空斑

引种试验中E.戊型肝炎病毒的空斑和空斑减少中和试验

Plaque and plaque reduction neutralization test for detection of hepatitis E virus

通过PCR检测病毒DNA，空斑实验检测病毒滴度。

Adenoviral virus DNA was detected with polymerase chain reaction ( PCR ), and plaque assay was performed to determine the titer of virus .

辅酶Q（10）的毒性试验及对溶血空斑形成细胞的刺激效应

Toxicity study on coq_ ( 10 ) and its stimulant effect on the formation of plaque-forming cells

一种用于布氏菌病研究的空斑形成细胞（PFC）测定法&Yamada试验

The method for PFC assay in brucellosis research

增加空斑形成细胞（PFC）的数量等功效。

Increase the quantity of PFC .

流行性出血热（EHF）病毒半微量甲基纤维素空斑法的建立

Development of EHF Virus Semi - micro Methylcellulose Plaque Assay

普通轮状病毒Wa的空斑实验

A plaque assay for the ordinary rotavirus wa

MTT法测定对肝癌细胞生长抑制作用，并通过空斑法测定病毒增殖，探讨其杀伤机理。

The cell growth inhibition and virus propagation efficiency was measured by MTT assay and plaque formation assay , respectively .

目的建立SARS病毒空斑测定方法，为SARS病毒生物学研究提供手段。

Objective To develop plaque assay for SARS virus , in order to provide a reliable means for SARS research .

从15株HSV-2临床株中未提取到耐药空斑；

No drug-resistant plaque was isolated from the HSV-2 clinical strains .

但草血竭水煮醇沉干浸膏与草血竭纯化部分能够明显杀灭病毒，即随药物浓度的增加，CPE特征逐渐减弱，病毒滴度也逐渐降低，而空斑减数率明显升高。

As the dose of drugs increased , viral titers decreased and plaque inhibition rate increased correspondingly .

用系列单克隆抗体、空斑减少中和试验、RTPCR及部分核苷酸序列分析等检定，确证所分离毒株的血清（或基因）型别。

The serotype of the virus was then examined by serial McAbs , PRNT , RT PCR and sequencing analysis of part M segment .

MTT分析法、空斑减数法及CPE观察法评估药物体外抗病毒效果的比较与分析

Comparison and Analysis of CPE Assay , MTT Assay and Plaque Reductive Assay for Evaluating Medicines in Antiviral Effects in vitro

免疫前和免疫后第28d分别于家兔心脏采血，分离血清运用空斑减少中和实验测定血清的中和抗体滴度。

Rabbit heart blood was colleted before immunization and 28 days post immunization .

方法以6×106空斑形成单位（PFU）RSV鼻腔滴注及腹腔注射，LMWH400IU/kg腹腔注射接种SD大鼠。

Methods SD rats were inoculated intranasally and intraperitoneally with 6 × 106 plaque forming unit ( PFU ) RSV and LMWH 400 IU / kg .

结果：虚热时交感神经兴奋，肾上腺皮质素释放增多，ANAE%，PFC空斑数增多，NK活性降低，血内纤维蛋白原增多。

Results : Deficiency of Hot syndrome group : The sympathetic N. S. excited , adrenal cortex hormone release increased , ANAE % , PFC increased , NK cell activity decreased .

目的：比较MTT分析法、空斑减数试验及细胞病变效应（CPE）观察3种方法在评估药物体外抗病毒效果分析中的优劣，为选择适宜的药物体外抗病毒效果的检测方法提供实验依据。

Objective : To compare the effect of plaque reductive assay , MTT assay and cytopathic effect ( CPE ) on evaluating the results of antiviral proliferation of medicines in vitro .

检测免疫器官重量、脾脏溶血空斑形成细胞数、E花环形成率、T淋巴细胞转化试验及白细胞介素2（IL2）活性。

The weight of immune organ , the hemolytic plaque of spleen , the rate of E rosette , the T lymphocyte transformation and the interleukin 2 activity were determined .

