水稻基因组

Southern检测的结果进一步证实，外源基因已经整合到水稻基因组中。

Southern blotting analysis also indicated that ThEn-42 gene was inserted into rice genome .

结合先前定位的169个TDNA标签，对TDNA在水稻基因组中的整合特点进行了分析。

Integration patterns of T-DNA tags in rice genome were further analyzed using the 78 T-DNA tags and 169 ones reported previously .

分别经PCR和Southern点杂交分析，结果表明目的基因已整合到水稻基因组中。

PCR analysis and southern blot hybridization showed primarily that SP gene has been transferred into rice .

水稻基因组DNA的提取方法主要有SDS法和CTAB法。

Genomic DNA was extracted from rice mainly using SDS and CTAB method .

用PCR对6个水稻基因组BAC克隆进行了筛选，得到了水稻nmads1基因2933bp的序列。

Six rice genomic BAC clones were screened by PCR .

我们利用网上公布的水稻基因组的测序结果，经过数据处理和分析，构建了一个水稻亚种粳稻和籼稻间的DNA多态性数据库。

Through the downloading of the rice sequence and the data analysis , a DNA polymorphism database between japanica and indica was constructed .

基于水稻基因组序列SSR的多态性分析

Analysis of SSR Polymorphism by Genome-Scale Comparing Between Varieties in Rice

不同限制性内切酶消化和Southern杂交分析显示，这段重复DNA顺序以串联加散布的形式存在于水稻基因组中；

Different restriction enzymes digestion and Southern hybridization indicated that this sequence was organized in tandem array and dispersed in rice genome .

Southern杂交结果表明该片段以单拷贝形式存在于水稻基因组中。

The result of Southern blot showed that it was single-copy gene . The length of RB I was 680 bp .

T-DNA在水稻基因组DNA的插入，既有准确的插入，又有不准确的插入。

Both precise insertion and imprecise insertion existed when T-DNA integrated into rice genomic DNA .

利用水稻基因组序列数据开发SSR标记的方法

Developing SSR Markers Using Public Rice Genome Sequence Data

常规PCR和Southernblot结果表明T-DNA区已整合到水稻基因组中。

The results from normal PCR and Southern blot showed T-DNA has inserted into rice genome .

经过潮霉素抗性检测和PCR鉴定，证实目的基因已经整合到转基因水稻基因组中。

Both hygromycin resistance detection and PCR amplification confirmed that the target gene had been integrated into the genome of transgenic rice .

GUS染色和PCR分析证明Xa21基因已整合到水稻基因组中。

GUS staining and PCR analysis demonstrated that Xa 21 had been integrated into rice genome DNA .

适于TAIL-PCR模板的水稻基因组DNA提取方法的优化

Optimization of the Extraction Method of Rice Genomic DNA Suitable for TAIL-PCR Template

结果发现，在TDNA右边界和侧邻的水稻基因组序列的连接处，146%的TDNA标签含有3～74bp的填充序列。

At junctions of the right border and flanking rice sequences , 14.6 % of T-DNA tags ( 36 ) showed 3 ~ 74 bp of filler sequences .

拟南芥和水稻基因组中PLD家族分析

Genome-wide Analysis of PLD Family in Arabidopsis and Rice

基于株高性状的玉米EST序列与水稻基因组的比较研究

Comparative Analyses between EST Sequences Concerning Plant Height of Maize and Sequences of Rice Genome Database

对三系杂交稻T0代转基因植株进行PCR和Southern杂交检测（恢复系湘晴），表明外源基因已经整合进湘晴水稻基因组中。

The PCR and Southern analysis ( only in Xiangqing ) showed the anti-Waxy gene had been transferred into genome of these hybrid rice and expressed .

水稻基因组和EST序列的测定和分析以及水淹条件下乙醛酸循环相关基因功能的研究

Analyses of Rice Genome Sequences and Expressed Sequence Tags and Studies of Glyoxylate Cycle in Submerged Seedlings in Rice

T-DNA标签在转基因水稻基因组中的整合特点

Integration Patterns of T - DNA in Rice Genome

T-DNA标签在水稻基因组中的分布与特征

Distribution and Characterization of T-DNA Tags in Rice Genome

Southern杂交分析表明，LTP基因在水稻基因组中以基因家族的形式存在。

In addition , the result of Southern blot analysis revealed the existence of an LTP gene family in the rice genome .

计算识别水稻基因组中microRNA基因

Computational identification of novel family members of microRNA genes in Oryza sativa genome

应用平均分布于水稻基因组的21对SSR引物，对福建漳浦野生稻、海南野生稻共计62份材料的遗传多样性进行分析。

SSR primers were used to assess the genetic diversity of62 common wild rice individuals from Zhangpu County Fujian Province , Hainan Province .

本文利用水稻基因组序列主要研究了（1）水稻（Oryzasativa）基因组倍增；

In this paper , we studied ( 1 ) the rice ( Oryza sativa ) genome duplication ;

以水稻基因组DNA为模板，通过PCR扩增技术得到16kDa启动子片段，序列分析结果表明：获得的启动子片段的大小为931bp，与已报道的该启动子序列相比较，其核苷酸序列同源性为99.9%。

The sequence analysis showed that the obtained promoter fragment 931 nucleotides and shared homology of 99.9 % with the reported 16 kDa promoter .

潮霉素抗性植株的PCR检测和目的基因的PCR检测表明，阳性率达到90%以上，初步确定目的基因已经整合到水稻基因组中。

PCR analysis of Hygromycin resistance gene and target gene showed that the positive rate was100 % , and preliminarily determined that the target gene had been integrated into the rice genome . 3 .

基于对水稻基因组序列的注解和同源搜索的结果，用RTPCR结合测序的方法证明了水稻中至少有10个具有转录活性的trslike基因。

There are at least ten transcriptional trs-like genes in rice that have been confirmed by RT-PCR and sequencing , based on the annotation results of rice genome and homologous search .

采用分布于水稻基因组的221个SSR位点结合系谱分析法，研究了水稻籼粳亚种间杂交衍生系和亲本的遗传多样性和亲缘关系。

Studies on genetic diversity and genetic relatives of indica-japonica subspecies rice hybrid deriving pedigrees and the parents was conducted by using 221 SSR and pedigree analysis .