等电点

目的研究缓症链球菌外毒素蛋白的分子量、等电点、氨基酸组成及N末端序列。

Purpose To determine the molecular weight , isoelectric point ( pI ), amino acid compositions and N terminal amino acid sequence of streptococcus mitis pyrogenic exotoxin ( SMPE ) .

这个ph称为等电点，蛋白质在这一点上溶解度最生物一等奖授给了班上年龄最小的孩子。

This PH is termed isoelectric point , at which proteins show their minimum solubility . The first prize for Biology went to the youngest child in the class .

在pH值大于等电点pI时频率响应较大；

A larger frequency response occurred at a pH greater than pI .

其等电点pI为4.2；

The isoelectric point was estimated to be 4.2 by IEF-PAGE .

3）等电点（pI）一致；

3 ) no change of isoelectric point ( pI ) .

并测定了葡萄籽蛋白质的等电点（PI）为3.8。

The PI of the protein is 3 . 8 .

BSA吸附量当体溶液pH值等于BSA等电点时达到最大。

It reached maximum at the isoelectric point of BSA .

采用聚丙烯酰胺凝胶盘状电泳测定其等电点（pI）为5.2。

The isoelectric point of GBV-PA was pH 5.2 by disc polyacrylamide gel eletrophoresis .

该技术是通过加入两性电解质，形成较平滑pH梯度，使样品在等电点处停止电泳。

This technology can form smooth pH gradient by adding ampholyte and make protein stop electrophoresis at the isoelectric points .

明胶浓度越高、pH值越接近等电点、胶凝温度和熔化温度越高，胶凝时间越短，同时形成的凝胶网络的弹性和凝胶强度大；

High concentration , pI and low temperature result to higher gelling point , shorter gelling time and higher gel strength .

研究了部分产物的等电点、吸湿性、保湿性和在不同pH值溶液中的溶解性；

Some products ' isoelectric point , hygroscopicity , retention characteristic and the solubility in different pH solutions are studied here .

结果经纯化后所得样品具有等电点均一性，达到了高效液相纯及SDSpage凝胶电泳纯。

China . Results HPLC , SDS PAGE and IEF electrophoresis all proved the sample 's homogeneity .

等电点pHpzc是当吸附剂表面的正电荷与其表面的负电荷数量相等时，溶液的pH值。

It is a pH value when the quantity of positive charges on a sorbent surface just equals that of negative charges .

在低于等电点下，随着pH值的下降L-精氨酸的吸附率增大；

When the pH value is below the isoelectric point , the ratio of adsorption is increasing as the value of pH decreases ;

等电点时Cu（Ⅱ）与HSA或BSA结合的研究

Studies on the binding between cu (ⅱ) and HSA or BSA at isoionic point

结果表明配基密度、pH值、盐浓度和蛋白质特性（等电点和疏水性）对内毒素去除效果均有重要影响。

The results indicated that the ligand density , pH value , salt concentration and protein property all have remarkable effects on endotoxin removal .

经SDS－PAGE测得分子量约40.3kD，等电点为5.1，N－末端氨基酸为精氨酸，酶的最适温度为35℃。

With arginine as N terminal , pI of 5 1 and molecular weight of 40 3 kd measured by SDS PAGE .

水热法制备纳米TiO2及其等电点的研究

Preparation of Nanometer Titanium Dioxide Powders by Hydrothermal Method and their Isoelectric Points

调节pH值改善体系的带电状况，能够使电势达到较大的值，避开等电点可以增加颗粒间的电位斥阻；

The electrification and potential of the solution were optimized by regulating pH values to escape iso - electric point and to increase electric repulsion .

结果相对分子质量约为43×103，等电点为8.5。N端序列不能测到。

Results The enzyme had a relative molecular mas of 43 × 103 and the pI was about 8.5.The N-terminal amino acid sequence could not be determined .

CⅡ溶液的最大吸收峰为230nm，等电点pH值约6.25。

The maximum absorption peak was at 230 nm and the pI was approximately 6.25 .

编码蛋白的理论等电点为8.52；抗原表位可能位于cDNA序列795～846处。

The academic pI was 8.52 , and the antigen determinant was probably from 795 to 846 on the cDNA sequence .

树木对SO2的抗性机理&叶细胞的pH值、等电点、缓冲容量与抗SO2的关系

Trees ' Resistance to the SO_2 in Atmosphere - a Study on Relationship between pH , Isoelectric Point , Buffer Capacity of leaf Sap and SO_2-resistance of Trees

对纯化步骤作了产率和纯度分析后，又就重组蛋白的分子量、N端序列、等电点、肽质量指纹图谱及二硫键位置等理化性质做了进一步鉴定。

After that , we identified the molecular weight , the N-terminal sequencing , isoelectric point , disulfide bonds , and other physical and chemical properties of the recombinant protein .

10.电分析法（EA）：包括示波极谱法、等电点法；

10.Electronic analysis ( EA ) including oscillopolarographic discrimination ( OD ) and isoelectric point method ( IP ) .

SDSPAGE分析表明，经等电点纯化的微纤维蛋白质的主要成分是59.5kD和63.5kD蛋白质。

As revealed by SDS_PAGE , the 59.5 kD and 63.5 kD proteins were the major components of the microfibrillar protein purified by isoelectric point precipitation .

等电点测定及PCR扩增对产ESBLs菌初步分型，然后对PCR扩增阳性产物测序，序列分析进一步确定基因型，并做结合实验。

ESBLs were confirmed with inhibitor-potentiated test . ESBLs genotypes were analyzed by isoelectric point determination , PCR , nucleotide sequencing and conjugation experiments .

优化各种混凝条件，计算了等电点处PAC与胶体间的吸附能和吸附常数。

Optimizing the coagulation condition , adsorption energy and constant between dye and coagulant were calculated isoelectric point .

结果表明，pH值极大地影响着黄粉虫幼虫蛋白质的溶出率，pH值5.8时溶出率最低，此时可能为黄粉虫蛋白的等电点；

The results showed that pH value greatly influenced the solubility , a minimum solubility was found at pH 5 . 8 . The solubility increased as the pH value increases .

一般的说，蛋白在金表面最大的吸附在分子的等电点（pI）处或再高0.5个PH值处。

In general , a protein maximally adsorbs on the gold surface at the isoelectric point ( pI ) of the molecule or0.5 pH unit higher ( 18,19 ) .