸蛙（RanaspinosaDavid）的生态观察及人工饲养的初步调查

Elementary Investigation and Study to the Ecology and Raising of Rana spinosa David

在行为描述的基础上，对棘胸蛙的有关行为机制进行了探讨。

Based on the description of behavior , we also discussed the mechanism of behaviors .

赣东北棘胸蛙蝌蚪栖息地生物因子研究

Studies on biological factors of the habitat of Rana spinosa tadpoles in northeastern Jiangxi Province

此外，本研究还可以为棘胸蛙的养殖和保护提供一定的参考依据。

In addition , this study could offer reference for the breeding and protection of giant spiny frogs .

棘胸蛙是一种主要分布在我国南方各省山区的大型经济蛙类。

Rana spinosa is a kind of large economic frog which mainly lives in mountain districts of southern China .

结合以往的研究，广泛分布在中国南部的棘胸蛙可能至少包含2个隐种。

Combined with previous research , there are two cryptic species at least in p. spinosa in widely distributed in southern China .

此外在养殖条件下棘胸蛙的发病率较高，这进一步说明其对环境的敏感性。

In addition , there is high morbidity in giant spiny frogs ' breeding , which explains its sensitivity to the environment factors further .

但12℃对养殖个体无致死作用，这可能是由于长期暖驯化使养殖棘胸蛙对暖冬耐受力提高。

However , 12 ℃ has no lethal effect on cultured frogs , which probably because the acclimation enhanced their tolerance to warmer winter .

今后，进一步对2个单系接触带的分子生物学及其形态、行为学等研究将有利于阐明棘胸蛙隐种形成的机制。

In the future , further study of two monophyletic contact with molecular biology and morphology , behavior and so on , will be beneficial to clarify the mechanism of formation of cryptic species in p. spinosa .

说明注射热灭活的大肠杆菌可以刺激棘胸蛙脾巨噬细胞产生较多的氧自由基，增加机体的氧化程度。

However , all of the indexes recovered to the initial level within 24h . These results confirmed that injecting heat-killed E.coli could stimulate giant spiny frogs ' spleen macrophagocyte to produce more oxygen free radicals and increase the organismal oxidation degree .

鸣声研究是揭示棘胸蛙生物多样性的有效手段之一，但前提是需要全面了解该物种鸣声参数与其他因子（如环境因子、身体大小等）的相互关系。

The acoustic research is to reveal the the P. spinosa biological diversity means one , but only requires a comprehensive understanding of the species acoustic parameters and other factors ( such as the relationship between environmental factors , body size , etc. ) .

结果表明：棘胸蛙雄性鸣声特征测量参数与体重、体长无相关性，而鸣声特征中的鸣叫时长、音节时长与环境温度、水温、体温、泄殖腔温度存在负相关性。

The results showed that : male P. spinosa call characteristics measured were not correlate with body weight , body length , but the call duration , note duration were negative correlate with air temperature , water temperature , body temperature , cloacal temperature .