甲基绿
- 名methyl green
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方法:体外试验MTT法测存活率,甲基绿-派诺宁联合染色观察细胞凋亡形态,流式细胞术、琼脂糖凝胶电泳观察DNA损伤及细胞周期。
Methods : MTT , methyl green and pyronin stain assay , flow cytometry , agarose gel electrophoresis were used .
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小牛胸腺DNA溶液在100℃保温不同时间以后,测定了粘度的变化,同时观察并比较甲基绿与焦宁对于受热变性前后的DNA的结合能力的差异。
Saline solutions of calf thymus DNA have been heated at 100 ℃ for different periods and the change in viscosity followed in comparison with the changes in its affinity for two basic dyes , methyl green and pyronin .
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结果及结论:甲基绿-派洛宁-马休黄组合染色法能显示细胞质mRNA明显增高时期的凋亡神经元。
Results and Conclusion : The method of MG P MY staining can identify neuronal apoptosis only when mRNA is significantly increased in neuronal cytoplasm .
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Fe-H2O2-次甲基绿催化分光光度法测定痕量铁(Ⅲ)
Catalytic Spectrophotometric Determination of Trace Iron ( ⅲ )
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从甲基绿与焦宁对DNA的双重结合作用所得结果表明:两种染料能以不同程度同时与DNA结合,但在此两者之间存在着一定程度的竞争作用,以甲基绿占明显的优势。
On summarizing all the results obtained a mechanism of binding of methyl green by DNA has been proposed . A competition has been observed between methyl green and pyronin in the double binding process by DNA , with the former predominating over the latter .
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方法将Feulgen法、甲基绿染色法、全染料(stainsall)法和银染法等几种非荧光DNA特异染色方法试用于彗星试验,并与溴化乙锭(EB)染色进行了比较。
Methods Some special un_fluorescent DNA staining methods , Feulgen , Methyl Green , Stains_all and Silver_Stain were tested in Comet Assay and the comparison studies with EB stain were carried out .
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方法采用甲基绿-派诺宁染色法及SABC免疫组化方法检测30例OLP及20例正常口腔黏膜(对照组)中Ki-67蛋白表达及细胞凋亡情况。
Methods Immunohistochemical staining with SABC method and methyl green-pyronine staining were used to detect the expressions of Ki-67 in 30 cases of OLP and 20 cases of normal oral mucosa specimens ( the controls ) .
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甲基绿与水溶性多壁碳纳米管的相互作用
Interaction between multi - walled carbon nanotube hydrosol and methyl green
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甲基绿-派洛宁-马休黄组合染色法检测神经元凋亡
The Method of MG-P-MY Staining in Detecting the Neuronal Apoptosis
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甲基绿-萃取光度法测定锑
Extraction - Photometric Determination of Antimony with Methyl Green
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甲基绿-钒(Ⅴ)-溴酸钾催化体系测定超痕量钒的研究
Determination of trace vanadium by catalytic spectrophotometry with methyl Green-V (ⅴ) - KBrO_3
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甲基绿选择性电极催化电位法测定茶叶中锰
Determination of Manganese in Tea by Catalytic Potentiometry with Methyl Green Ion Selective Electrode
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酸处理后甲基绿派洛宁染色法增殖型和分化型细胞的区别染色
Methyl green - pyronin staining after acid denature
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甲基绿派诺宁染色:缺血半影区细胞数量减少,结构完整,可见到少数胞浆呈紫红色且胞核呈绿色的凋亡细胞。
There were a few apoptotic neurons in ischemic penumbra under Methyl Green Pyronin staining .
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UV/Fenton试剂降解染料甲基绿的研究
Investigation of methyl green by UV-Fenton reagent
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方法:应用甲基绿-派洛宁-马休黄组合染色法,检测沙土鼠脑缺血后海马神经元的凋亡。
Methods : The neuronal apoptosis in CA1 region of hippocampus following ischemia reperfusion was detected using the MG P MY staining method which combined methyl green , pyronine and martius yellow .
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以热酸活化过的海泡石为吸附剂,研究其对亚甲基蓝、结晶紫和甲基绿3种染料的吸附动力学过程,并分析了浓度、温度等因素对海泡石吸附效果的影响。
The adsorption kinetic processes of methylene blue , crystal violet and methyl green from aqueous solution on activated sepiolite were studied , and the effects of concentration and temperature on adsorption were analyzed .
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通过整蚕(蛹)石蜡切片甲基绿-派罗宁和孚尔根染色的方法对变态期家蚕中肠、丝腺的形态结构进行显微观察。
Using the methods of paraffine-mounted sectioning and staining by methyl green-pyronine and feulgen , the morphology and structure of midgut and silk-gland of silkworm , Bombyx mori , were observed by microscopy during metamorphosis .
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研究了在酸性介质中,抗坏血酸对溴酸钾、次甲基绿、钒催化体系具有显著的活化作用,建立了测定抗坏血酸的新方法。
It is found that vitamin C has a remarkable catalytic effect on the reaction system of KBrO_3 , methylene green and vanadium - A new method for the determination of vitamin C is established based on this phenomenon .
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受精前,卵核和极核内均无孚尔根正反应颗粒,但都有甲基绿正反应的颗粒;它们的核仁均呈极强的派洛宁反应。
Before fertilization , the nuclei of egg and central cell do not show Feulgen-positive particles , but show methyl green positive particles , and the nucleoli of egg nucleus and two polar nuclei show intensely positive pyronin reaction .
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在不同温度下,聚亚甲基绿的循环伏安图上均有一阳极峰和一阴极峰,阳极峰和阴极峰的峰电流均随温度升高而增加,即电极反应速率随温度升高而增加。
There are an anodic peak and a cathodic peak in the cyclic voltammogram of poly ( methylene green ) at various temperatures . Their peak currents increase with increasing temperature , which means that the reaction rate of the electrode increases with increasing temperature .
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溴代十六烷基三甲基铵敏化亮绿脱氧核糖核酸作用的共振光散射增强研究
The Sensitizing Effect of Cetyltrimethylammonium Bromide on the Resonance Light-Scattering Enhancement Between the Interaction of Deoxyribonucleic Acids and Brilliant Green