纸片法

随后进行传代培养，观察每一代细菌生长及出现耐药的情况，直至某一组产生细菌耐药现象，纸片法测定三组细菌耐药情况的差异，并测量每组的MIC值。

Subsequent subculture , observed each generation culture of bacteria , until a group of bacteria becoming drug resistance . Kirby-Bauer method was used to measure the antibiotic sensitivity and resistance of bacteria .

方法从未接受抗菌药物治疗或48h接受有效抗菌药物治疗的呼吸道感染病人中分离致病菌，采用纸片法测定细菌敏感试验结果及采用琼脂平板二倍稀释法测定最低抑菌浓度（MIC）。

Methods Bacteria isolated from respiratory tract infection patients who had not received antibacterial drug treatment or 48 hours utility treat - ment . In-vitro activity of bacteria isolates to 4 antibioties by kirby-Bauer method and minimal inhibitory con - centrations ( MIC ) were measured by agar dilution method .

结论Etest可以便捷直观地鉴定对万古霉素异质性耐药葡萄球菌的药敏结果，与琼脂平板对倍稀释法接近，优于纸片法。

Conclusion E-test can conveniently identify the heterogeneously vancomycin resistant Staphylococci similar to the agar dilution method and superior to the paper disks .

方法采用肉汤稀释法、E试验和KB纸片法进行抗生素药物敏感试验；

Method Susceptibility to antibiotics was determined by broth microdilution , E - test , and K-B disk .

用KB纸片法进行了常用抗生素的敏感性测试。

The K B paper method was used for common antibiotics sensitivity test .

头孢西丁纸片法检测mecA基因介导的MRS

Detection of Meticillin-resistant Staphylococci Mediated by mecA Gene Using Cefoxitin Disk Diffusion Method

EDTA纸片法检测大肠埃希菌和克雷伯菌的高产AmpC酶

Detection of AmpC β - lactamases in Escherichia coli and Klebsiella spp using EDTA disk

[方法]用纸片法、PCR扩增法检测产bla情况；

Methods : the state of β - Lactamase-producing was measured by paper strip method and PCR amplified method .

用头孢西丁纸片法筛查MRSA阳性82株，阴性63株。

In cefoxitin disk diffusion tests , 82 isolates showed MRSA-positive and 63 isolates were negative .

方法用Etest试条法检测铜绿假单胞菌对亚胺培南的MIC值，用KB纸片法测定对其他11种抗菌药物的敏感性。

Methods The MIC values of Pseudomonas aeruginosa to IMP were determined by E-test strips and their susceptibility to other 11 antimicrobial agents were tested by the Kirby-Bauer method .

采用WHO推荐的改良K-B纸片法，对所有菌株均进行了12种抗菌药物的药敏试验。

K-B test was employed to determine the antibiotic susceptibility of all the strains .

方法：采用K-B纸片法和梯度琼脂平板法测定细菌敏感性；

METHODS : Susceptivity to antibiotics of the bacteria was measured by K-B and agar diffusion methods .

方法：对412株革兰氏阴性杆菌采用K-B纸片法筛选试验和双纸片法表型确证试验检测ESBLs。

Methods Detect ESBLs strain during 412 gram-negative bacteria by means of K-B paper screen and double paper validating methods .

抗生素敏感性测定采用KB纸片法，最低抑菌浓度（MIC）采用琼脂二倍稀释法。

The sensibility of the bacteria to antibiotics were examined by KB paper method and the minimal inhibitory concentration by gel double multiple dilute method .

NCCLS已组织了一个工作组，目前正在进行嗜麦芽窄食单胞菌和洋葱伯克霍德菌的纸片法与MIC的评价研究。

NCCLS has organized a working group which is currently performing studies to evaluate the disk and MIC methods for S.maltophilia and B.cepacia .

结果多重PCR法对纸片法阳性株及阴性株ESBLs检出率分别为943%（33/35）和263%（5/19）；

Results 94.3 % ( 33 / 35 ) ESBLs positive strains and 26.3 % ( 5 / 19 ) ESBLs negative strains for NCCLS disk susceptibility test were positive for Multiplex PCR .

方法将临床分离的6株黄杆菌属细菌分别以纸片法、双纸片协同法检测其产BLA和ESBLs；

Methods The production of BLA and ESBLs from 6 clinical isolated flavobacterium strains was determined by nitrocefin disc test and double-disc synergy method , respectively .