通过迟发型细胞免疫SRBC-DTH试验和体外溶血空斑（PFC）形成功能试验，观察辐射对小鼠免疫功能的影响；

With delayed cellular immunity SRBC-DTH and PFC tests , we investigated the effects of radiations on the immunity of mice .

结果：鲨肝活性肽S-8300可显著促进免疫抑制小鼠溶血素、溶血空斑形成及血清IL-2水平增高。

RESULT : S-8300 markedly improved the formulation of serum haemolysin , PFC and the content of IL-2 in serum of the immunosuppression mice .

结果灵芝多糖锗对小鼠溶血素形成和溶血空斑形成有显著的升高作用，对ConA、LPS诱导体外小鼠T、B淋巴细胞增殖有明显的兴奋作用。

Results Saccharum germanium of glossy ganoderma had effects on the increase of mices hemolysin and hemolysis aero - macule formation , and has stimulating effects on mices T. B lymphocytes multiplication induced by ConA , LPS .

结果表明：腹腔注射环磷酰胺80mg/kg，可以使正常小鼠的胸腺指数、血清溶血素水平、空斑形成细胞溶血能力和外周血中WBC总数下降。

The thymus index , hemolysin level , hemolytic ability of spleen plague forming cells and the number of WBC in the peripheral blood decreased by CY 80 mg / kg ip .

kg-1腹腔注射，每日1次，连续14d，检测溶血空斑数、血清溶血素水平、ConA诱导的T淋巴细胞转化率和迟发型变态反应。

The number of haemolytic empty spots , the level of serum hemolysin , the transform rate of T lymphocytes induced by ConA , delayed hypersensitivity were measured .

对肾综合征出血热（HFRS）病毒的微量细胞培养法和空斑法进行了比较。

Comparison of the micro cell culture test and the plaque assay was carried out by measuring titer of 4 strains of HFRS virus and detecting 3 neutralizing McAbs against HFRS virus .

结果显示，鱼呼肠孤病毒感染CIK细胞后，光镜下可见空斑形成；

The results showed that CIK cells infected with FRV appeared cytopathic effect ( CPE ) and plaques under optical microscope and the fragement of these cell nuclei were found .

应用空斑形成试验，花结形成试验及conA诱导脾细胞增殖试验等测定了人参茎叶总皂甙对SHR大鼠的免疫调节作用。

The effects of Ginsenoside ( GS ) on immunological regulation of the spontaneous hypertensive rats ( SHR ) were measured by plaque-forming test rossett forming test and spleen cell proliferation test induced by Con A.

为了得到分离株的阅读框（ORF），又设计了四对引物。在细胞上对最先分离到的5株病毒进行空斑纯化，进行一步法生长曲线测定。

Another 4 pairs primers were designed to clone the ORF of CPV . The strains were purified by plaque assay , and then one-step growth curves were obtained .

以细胞病变抑制法和空斑减少试验法，在不同试验策略（治疗、保护）下，评价银翘散体外抗流感病毒H3N2作用，比较2种方法不同适用条件。

Evaluated potency inhibition by the cytopathic effect ( CPE ) and the plaque reduction test method under different experimental strategies ( treatment , protection ) 4 .

APS能够显著提高正常小鼠SRBC免疫后脾细胞直接溶血空斑（d-PFC）和间接溶血空斑（i-PFC）数量，但对Tx-ATS小鼠不能促进d-PFC数升高。

APS potentiated significantly both the direct and indirect plaque-forming cells ( PFC ) in normal mice following SRBC immunization , but it did not potentiate direct PFC in T_x-ATS mice .

用空斑形成法滴定各脏器中的病毒滴度，观察药物在动物体内对HSV感染的抑制作用。结果大黄乙醇提取物经皮下注射未发现小鼠有毒性反应；

The virus titers in viscera were assayed by using plaque formation technique and the rhubarb inhibitions to the infection of HSV in vivo were observed Results No toxic response to mice were observed for REE injected hypodermically ;