细胞纯化

CD34~+细胞纯化移植治疗高危或复发的急性淋巴细胞性白血病

CD34 + cell purification transplantation for the treatment of high-risk or recurrent acute lymphocytic leukemia

人外周血NK细胞纯化、扩增及克隆化的初步探索

Preliminary Study in Purification , Proliferation and Cloning of Human NK Cells

人外周血NK细胞纯化方法的初步比较

Preliminary study on the purification methods of human NK cells

本试验研究了不同浓度及组合消化酶对奶牛乳腺组织分离的影响以及不同FBS浓度培养基对奶牛乳腺上皮细胞纯化和生长的影响。

The purpose of the second part is to test the effect of different FBS concentrations media on bovine mammary epithelial cells culture .

结论冻干Vero细胞纯化疫苗抗体阳转率高、副反应发生率低，临床应用优于地鼠肾纯化疫苗，适宜推广使用。

Conclusion The positive conversion rate of antibody in experimental group after immunized with purified vero cell rabies vaccine for human use is high and superior to the purified shrewmouse kidney cell rabies vaccine , and suitable for popularization .

结论：建立了大鼠睾丸支持细胞纯化培养方法，支持细胞中INHα、βA、βB亚基表达均为强阳性表达，证实支持细胞能够合成、分泌INH。

Conclusion : In vitro culture method of rat Sertoli cells was successfully established . Sertoli cells in vitro were positive for inhibin α,β A and β B subunits and rat testicular Sertoli cells were capable of producing inhibin and proven to be the major source of inhibin .

细胞纯化采用差速贴壁法。

The cells were purified with an technique of differential anchoring velocity .

分离肝细胞纯化培养方法的比较研究

Comparison of the methods for isolation and purification of hepatocytes

用选择性酶消化法和机械刮除法进行细胞纯化；

The cell line was purified by selective enzyme digestion and mechanical elimination .

大鼠沃勒变性面神经施万细胞纯化培养实验研究

Purification of cultured Schwann cells derived from Wallerian degenerating facial nerves in rats

星形胶质细胞纯化培养较易；少突胶质细胞纯化培养较难。

The purification and cultivation of astrocytes are easier than that of oligodendrocytes .

体外培养雪旺氏细胞纯化方法的探讨

The Comparison of the Purity Methods of the Cultured Schwann Cell in Vitro

0.25%胰蛋白酶用于肾小管上皮细胞纯化和传代培养。

0.25 % trypsase was used in purification of renal tubular epithelial cells and sub-culture .

差速贴壁技术对大鼠脑皮质星形胶质细胞纯化率的影响

Influence of differential velocity adherent technique on the purified rate of cerebral astrocytes in rats

COBE-2991细胞纯化机纯化胰岛细胞后；

Islet cells were purified with COBE-299 cell purification machine and then cultured in vitro for 48 hours .

细胞纯化：采用人工刮除法及差速贴壁法纯化细胞。

Purification of cells : The cells were purified by using a combination of manual scraping and differential attachment techniques .

目的改良现有的小胶质细胞纯化分离培养方法，建立稳定简便的培养模型。

Objective To modify the current cultural method of rat microglia and establish a convenient and stable model of culture method for rat microglia .

目前，种植体与软组织附着的确切机制尚无定论，口腔粘膜基层上皮细胞纯化培养及种植体组织界面超薄切片技术的困难是影响这项研究的两个难题。

Difficulties in culturing purified oral mucosal basal cells and in preparing ultra - thin sections of the tissue / implant interface are two obstacles to the research in this field .

传至3代，细胞纯化，形态较为单一，呈长梭形贴壁生长，以平行排列生长为主或呈漩涡状生长。2分低糖、中糖、高糖培养基培养后，细胞趋向形成细胞团簇。

The third generation cells were purified and most of them were spindle-shaped . They showed vortex shaped or parallel arrangement growth . 2 After cultivated by low-glucose 、 mid-glucose 、 high - glucose , cell clusters were formed .

方法在取材、培养条件和细胞纯化等方面对腺瘤细胞原代培养的方法作了进一步的优化和改进。

Methods The methods of the in vitro primary cell culture of colorectal adenomas , mainly the method for taking and preparing specimen , the methods of culture conditions and cells purification , were considered again and improved in this study .

SD大鼠神经干细胞分离纯化及特性实验研究

Experimental Study on Isolation and Properties of SD Rat Neural Stem Cells

采用ELISA法检测小鼠脾细胞或纯化B细胞在抗氧化剂作用下产生IgG的情况。

IgG production in spleen cell or in purified B cells was also detected by ELISA .

外周血干细胞的纯化方法可获得高纯度和高回收率的CD34+细胞，并极大限度的去除T、B等细胞。

CD34 + cells with high purity and recovery can be isolated with concomitant effective T and B cell deletion with this purification method .

重组CHO细胞HBsAg纯化工艺的优化

Optimizing the Recombinant CHO HBsAg Purification

神经细胞裂解纯化得到GABA受体蛋白，利用SDS-聚丙烯酰胺凝胶电泳测定其分子量。

GABA receptor proteins were gained after isolated neuron cells and purification and the molecular weight of which was determined by SDS-PAGE .

方法：①将SD乳鼠在无菌条件下断头取出大脑皮质部分，进行星形胶质细胞的纯化培养，采用胶质纤维酸性蛋白免疫组化方法鉴定星形胶质细胞。

METHODS : ① The cerebral cortex of the neonatal SD rats was removed under sterile condition , the astrocytes were isolated and cultured , and then identified with the glial fibriliary acidic protein immunohistochemical staining .

这些技术主要基于对从细菌和动物细胞里纯化出的酶的使用，使得DNA复制、转化为RNA、转译为蛋白质都可以在实验室完成。

These techniques are mostly based on the use of enzymes purified from bacteria and animal cells , that allow steps such as DNA copying ( replication ), conversion into RNA ( transcription ), and into protein ( translation ), to be recapitulated in the laboratory .

目的：在NB1培养基中建立体外胚胎大鼠背根神经节（DRG）细胞的纯化培养体系。

Objective : To establish purified culture system of dorsal root ganglion ( DRG ) cells derived from embryonic rats in NBl media .

为进一步确定哺乳动物蛋白酶体的组份和相关调节蛋白质，邱小波教授等人改良了基于亲和纯化的方法来快速从哺乳动物细胞中纯化26S蛋白酶体。

To better define its composition and associated regulatory proteins , Qiu et al developed affinity methods to rapidly purify 26S proteasomes from mammalian cells .

方法用MACS（magneticcellsorting）从BALB/c小鼠静息T细胞分离纯化CD4~+CD25~+T细胞，体外细胞增殖实验观察其对CD4~+CD25~-T细胞的免疫抑制作用；

Methods CD4 ~ + CD25 ~ + regulatory T cells were isolated by MACS ( magnetic cell sorting ) from naive BALB / c mice . Their suppression on the proliferation of CD4 ~ + CD25 ~ - T cells was analyzed by cell proliferation assay .