采用成组匹配的病例对照研究方案，用纸片法采集病例和对照外周血标本，用PCR－RFLP方法确定基因型（C677T）。

Venous blood from both cases and controls were collected by dry blood spots . Genotype ( C677T ) of each sample was decided by PCR - RFLP method .

方法采用酸试剂纸片法检测产Blac的葡萄球菌；

METHODS Blac in Staphylococcus were detected by acid agent-paper ;

纸片法活性检测表明S227-4具有抗耐药菌活性。采用MIC测定法对该样品抗耐药活性进行研究。

The anti-drug resistant activity of S227-4 was tested in vitro by MICs assay using different drug resistant Staphylococcus aureus strains isolated clinically .

方法：连续检测2002年至2004年本院铜绿假单胞菌的耐药状况。药敏试验采用K-B纸片法，判定标准依据美国国家临床实验室标准化委员会（NCCLS）标准。

Methods K-B test was used for the antibiotics susceptibility test and the results were read based on National committee for Clinical Laboratory standards ( NCCLs ) .

结果表明，2种方法的测定结果基本一致，无显著性差异，但纸片法测定周期比多管法缩短了2d，仅需1d即可。

It shows results of this method is consistent with that of the other and havn 't significant deviation , but the period of the paper method shorten two days compared with multitube method . It only need one day .

结果用Etest法和纸片法检测362株真菌对氟康唑的总敏感性分别为779%和765%；

RESULTS The total susceptibility rate of fungi to fluconazole was 77 9 % and 76 5 % by Etest and disk diffusion method .

ATB专家系统还从75株葡萄球菌中检测出59株耐甲氧西林葡萄球菌（MRS），比原药敏结果增加了4株，但经纸片法证实仍漏检了4株苯唑西林低耐药株。

Among 75 strains of Staphylococci , 59 strains were identified as methicillin-resistant Staphylococci ( MRS ) by ATB expert system , 4 strains more than original results , but failed to report 4 strains as MRS.

方法采用K-B法药敏试验对158株葡萄球菌进行耐药性分析、琼脂扩散法测MRS、Nitrocefin纸片法测定其β-内酰胺酶。

Methods The drug-resistance of 158 Staphylococcus isolated from patients was investigated by using agar dilution test and Kirby-bauer respectively , and the β - lactates were detected by using nitrocefin method .

方法：对70例铜绿假单胞菌下呼吸道感染的临床资料进行统计分析，采用纸片法（Kirbybauer）测定该菌的体外药物敏感性。

Method : To analyze the clinical data of 70 cases with low respiratory tract infection caused by pseudomonas aeruginosa and determine drug sensitivities against PA by means of Kirby Bauer .

根据文献及临床经验确定每味药物的剂量范围，制备中药药液，进行药效学实验：杀菌实验、MIC（最小抑菌浓度）实验、纸片法（药敏实验）。

According to literature and clinical experience sure every flavour drug dose , preparation of liquids , TCM muskone experiment : antiseptic experiment , MIC ( minimum bacteriostatic concentration ) experiments and paper method ( antimicrobial susceptibility test ) .

方法用头孢西丁药敏纸片法（K-B法）和头孢西丁三维试验作AmpC酶的筛选、确证试验，对AmpC酶阳性者进行质粒接合试验。

Methods AmpC enzyme was screened by cefoxitin susceptibility disk test ( K-B method ) and confirmed by cefoxitin three-dimensional test . The strains with AmpC enzyme positive were performed by plasmid conjugation test .

头孢西丁纸片法筛选出40株MRSA，其中39株mecA基因阳性，方法特异性为97.5%，灵敏度92.9%；

Forty isolates of MRSA were detected by cefoxitin agar diffusion , in which 39 strains had mecA gene . The specificity and sensitivity of the method was 97.5 % and 92.9 % .

方法用SS琼脂及伊红美兰琼脂培养基培养阳性的沙门氏菌，采用血清凝集法鉴定分型，同时采用K-B纸片法检测药物敏感性。

Methods The positive strains cultured by Salmonella-Shigella agar ( SS ), and Eosin Methylene Blue Agar medias were identified by serum agglutination method , and the antimicrobial resistance of Salmonella enteritidis was detected by NCCLS recommended K-B disc diffusion method .